Research Journal of Pharmacy and Technology

Bulusu Ravi Teja ¹, Bhupathi Raju Kishan Varma ¹, Chandaka Prasanna Kumar ¹, Mukthinuthalapati Mathrusri Annapurna ¹

Affiliations
1 Department of Pharmaceutical Analysis & Quality Assurance, GITAM Institute of Pharmacy,GITAM University, Visakhapatnam-530045, IN

Abstract

Three simple spectrophotometric techniques have been developed for the quantification of Fluorometholone in ophthalmic preparations using sodium acetate buffer (pH 4), phosphate buffer (pH 4) and phosphate buffer (pH 7) solutions. Linearity was observed over the concentration range 2-25, 2-30 and 1-50 μg/mL in sodium acetate buffer, phosphate buffer (pH 4) and phosphate buffer (pH 7) respectively. The three methods were validated and can be applied for the assay of Fluorometholone in pharmaceutical formulations.

Keywords

First Order Derivative Spectrophotometry, Fluorometholone, Validation.

Full Text