Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

A New Pharmacological Model for Hepatocellular Carcinoma in Channa Punctatus and its Pharmaco Networking Studies


Affiliations
1 Amity Institute of Pharmacy, Amity University, NOIDA, India
2 Amity Institute of Biotechnology, Amity University, NOIDA, India
3 Department of Pharmaceutical Chemistry, Jamia Hamdard, Hamdard University, New Delhi, India
     

   Subscribe/Renew Journal


Thioacetamide (TAA) is a well-known reported carcinogen for the induction of liver cancer. Different animal models have been developed to investigate different stages of HCC in rats, mice and guinea pigs. Use of these animals for new chemical entity leads to large number of deaths, sufferings and pain. Hence to reduce the number of animals while screening new moieties, use of fishes for establishing a diseased model is a novel and better concept. In the present study we report a Novel protocol for the induction of hepatocellular carcinoma in Channa Punctatus. After induction of TAA in the span of 21 days, different stages of HCC were observed. Moreover it was also found that AST, ALT and ALP levels were in elevated state. Further pharmaconetworking studies were established using STRING and it was found that important biological processes are disturbed in the presence of Thioacetamide.

Keywords

Thiacetamide, Hepatocellular Carcinoma, Channa Punctatus, STRING.
Subscription Login to verify subscription
User
Notifications
Font Size


  • AV Bondre, SC Akare, P Mourya, AD Wanjari, PS Tarte, GV Paunikar. In Vitro Cytotoxic Activity of Leaves of Abutilon indicum Linn. Against Ehrlich Ascites Carcinoma and Dalton’s Ascitic Lymphoma Cell Line. Research J. Pharmacognosy and Phytochemistry 2009; 1(1): 72-74.

  • Christian Liedtke et al; Experimental liver fibrosis research: update on animal models, legal issues and translational aspects; Fibrogenesis and Tissue Repair2013; 6-19.

  • Deepak Rao Khadatkar, Yogesh Rathore . An Efficient and Useful Hybrid Approach for Detection of Lung Cancer. Research J. Engineering and Tech. 2(4):Oct.-Dec. 2011 page199-202.

  • Dibyajyoti Saha, Tarashankar Maity, Mayukh Jana, Supradip Mandal. Cancer Treatment Strategy-An Overview. Asian J. Pharm. Tech. 1(2): April-June 2011; Page 28-33.

  • Edokwe Chinelo, Obidoa Onyechi, Joshua Parker Elijah. Biochemical Effects of Persea americana (Avocado) and Dacryodes edulis (African Pear) Extracts on Liver Function Test of Albino Wistar Rats. Research J. Science and Tech. 2010; 2(2): 34-37 .

  • Froese R et al 2010: FishBase. World Wide Web elec-tronic publication. Available at: http://www.fishbase.org (accessed on 10 October 2010).

  • Harit G et al ; Endosulfan Uptake and biochemical profile of the muscle in a freshwater murrel Channa punctatus; Bulletin of Pure and Applied Sceinces , 2007, 26A; 23-27.

  • Jae-Sang Hong et al ; MicroRNA signatures associated with thioacetamide-induced liver fibrosis in mice ; Bioscience, Biotechnology, and Biochemistry, 2017 ; Vol. 81, No. 7, 1348–1355.

  • Kumbhar Swapnil, Salunkhe Vijay , Magdum Chandrakant. Targeted Drug Delivery: A Backbone for Cancer Therapy. Asian J. Pharm. Res. 3(1): Jan.-Mar. 2013; Page 40-46.

  • M. Afzal Khan et al ; Morphometric variation of snakehead fish, Channa punctatus, populations from three Indian rivers; Journal of Applied Ichthyology 29(3) · June 2013.

  • Mallikarjuna RaoTalluri et al ; Thioacetamide induced acute liver toxicity in rats treated with Balanites Roxburghii extracts; Journal of Acute Disease, Vol5(5), 2016; 413-418.

  • MC Wallace et al ; Standard operating Procedures in Experimemtal Liver Research ; Thioacetamide model in mice and rats, Laboratory Animals; 2015, Vol. 49(S1) 21–29.

  • Nachiket S Dighe, Shashikant R Pattan, Deepak S Musmade, Abhijeet N Merekar, Manisha S Kedar, Deepak K Thakur, Pratik V Patel. Recent Synthesis of Marine Natural Products with Anticancer Activity: An Overview. Research J. Science and Tech. 2009; 1(2): 63-70 .

  • Natarajan K., Ramanathan S., Sathish R. , C. Amudhadevi. In Vitro Cytotoxic Study on Crude Extract of Marine Invertebrate Animal Polyclinum madrasensis Sebastian. Asian J. Pharm. Tech. 1(4): Oct. - Dec. 2011; Page 152-154.

  • Nilantika Paul et al ; Lead toxicity on non-specific immune mechanisms of freshwater fish Channa Punctatus; Aquatic Toxicology ; Volume 152, July 2014, Pages 105-112.

  • Raghav M. , Dixit S. Diversity of Fishes in a Freshwater Reservoir of Chhattisgarh, India. Research J. Science and Tech. 2009; 1(2): 103-107.

  • Ramachandran P; Liver fibrosis: a bidirectional model of fibrogenesis and resolution. QJM 2012, 105:813–817.

  • S. K. Sahu, D. Das, N. K. Tripathy. Hepatoprotective activity of aerial part of Glinus oppositifolius L. against Paracetamol-induced Hepatic Injury in Rats. Asian J. Pharm. Tech. 2(4): Oct. - Dec. 2012; Page 154-156.

  • S. H. Baie et al ; The wound healing properties of Channa striatus-cetrimide cream -wound contraction and glycosaminoglycan measurement,” Journal of Ethno pharmacology, vol.73,no.1-2,pp.15–30,2000.

  • Sanjay Pandey et al ; Acute toxicity bioassays of mercuric chlorideand malathion on air breathing fish Channa punctatus(Bloch); Ecotoxicology and Environmental safety; Volume 61, Issue 1, May 2005, Pages 114-120.

  • Schaeck M et al; Fish as research tools: alternatives to in vivo experiments; Alternative Lab Animals ; 2013 Jul;41(3):219-29.

  • Sonali K Doke et al; Alternatives to animal testing: A review; Saudi Pharmaceutical Journal; 2015 Jul; 23(3): 223–229.

  • Steven J. Cooke et al; On the sustainability of inland fisheries: Finding a future for the forgotten; Ambio. 2016 Nov; 45(7): 753–764.

  • Tasleem Akhtar et al ; An overview of thioacetamide - induced hepatotoxicity; Journal Toxin Reviews , Volume 32, 2013, Issue3, 43-46.

  • Thirumurugan D, Vijayakumar R.. Exploitation of Antibacterial Compound Producing Marine Actinobacteria against Fish Pathogens Isolated from Less Explored Environments. Asian J. Pharm. Res. 3(2): April- June 2013; Page 75-78.


Abstract Views: 282

PDF Views: 0




  • A New Pharmacological Model for Hepatocellular Carcinoma in Channa Punctatus and its Pharmaco Networking Studies

Abstract Views: 282  |  PDF Views: 0

Authors

Shikha Saxena
Amity Institute of Pharmacy, Amity University, NOIDA, India
Sohini Singh
Amity Institute of Biotechnology, Amity University, NOIDA, India
Sandhya Bawa
Department of Pharmaceutical Chemistry, Jamia Hamdard, Hamdard University, New Delhi, India
Deepshikha Pande Katare
Amity Institute of Biotechnology, Amity University, NOIDA, India

Abstract


Thioacetamide (TAA) is a well-known reported carcinogen for the induction of liver cancer. Different animal models have been developed to investigate different stages of HCC in rats, mice and guinea pigs. Use of these animals for new chemical entity leads to large number of deaths, sufferings and pain. Hence to reduce the number of animals while screening new moieties, use of fishes for establishing a diseased model is a novel and better concept. In the present study we report a Novel protocol for the induction of hepatocellular carcinoma in Channa Punctatus. After induction of TAA in the span of 21 days, different stages of HCC were observed. Moreover it was also found that AST, ALT and ALP levels were in elevated state. Further pharmaconetworking studies were established using STRING and it was found that important biological processes are disturbed in the presence of Thioacetamide.

Keywords


Thiacetamide, Hepatocellular Carcinoma, Channa Punctatus, STRING.

References