Research Journal of Pharmacy and Technology

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L-Asparaginase from Soil actinomycetes:Optimization of Process Variables using Plackett Burman Method


Affiliations
1 Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal-576104, Udupi, Karnataka, India
2 Department of Pharmaceutical Chemistry, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal-576104, Udupi, Karnataka, India
     

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Extensive exploration of soil microorganisms in search of novel metabolites have edified researchers on the available natural products for drug discovery. Actinomycetes are filamentous Gram positive bacteria capable of producing wide array of bioactive metabolites. High GC content in the DNA, makes the organism unique and peculiar from other microorganisms. The present article contains data on purification and characterization of L-asparaginase from soil actinomycetes. Soil samples were collected from different locations in Udupi fish market, Karnataka, India. A total of 106 isolates were screened through rapid plate assay method, out of which 17 were found active. Furthermore, a promising isolate FMCL-13A with maximum enzyme activity was chosen by submerged fermentation technique. Statistical designs viz., Placket-Burman (PB) was employed for optimization of various experimental parameters in order to enhance L-asparaginase production. The nutritional variables which affects the enzyme production by submerged fermentation from the active isolate was optimized by PB model.

Keywords

Actinomycetes, L-Asparaginase, Placket Burman Model, Optimization.
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  • B. Meena, L. Anburajan, T. Sathish, R. Vijaya Raghavan, G. Dharani, N. Valsalan Vinithkumar, R. Kirubagaran, L-Asparaginase from Streptomyces griseus NIOT-VKMA29: Optimization of process variables using factorial designs and molecular characterization of l-asparaginase gene, Sci. Rep. 5 (2015) 12404. doi:10.1038/srep12404.

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  • R. Usha, K.K. Mala, C.K. Venil, M. Palaniswamy, Screening of Actinomycetes from Mangrove ecosystem for L-asparaginase Activity and optimization by response surface methodology, Polish J. Microbiol. 60 (2011) 213–221.

  • A.M. Farag, S.W. Hassan, E.A. Beltagy, M.A. El-Shenawy, Optimization of production of anti-tumor l-asparaginase by free and immobilized marine Aspergillus terreus, Egypt. J. Aquat. Res. 41 (2015) 295–302. doi:10.1016/j.ejar.2015.10.002.


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  • L-Asparaginase from Soil actinomycetes:Optimization of Process Variables using Plackett Burman Method

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Authors

Mithilesh Kumar
Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal-576104, Udupi, Karnataka, India
Anuraag Muralidharan
Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal-576104, Udupi, Karnataka, India
Aravinda Pai
Department of Pharmaceutical Chemistry, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal-576104, Udupi, Karnataka, India
V. M. Subrahmanyam
Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal-576104, Udupi, Karnataka, India
Venkatesh Kamath
Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal-576104, Udupi, Karnataka, India

Abstract


Extensive exploration of soil microorganisms in search of novel metabolites have edified researchers on the available natural products for drug discovery. Actinomycetes are filamentous Gram positive bacteria capable of producing wide array of bioactive metabolites. High GC content in the DNA, makes the organism unique and peculiar from other microorganisms. The present article contains data on purification and characterization of L-asparaginase from soil actinomycetes. Soil samples were collected from different locations in Udupi fish market, Karnataka, India. A total of 106 isolates were screened through rapid plate assay method, out of which 17 were found active. Furthermore, a promising isolate FMCL-13A with maximum enzyme activity was chosen by submerged fermentation technique. Statistical designs viz., Placket-Burman (PB) was employed for optimization of various experimental parameters in order to enhance L-asparaginase production. The nutritional variables which affects the enzyme production by submerged fermentation from the active isolate was optimized by PB model.

Keywords


Actinomycetes, L-Asparaginase, Placket Burman Model, Optimization.

References