Research Journal of Pharmacy and Technology

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Golden apple snail Eggs Extract:Biosynthesis of Nanoparticles and Its Antibacterial effect


Affiliations
1 Department of Biology, College of Science, Baghdad University, Baghdad, Iraq
     

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Finding alternatives for antibiotics has been a very interesting topic nowadays because of bacterial resistance to a wide range of antibiotics, thus studies began to investigate other bactericidal material to solve such problem, In this study silver nanoparticles (AgNPs) have been synthesized using a biological source from the golden snail egg extract. The AgNPs was detected by scanning probe microscopy and showed average grain size of 58.98 nm in diameter, it also showed antibacterial activity against Gram positive (Staphylococcus aureus, Staphylococcus epidemidis, Streptococcus pyogens) and Gram negative (Salmonella, Escherichia coli, Klebsiella, Pseudomonas aeruginosa and Enterobacter) bacteria by well diffusion assay and tube dilution method. Antibiofilm activity was detected by crystal violate staining assay through the use of AgNPs synthesized in this study and results showed very good inhibition in biofilm production at low concentrations of AgNPs.

Keywords

Apple Snail Egg Extract, Silver Nanoparticles, Green Synthesis, Antibacterial Activity.
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  • Golden apple snail Eggs Extract:Biosynthesis of Nanoparticles and Its Antibacterial effect

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Authors

M. S. Ghada
Department of Biology, College of Science, Baghdad University, Baghdad, Iraq
S. N. Shaymaa
Department of Biology, College of Science, Baghdad University, Baghdad, Iraq
M. Abass
Department of Biology, College of Science, Baghdad University, Baghdad, Iraq

Abstract


Finding alternatives for antibiotics has been a very interesting topic nowadays because of bacterial resistance to a wide range of antibiotics, thus studies began to investigate other bactericidal material to solve such problem, In this study silver nanoparticles (AgNPs) have been synthesized using a biological source from the golden snail egg extract. The AgNPs was detected by scanning probe microscopy and showed average grain size of 58.98 nm in diameter, it also showed antibacterial activity against Gram positive (Staphylococcus aureus, Staphylococcus epidemidis, Streptococcus pyogens) and Gram negative (Salmonella, Escherichia coli, Klebsiella, Pseudomonas aeruginosa and Enterobacter) bacteria by well diffusion assay and tube dilution method. Antibiofilm activity was detected by crystal violate staining assay through the use of AgNPs synthesized in this study and results showed very good inhibition in biofilm production at low concentrations of AgNPs.

Keywords


Apple Snail Egg Extract, Silver Nanoparticles, Green Synthesis, Antibacterial Activity.

References