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Tedjo, Aryo
- In Silico Study and In Vitro test of Extract Kaempferia pandurata Roxb. as Anti ER (+) Breast Cancer Cell Line MCF-7
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Authors
Fadilah
1,
Lowilius Wiyono
2,
Brenda Cristie Edina
2,
Risya Amelia Rahmawati
2,
Linda Erlina
1,
Aryo Tedjo
1,
Rafika Indah Paramita
3
Affiliations
1 Departement of Medical Chemistry, University of Indonesia, ID
2 University of Indonesia, ID
3 Indonesian Medical Education and Research Institute, University of Indonesia, ID
1 Departement of Medical Chemistry, University of Indonesia, ID
2 University of Indonesia, ID
3 Indonesian Medical Education and Research Institute, University of Indonesia, ID
Source
Research Journal of Pharmacy and Technology, Vol 12, No 5 (2019), Pagination: 2391-2395Abstract
Breast cancer most commonly occurs in women worldwide, in 2012 there were 1.670.000 cases with the second most prevalence in the world (25% of the total incidence of cancer). Drugs used so far have not been effective and many resistance occurs, it is necessary to a discovery of herbs that contain polyphenols as anticancer. Kaempferia pandurata Roxb plant has been identified to contain various essential oils and several flavonoid compounds that have demonstrated many biological activities. This research was done by simulation study with molecular docking approach which then extraction, the result of the extract was tested in vitro activity against Breast Cancer Cell Line MCF-7. The docking results show that the flavonoid compound against the target ERbreast cancer has an enthalpy energy ΔG -11.75 kcal/mol, the phytochemical results support that the flavonoids compound dominates the composition of the Kaempferia pandurata Roxb. The Result of in vivo test with MTT method IC50 value of standard cisplatin, hexane extract was 8.42; 26.70 μg/ml respectively to MCF-7 cell line showed that Kaempferia pandurata Roxb extract very potential as an antibreast cancer.Keywords
Molecular Docking, Phytochemical Test, Antibreast Cancer, Kaempferia pandurata Roxb., MCF-7 Cell Line.References
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- The Inhibition of Ethanol Extract of Phaleria macrocarpa Stem Bark on COX-2 Expression of HCT116 Colorectal Cancer Cell Line
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Authors
Ratu Shafira Hanifah
1,
Nadia Aida Novitarani
1,
Fadhilah Harmen
1,
Aryo Tedjo
2,
Norma Nur Azizah
2,
Rista Putrianingsih
2,
Wilzar Fachri
3,
Kusmardi Kusmardi
4
Affiliations
1 Universitas Indonesia, Salemba, Jakarta, ID
2 Department of Medical Chemistry, Universitas Indonesia, Salemba, Jakarta, ID
3 Researcher of Cluster Drug Development, Indonesian Medical Education and Research Institute, Universitas Indonesia, Salemba, Jakarta, ID
4 Department of Pathology Anatomy, Universitas Indonesia, Salemba, Jakarta, ID
1 Universitas Indonesia, Salemba, Jakarta, ID
2 Department of Medical Chemistry, Universitas Indonesia, Salemba, Jakarta, ID
3 Researcher of Cluster Drug Development, Indonesian Medical Education and Research Institute, Universitas Indonesia, Salemba, Jakarta, ID
4 Department of Pathology Anatomy, Universitas Indonesia, Salemba, Jakarta, ID
Source
Research Journal of Pharmacy and Technology, Vol 12, No 6 (2019), Pagination: 2902-2906Abstract
Objective of this research to determine the inhibition of ethanol extract of Phaleria macrocarpa Boerl. stem bark on COX-2 expression in colorectal cancer cell line HCT116. Ethanol extract of Phaleria macrocarpa stem bark was prepared for maceration in ethanol. Phaleria macrocarpa stem bark in-vitro anticancer activity was tested with MTT assay on HCT116 colorectal cancer cell line. This study also assessed the effect of ethanol extract of Phaleria macrocarpa stem bark on the inhibition of COX-2 expression in the HCT116 colorectal cancer cell line by counting the H-score from immunocytochemistry staining with help from ImageJ and TMARKER application. Ethanol extract of Phaleria macrocarpa stem bark shows anticancer activity by inhibiting its growth with IC50 of 1,327 μg/ml. One of the pathways is through inhibition of COX-2 expression, shown by the H-score of 173,33 after administration of Phaleria macrocarpa stem bark at a dose of 200 ppm. This study shows that Phaleria macrocarpa stem bark is a colorectal cancer growth inhibitor, one of which is through inhibition of COX-2.Keywords
COX-2, Colorectal Cancer, IC50, Immunocytochemistry, MTT Assay, Phaleria macrocarpa.References
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