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Lannea coromandelica is one of plant dispersed in Sulawesi. Inhabitants from the Southeast Sulawesi apply Lannea coromandelica Bark to relief symptom of gastritis or peptic ulcer. Phenolic compounds of Lannea coromandelica Stem bark contains antioxidant activity. The study investigated the gastroprotective effect of Lannea coromandelica stem bark. Ethanol extract of Lannea coromandelica Stem bark (LCSB) was evaluated its anti-ulcer activity using aspirin-induced rats. Normal group was given 0.05% Na CMC. Positive group was given sucralfat 360 mg / kgBW and aspirin 600mg/kg BW, group I was given extract of LCSB at dose 400 mg / kgBW and aspirin 600 mg/kgBW, group II was given extract of LCSB at dose 800 mg / kg BW and aspirin 600 mg/kgBW, and group III was given extract of LCSB at dose 1200 mg / kg BW and aspirin 600 mg/kg BW. Extract of LCSB was administered orally in various doses for three days prior to the ulcer induction. On the 4^th-day rats, all rats were observed 8 hours after aspirin induction to determine ulcer index and percent inhibition. Results of this study revealed that LCSB extract significantly reduced the ulcer index and percent inhibition (p=0.00). Extract of LCSB shows gastroprotective activities.

Keywords

Bark of Javanesse wood, Lannea coromandelica, Aspirin, Gastroprotective, Rat.

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Authors

Mus Ifaya ¹, Ahmad Muhtadi ², Ida Musfiroh ³, I Sahidin ⁴, Amar Ma’ruf ⁵, Syawal Abdurrahman ⁶

Affiliations
1 Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran., IN
2 Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, IN
3 Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran., IN
4 Faculty Of Pharmacy, Universitas Halu Oleo, Kendari, Southeast Sulawesi, ID
5 Departemen of Pharmacy, Faculty of Science and Technology, Mandala Waluya Kendari High School of Health Science, Southeast Sulawesi, ID
6 Departement of Medical Laboratory Technology, Mandala Waluya Kendari High School of Health Science, Southeast Sulawesi, ID

Source

Research Journal of Pharmacy and Technology, Vol 15, No 4 (2022), Pagination: 1837-1842

Abstract

Introduction: Lawsonia inermis L is a medicinal herb from Lythraceae family. This plant is proven to be able to treat various diseases. Lawsonia inermis L is one of the flowering plant of a single species of the genus Lawsonia. This plant contains the main active compounds such as flavonoids, phenols, alkaloids, glycosides, saponins, tannins, and essential oils. Flavonoids and phenols are the active compounds which most commonly found. Objective : This study aims to determine the quality parameters of the extract which include specific and non-specific parameters, antioxidant activity and total flavonoid content in purified extracts of Lawsonia inermis L. Methodology: The extract was obtained by maceration method using 96% ethanol. Purified extract was obtained by separating the chlorophyll and tannins contained in the sample using ethanol and aqudes, then continued by partitioning using ethyl acetate and adding MgSO₄ as a drying agent. The ethyl acetate phase was concentrated using a Rotary Vacuum Evaporator to obtain a purified extract with a reddish black color. Furthermore, the extract was analyzed using thin layer chromatography (TLC) using eluent n-hexane: ethyl acetate (1: 1). Result : Specific parameters include organoleptic observations, water-soluble compounds and ethanol-soluble compounds, while non-specific parameters include drying loss, moisture content, total ash content, acid soluble content, specific gravity, microbial contamination, mold/yeast contamination, Pb metal contamination, Cd and As. The antioxidant activity assay used the DPPH method and the IC50 value was 39.07 mg / L, while the total flavonoid test used quercetin as a comparison and the total flavonoid content was 3.29%. Conclusion : The purified extract of Lawsonia inermis L. leaves comply the quality standard of the extract, has very strong antioxidant activity and contains flavonoids.

Keywords

Lawsonia inermis L, Standardization, Radical DPPH, Total Flavonoids.

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