Research Journal of Pharmacy and Technology

Mohammad Shamim Qureshi ¹, A. Venkateshwar Reddy ², G. S. Kumar ³

Affiliations
1 Jawaharlal Nehru Technological University, Kukatpally Hyderabad, - 50035, Telangana, IN
2 Anwarul Uloom College of Pharmacy, New Mallepally, Hyderabad – 500001, Telangana, IN
3 School of Pharmacy, International Medical University, Bukit Jallil, Kuala Lumpur 57000, MY

Abstract

The present study was designed to investigate the Antiulcer activity of methanolic extract of the leaves of Hydrolea zeylanica (L) Vahl using two different models namely Ethanol and cold restraint stress induced ulcers models in rats. The extract was administered at a dose of 250 and 500 mg/kg orally 30 min prior to ulcer induction. Ranitidine (50 mg/kg) was used as a reference standard. The antiulcer activity was accessed by determining and comparing the ulcer index in the test group with that of the standard group. Gastric volume, total acid and free acid were estimated in rats. Hydrolea zeylanica leaf methanolic extract (HZLME) (500 mg/kg) showed maximum inhibition of gastric acid, free acid and total acid respectively. The ulcer index in the Hydrolea zeylanica leaf methanolic extract treated animals was found to be significantly less in all the models compared to standard drug treated group. However the antiulcer activity of Hydrolea zeylanica leaf methanolic extract was, found to be lesser than that of ranitidine. The results suggest that Hydrolea zeylanica possesses significant antiulcer property which could be due to cytoprotective action of the drug or strengthening of gastric and duodenal mucosa with an enhancement of mucosal defence mechanism.

Keywords

Hydrolea zeylanica, Cold Restraint Stress, Total Acidity, Ulcer Index.

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Santosh Kumar Panda ¹, A. Venkateshwar Reddy ², Mohammad Shamim Qureshi ²

Affiliations
1 Singhania University, Pacheri Bari, Jhunjhunu, Rajasthan-333515., IN
2 Anwarul Uloom College of Pharmacy, New Mallepally, Hyderabad-500001., IN

Abstract

Ziziphus jujubahas been used for many years for its medicinal and culinary properties. The present study aimed to investigate the antiepileptogenic activity of methanolic extract of Ziziphus jujubaleaves (MEZJ) and subsequently its neuro protective action against excitotoxicity and reactive oxygen species (ROS) generation in the brain of pentylenetetrazole (PTZ) –induced kindled mice. MEZJ was studied for its suppressive action on convulsion and seizure scores (antiepileptogenic effect) and protection of neurons against PTZ-induced oxidative stress injury (neuro protective effect) when given during kindling acquisition as a pretreatment before every PTZ injection. A major antiepileptic drug Diazepam was also tested for comparison. Both MEZJ and Diazepam showed antiepileptogenic activity as they caused reduction in development of seizure scores and reduced the sensitivity of kindled mice to the convulsive and lethal effects of PTZ. MEZJ and Diazepam significantly decreased oxidative stress injury in the mice brain as compared to PTZ-kindled group. MEZJ showed more neuroprotective action as compared to Diazepam as it enhanced the antioxidant enzyme levels more in the mice brain. The results obtained from the study support the hypothesis that neuro protective action of MEZJ not only correlate with its ability to inhibit ROS formation but also for its ability to suppress seizure generation.

Keywords

Antiepileptogenic, Neuroprotective, Oxidative Stress, MEZJ, Diazepam, PTZ, Kindled, Seizure Scores.

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