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Hadwan, Mahmoud Hussein
- The effect of Morbid Obesity on the Liver Function Enzymes
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Authors
Affiliations
1 Environmental Research and Studies Centers, Babylon University -51002, IQ
2 Babylon Technical Institute, Al-Furat Al-Awsat Technical University, 51015, Hilla, IQ
3 Chemistry Dept., College of Science, University of Babylon, Iraq, Hilla, IQ
1 Environmental Research and Studies Centers, Babylon University -51002, IQ
2 Babylon Technical Institute, Al-Furat Al-Awsat Technical University, 51015, Hilla, IQ
3 Chemistry Dept., College of Science, University of Babylon, Iraq, Hilla, IQ
Source
Research Journal of Pharmacy and Technology, Vol 12, No 5 (2019), Pagination: 2241-2244Abstract
Background: The liver is the major organ in the body and it is considered as an important organ for protein manufacture and detoxification. Liver health is evaluated by study liver function (LFTs) which include TSB, AST, ALT and ALK. The current study is an attempt to understand the correlation between morbid obesity and liver function enzymes. Method: The research population consisted of 67 subjects that divided into two groups; morbidly obese group (41) and the control group (healthy appearance subjects) (26). The levels of serum liver enzymes activities (ALT, AST, ALK) and TSB were determined by suitable kits. Result: In current study no statistically significant differences were observed in AST, ALT and ALK levels between the two types of procedures. The highest differences were noted for TSB levels between obese and individual healthy. While there was an association between the BMI and both AST and ALT; there was no relationship between the body mass index BMI and TSB and ALK. Conclusion: This study showed a correlation between the BMI with AST and ALT of morbidly obese.Keywords
Obesity, GPT, GOT, ALK, TSB.References
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- Precise Spectrophotometric Method for Measurement of Peroxiredoxin Activity in Biological Samples
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Authors
Affiliations
1 Chemistry Dept., Kufa University, Najaf City, Najaf Governorate, IQ
2 Chemistry Dept., College of Science, University of Babylon, Hilla City, Babylon Governorate, P.O. 51002, IQ
1 Chemistry Dept., Kufa University, Najaf City, Najaf Governorate, IQ
2 Chemistry Dept., College of Science, University of Babylon, Hilla City, Babylon Governorate, P.O. 51002, IQ
Source
Research Journal of Pharmacy and Technology, Vol 12, No 5 (2019), Pagination: 2254-2260Abstract
Herein, we describe a simple spectrophotometric method for the measurement of peroxiredoxin activity and demonstrate reproducibility, accuracy, and precision. In these experiments, peroxiredoxin activity was measured by incubating enzyme samples with phosphate buffer solution containing suitable concentrations of the substrates 1,4-dithio-DL-threitol (DTT) and hydrogen peroxide. Titanium sulfate was added to stop enzyme reactions, and subsequent reactions with residual hydrogen peroxide produced pertitanic acid, which was spectrophotometrically measured at 405 nm. Advantages of this method are including the elimination of catalase interference and allowing application of this method to all types of biological tissues. The peroxiredoxin assay is simple and can be completed with few additions. The method is precise, with coefficients of variation of 2.93% within runs and 5.4% between runs. Data from the present peroxiredoxin assay were strongly correlated with those from the ferrous oxidation–xylenol orange (FOX) method (r = 0.9835).Keywords
Peroxiredoxin, Pertitanic Acid, FOX Reagent, Dithiothreitol, H2O2.References
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