Research Journal of Pharmacy and Technology

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Antibacterial Activity of Essential Oils of Rosmarinus officinalis, Salvia officinalis and Anthemis nobilis Widespread in the Syrian Coast


Affiliations
1 Department of Microbiology and Biochemistry, Al Andalus University, Tartous, Syrian Arab Republic
2 Department of Pharmacognosy and Phytochemistry, Al Andalus University, Tartous, Syrian Arab Republic
3 Department of Pharmaceutical Chemistry and Quality Control, Al Andalus University, Tartous, Syrian Arab Republic
     

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We aimed in this study to determine the antibacterial efficacy of essential oils of Rosmarinus officinalis, Salvia officinalis leaves and Anthemis nobilis flowers widespread in the Syrian coast against several strains of Staphylococcus aureus and Pseudomonas aeruginosa. The essential oils were extracted and the yield was measured using Clevenger apparatus. In vitro investigation of antibacterial activity using agar disc diffusion method and minimal inhibitory concentration (MIC) of each essential oil was determined by microdilution method against studied bacteria. Results demonstrated that Rosmarinus officinalis essential oil had the highest activity (MIC for Staphylococcus aureus was 3.9μl/ml and MIC for Pseudomonas aeruginosa was 7.8 μl/ml). Salvia officinalis essential oil had moderate antibacterial effect with MIC of 31.2 μl/ml and 125μl/ml against Staphylococcus aureus and Pseudomonas aeruginosa respectively. On the other hand, Anthemis nobilis essential oil had no efficacy against tested bacterial strains. According to our study, Rosmarinus officinalis essential oil exhibited higher antibacterial activity against both Staphylococcus aureus and Pseudomonas aeruginosa than this of Salvia officinalis.

Keywords

Rosemary, Sage, Chamomile, Essential Oils, Antibacterial Activity, MIC.
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  • Miladinović D., Miladinović LJ. Antimicrobial activity of essential oil of sage from Serbia. Physics, Chemistry and Technology Vol. 2, No 2, 2000: pp. 97 - 100.

  • Buchbauer, G., Jirovetz, L. Aromatherapy-Use of Fragrances and Essential Oils as Medicaments. Fla Vour Fragrance J., 9; 1994: 217–222.

  • Bozin, B.; Mimica-Dukic, N., Simin, N., Anackov, G. Characterization of the Volatile Composition of Essential Oils of Some Lamiaceae Species and the Antimicrobial and Antioxidant Activities of the Entire Oils. J. Agric. Food Chem. 54; 2006: 1822–1828.

  • Valero M, Salmerón MC. Antibacterial activity of 11 essential oils against Bacillus cereus in tyndallized carrot broth. Int J Food Microbiol. 85; 2003:73–81.

  • Bruneton, J.. Pharmacognosy, Phytochemistry, Medicinal Plants, 2nd ed.; Intercept Ltd.: London; 1999: pp 539–540.

  • Tadrent W, Bachari K, Kabouche Z. Comparative Compositions and Antibacterial Activity of the essential oils of Anthemis nobilis l. and Anthemis mixta l. (asteraceae). International Journal of Pharmacy and Pharmaceutical Sciences. Vol 8, Issue 7; 2016: 457-59.

  • Al-Snafi A E. Medical importance of Anthemis nobilis (Chamaemelum nobile) - A Review. AJPST. 6(2); 2016: 89-95.

  • Harfouch R.M., Mohammad R., Suliman H. Antibacterial activity of Syrian propolis extract against several strains of bacteria in vitro. World J. Pharm. Pharmaceuti. Sci., 6 (2016), pp. 42-46.

  • Zam W, Harfouch R, Bittar S, Sayegh M. Antibacterial activity of various Syrian honey types against Pseudomonas aueruginosa. Research Journal of Pharmacognosy and Phytochemistry 2017; 9(2):73-76.

  • Fu Y, Zu Y, Chen L, Shi X, Wang Z, Sun S, Efferth T. Antimicrobial activity of clove and rosemary essential oils alone and in combination. Phytother Res. Oct; 21(10); 2007: 989-94.

  • National Committee for Clinical Laboratory Standards (NCCLS). Performance standards for antimicrobial susceptibility testing. Twelfth International Supplement. Wayne, PA, 2002: pp.M100-S12.

  • Hussain A, Anwar F, Chatha A, Jabbar A, Mahboob S, and Nigam P. Rosmarinus officinalis essential oil: antiproliferative, antioxidant and antibacterial activities. Brazilian Journal of Microbiology, 41; 2010: 1070-1078.

  • Bozin B, Mimica-Dukic N, Samojlik I, Jovin E. Antimicrobial and Antioxidant Properties of Rosemary and Sage (Rosmarinus officinalis L. and Salvia officinalis L., Lamiaceae) Essential Oils J. Agric. Food Chem. 55; 2007:7879–7885.

  • Viljoen A, Vuuren SV, Ernst E, Klepser M, Demirci B, Baser H, Wyk BEV. Osmitopsis asteriscoides (Asteraceae)-the antimicrobial and essential oil composition of a Cape-Dutch remedy. J Ethnopharmacology 88; 2003:137–143.


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  • Antibacterial Activity of Essential Oils of Rosmarinus officinalis, Salvia officinalis and Anthemis nobilis Widespread in the Syrian Coast

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Authors

Rim M. Harfouch
Department of Microbiology and Biochemistry, Al Andalus University, Tartous, Syrian Arab Republic
Manal Darwish
Department of Pharmacognosy and Phytochemistry, Al Andalus University, Tartous, Syrian Arab Republic
Wisam Al-Asadi
Department of Pharmacognosy and Phytochemistry, Al Andalus University, Tartous, Syrian Arab Republic
Ali F. Mohammad
Department of Pharmaceutical Chemistry and Quality Control, Al Andalus University, Tartous, Syrian Arab Republic
Nour M. Gharib
Department of Microbiology and Biochemistry, Al Andalus University, Tartous, Syrian Arab Republic
Mohammad Haroun
Department of Pharmaceutical Chemistry and Quality Control, Al Andalus University, Tartous, Syrian Arab Republic

Abstract


We aimed in this study to determine the antibacterial efficacy of essential oils of Rosmarinus officinalis, Salvia officinalis leaves and Anthemis nobilis flowers widespread in the Syrian coast against several strains of Staphylococcus aureus and Pseudomonas aeruginosa. The essential oils were extracted and the yield was measured using Clevenger apparatus. In vitro investigation of antibacterial activity using agar disc diffusion method and minimal inhibitory concentration (MIC) of each essential oil was determined by microdilution method against studied bacteria. Results demonstrated that Rosmarinus officinalis essential oil had the highest activity (MIC for Staphylococcus aureus was 3.9μl/ml and MIC for Pseudomonas aeruginosa was 7.8 μl/ml). Salvia officinalis essential oil had moderate antibacterial effect with MIC of 31.2 μl/ml and 125μl/ml against Staphylococcus aureus and Pseudomonas aeruginosa respectively. On the other hand, Anthemis nobilis essential oil had no efficacy against tested bacterial strains. According to our study, Rosmarinus officinalis essential oil exhibited higher antibacterial activity against both Staphylococcus aureus and Pseudomonas aeruginosa than this of Salvia officinalis.

Keywords


Rosemary, Sage, Chamomile, Essential Oils, Antibacterial Activity, MIC.

References