Research Journal of Pharmacy and Technology

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Exploring the Protective Effect of Ascorbic Acid on Amoxicillin and Clavulanic Acid-Induced Lipid Peroxidation in Goat Liver Homogenate


Affiliations
1 Columbia Institute of Pharmacy, Near Vidhan Sabha, Tekari, Raipur, Chhattisgarh-493111, India
2 SLT Institute of Pharmaceutical Sciences, Guru Ghasidas Vishwavidyalaya, Koni, Bilaspur, Chhattisgarh,-495009., India
     

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Background: Therapeutic effect of ascorbic acid on amoxicillin and clavulanic acid-induced lipid peroxidation was carried out in got liver homogenates to investigate the relationship between drug-induced toxicity and drug-induced lipid peroxidation. Methods: The level of malondialdehyde, reduced glutathione and nitric oxide were estimated in control, drug-treated, drug-antioxidant treated and the only antioxidant-treated group at two hours and six hours of incubation time. Results: The level of malondialdehyde in the drug-treated group was found to be increased whereas the level of both reduced glutathione and nitric oxide decreased when compared to control. In the drug-antioxidant treated group, the level of malondialdehyde was found to be reduced whereas the extent of reduced glutathione, nitric oxide increased when compared to the drug-treated group. Conclusion: The present study established the potential of amoxicillin and clavulanic acid to induce lipid peroxidation. On the other hand, ascorbic acid repressed lipid peroxidation to a substantial level. Lipid peroxidation initiation capability of amoxicillin and clavulanic acid might be a contributing factor for their toxicity.

Keywords

Malondialdehyde, Nitric Oxide, Reduced Glutathione, Lipid Peroxidation, Amoxicillin, Clavulanic Acid, Ascorbic Acid, Antibiotics.
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  • Exploring the Protective Effect of Ascorbic Acid on Amoxicillin and Clavulanic Acid-Induced Lipid Peroxidation in Goat Liver Homogenate

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Authors

Bibhas Pandit
Columbia Institute of Pharmacy, Near Vidhan Sabha, Tekari, Raipur, Chhattisgarh-493111, India
Trilochan Satapathy
Columbia Institute of Pharmacy, Near Vidhan Sabha, Tekari, Raipur, Chhattisgarh-493111, India
Pooja Tiwari
SLT Institute of Pharmaceutical Sciences, Guru Ghasidas Vishwavidyalaya, Koni, Bilaspur, Chhattisgarh,-495009., India

Abstract


Background: Therapeutic effect of ascorbic acid on amoxicillin and clavulanic acid-induced lipid peroxidation was carried out in got liver homogenates to investigate the relationship between drug-induced toxicity and drug-induced lipid peroxidation. Methods: The level of malondialdehyde, reduced glutathione and nitric oxide were estimated in control, drug-treated, drug-antioxidant treated and the only antioxidant-treated group at two hours and six hours of incubation time. Results: The level of malondialdehyde in the drug-treated group was found to be increased whereas the level of both reduced glutathione and nitric oxide decreased when compared to control. In the drug-antioxidant treated group, the level of malondialdehyde was found to be reduced whereas the extent of reduced glutathione, nitric oxide increased when compared to the drug-treated group. Conclusion: The present study established the potential of amoxicillin and clavulanic acid to induce lipid peroxidation. On the other hand, ascorbic acid repressed lipid peroxidation to a substantial level. Lipid peroxidation initiation capability of amoxicillin and clavulanic acid might be a contributing factor for their toxicity.

Keywords


Malondialdehyde, Nitric Oxide, Reduced Glutathione, Lipid Peroxidation, Amoxicillin, Clavulanic Acid, Ascorbic Acid, Antibiotics.

References