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Expression of FGF-2 and Fibronectin in Citrus limon Fruit Peel Malang Essential Oil Gel Treated Traumatic Ulcer in Diabetic Wistar Rats (Rattus novergicus)


Affiliations
1 Department of Oral Medicine, Airlangga University, Jl. Mayjend Prof Dr. Moestopo 47 Surabaya, East Java, Indonesia
     

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Diabetes Mellitus is a metabolic disease that delayed traumatic ulcer healing process. Citrus limon contains fumaric acid, d-limonene and citral that can accelerate the healing of traumatic ulcer. The aims of this study were to investigate the effect of 0.78% Citrus limon essential oil topical application can increase Fibroblast Growth Factor-2 (FGF-2) and Fibronectin expression. This study was true experimental analytic study with random sampling methods. The study was conducted on 30 male Wistar rats (Rattus novergicus) induced with DM by injecting 50 mg/kg of Streptozotocin, intraperitoneally, and a traumatic ulcer on their lower lip mucosa. These were divided into six groups; three each for control and treatment groups. Each control and treatment group consisted of five rats. The control groups treated with CMC 5% gel and treatment groups were administered with Citrus limon peel essential oil gel. The expression of FGF-2 and Fibronectin was observed on Days 5, 7 and 9. Furthermore, mice sacrificed and the lower lip labial mucosa tissue of mice has been taken to make the Histopathology Anatomy preparation by means of immunohistochemical examination with monoclonal antibodies anti-FGF2 and anti-Fibronectin. ANOVA analysis was performed to analyze the difference between group (p<0.05). The increased FGF-2 and Fibronectin expression in the treatment group as compared to that of the control group. There were significant differences (p<0.05) of both FGF2 and Fibronectin expression between the two groups. The citrus limon peel essential oil gel increased the FGF-2 and Fibronectin expression during the healing process of traumatic ulcers on the oral mucosa of diabetes afflicted Wistar rats (Rattus novergicus).

Keywords

Diabetes Mellitus, Citrus limon Essential Oil Gel, Traumatic ulcer, Fibroblast Growth Factor-2, Fibronectin, Herbal Medicine.
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  • Expression of FGF-2 and Fibronectin in Citrus limon Fruit Peel Malang Essential Oil Gel Treated Traumatic Ulcer in Diabetic Wistar Rats (Rattus novergicus)

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Authors

Raziv Ganesha
Department of Oral Medicine, Airlangga University, Jl. Mayjend Prof Dr. Moestopo 47 Surabaya, East Java, Indonesia
Iwan Hernawan
Department of Oral Medicine, Airlangga University, Jl. Mayjend Prof Dr. Moestopo 47 Surabaya, East Java, Indonesia
Hening Tuti Hendarti
Department of Oral Medicine, Airlangga University, Jl. Mayjend Prof Dr. Moestopo 47 Surabaya, East Java, Indonesia
Desiana Radithia
Department of Oral Medicine, Airlangga University, Jl. Mayjend Prof Dr. Moestopo 47 Surabaya, East Java, Indonesia
Priyo Hadi
Department of Oral Medicine, Airlangga University, Jl. Mayjend Prof Dr. Moestopo 47 Surabaya, East Java, Indonesia
Nurina Febriyanti Ayuningtyas
Department of Oral Medicine, Airlangga University, Jl. Mayjend Prof Dr. Moestopo 47 Surabaya, East Java, Indonesia
Diah Savitri Ernawati
Department of Oral Medicine, Airlangga University, Jl. Mayjend Prof Dr. Moestopo 47 Surabaya, East Java, Indonesia

Abstract


Diabetes Mellitus is a metabolic disease that delayed traumatic ulcer healing process. Citrus limon contains fumaric acid, d-limonene and citral that can accelerate the healing of traumatic ulcer. The aims of this study were to investigate the effect of 0.78% Citrus limon essential oil topical application can increase Fibroblast Growth Factor-2 (FGF-2) and Fibronectin expression. This study was true experimental analytic study with random sampling methods. The study was conducted on 30 male Wistar rats (Rattus novergicus) induced with DM by injecting 50 mg/kg of Streptozotocin, intraperitoneally, and a traumatic ulcer on their lower lip mucosa. These were divided into six groups; three each for control and treatment groups. Each control and treatment group consisted of five rats. The control groups treated with CMC 5% gel and treatment groups were administered with Citrus limon peel essential oil gel. The expression of FGF-2 and Fibronectin was observed on Days 5, 7 and 9. Furthermore, mice sacrificed and the lower lip labial mucosa tissue of mice has been taken to make the Histopathology Anatomy preparation by means of immunohistochemical examination with monoclonal antibodies anti-FGF2 and anti-Fibronectin. ANOVA analysis was performed to analyze the difference between group (p<0.05). The increased FGF-2 and Fibronectin expression in the treatment group as compared to that of the control group. There were significant differences (p<0.05) of both FGF2 and Fibronectin expression between the two groups. The citrus limon peel essential oil gel increased the FGF-2 and Fibronectin expression during the healing process of traumatic ulcers on the oral mucosa of diabetes afflicted Wistar rats (Rattus novergicus).

Keywords


Diabetes Mellitus, Citrus limon Essential Oil Gel, Traumatic ulcer, Fibroblast Growth Factor-2, Fibronectin, Herbal Medicine.

References