Research Journal of Pharmacy and Technology

Suman Saha ¹, Amit Roy ¹, Sanjib Bahadur ¹, Ananta Choudhury ²

Affiliations
1 Department of Pharmaceutics, Columbia Institute of Pharmacy, Raipur, Chhattisgarh, IN
2 Department of Pharmaceutics, Assam Down Town University, Assam, IN

Abstract

This study intended to explore the influence of formulation factors on the physico-chemical properties of quercetin-loaded liposomes and optimize the fabrication environment. Thin film hydration technique was employed to prepare liposome and optimization was done by 32 factorial designs combined with desirability function. Nine preparations were prepared by using altered drug: lipid and soyphosphatidylcholine: cholesterol (SPC: cholesterol) ratios and assessed for entrapment efficacy and vesicle size. The findings were the mean diameter and drug encapsulation efficiency. Results exhibited that SPC concentration and SPC: cholesterol molar ratio had a solid impact on liposome size. Increasing the lipid ratio produced a reduction in size. The degree of quercetin charging depended on the factors evaluated. Increasing SPC concentration and lipid ratio pointed lyboosted quercetin entrapment. However, higher quercetin concentrations had a negative effect on drug entrapment. Based on this, an optimized design was determined, prepared and investigated. The entrapment efficiency and vesicle size were found to be very adjacent with the predicted values. The formulation was found to be globular shape and also shows sustained release pattern. These results backing the fact that 32 full factorial designs with desirability function might be efficiently used in optimization of quercetin loaded liposome. The overall results showed that SPC concentration and lipid ratio were the key features influencing particle size, while entrapment efficiency was affected primarily by quercetin concentration.

Keywords

Fabrication, In-Vitro Evaluation, Liposomal Quercetin.

Full Text

     

Himal Barakoti ¹, Ananta Choudhury ¹, Biplab Kumar Dey ¹

Affiliations
1 Pharmaceutical Sciences, Assam down Town University, Guwahati–781026, IN

Abstract

Self-micro emulsifying drug delivery system (SMEDDS) has been one of the exclusive approaches to improve solubility and increase bioavailability for the poorly aqueous soluble drug. Due to microstructural features of active entity and formulation as a whole, vigorous researches are being carried to explore the significance and possibility of this drug delivery system at present. SMEDDS is an isotropic mixture of oil, surfactant, co-surfactant and water which involves encapsulation of drug into the lipid base and the formulation as a whole have unique ability to form fine o/w emulsion upon gentle agitation. The whole emulsification process requires only little entropy change which is obtained from the peristaltic motion of gut. Due to microstructural features of active entity and formulation as a whole provided by this approach, vigorous researches are being done at present time to interrelate the in vitro results obtained with in vivo responses. Considering the fact that 40% of the newly discovered drugs are lipophilic in nature, it signifies that the study in this approach will continue and more commercial formulation will be made available in near future. This review gives an overview on SMEDDS with sequential demystification thus enabling a greater understanding of their present role in medicine and drug delivery.

Keywords

Self-Emulsifying, Drug Delivery System, Novel Approach, Lipophilic Drug.

Full Text

     

Ananta Choudhury ¹, Suman Saha ², Sanjib Bahadur ², Amit Roy ²

Affiliations
1 Assam Down Town University, Panikhaiti, Gandhinagar, Guwahati-26 Assam, IN
2 Columbia Institute of Pharmacy, Tekari, Raipur C.G., IN

Abstract

The research work was mainly designed to determine the antifungal activity of curcumin, a potent bioactive phytoconstituent, obtained from Curcuma longa and to explore the possibilities of its use as a combination with commercially available synthetic antifungal drugs for better therapeutic actions. Fluconazole and itraconazole were used as a model drug. Different combinations of curcumin with fluconazole as well as itraconazole was prepared and subjected to an antifungal screening study. The antifungal screening was carried out using Candida albicans fungal strain. The MIC of fluconazole, itraconazole and curcumin was found to be in the range of 32 μg/ml to 64 μg/ml, 8 μg/ml to 32μg/ml and 64 μg/ml to 256 μg/ml respectively. Further, the results of the invitro antifungal study performed based on the comparative zone of inhibition measurement of the prepared combination at a concentration of 10 μg/ml were determined. The result of the study indicates that the presence of curcumin significantly increases the antifungal capacity of both fluconazole and itraconazole. The Fractional inhibitory concentration index was measured, and the data thus obtained, states that the increased antifungal activity may be observed due to synergistic or additive effects. Further, the topical sensitivity of the optimized combinations was determined using rabbit vaginal model and were found to be free from any major sign of sensitivity as per as histopathological study concern.

Keywords

FICI, Natural Antifungal, Candida albican, Curcumin, Antifungal Combination.

Full Text