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Polymorphism of IL-4 (-590) and Il-6 (-174) is not Associated with Chronic Periodontitis in Babylonian Population


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1 University of Babylon, Department of Biology, Iraq
     

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Aim: Study was aimed to find the relationship between chronic periodontitis with polymorphism of IL-4 and IL-6 genes and their concentration in saliva and serum.

Material and method: the study was included patients with chronic periodontitis (60 subjects) and healthy control (40 subjects), bacteria associated with both subjects have been isolated. The genetic variation in the sequence of the IL-4 and IL-6 has been detected by single strand conformation polymorphism (SSCP) technique. the level of IL-4 and IL-6 in saliva and serum was measured by enzyme linked immunosorbent assay (ELISA).

Results: The total viable count of bacteria that collect from patients with chronic periodontitis and healthy control showed that There was significant differences (increase in the number of bacteria) between study groups in the aerobic bacteria , the number of bacteria in healthy groups was 3.1×103±39.15 CFU/ml, and in patient was 2.4×105 ±65.93 CFU/ml P<0.001 and the anaerobic bacteria in healthy and patient was 2.3×102±22.61 CFU/ml and 1.2×104±45.95 CFU/ml respectively (P≤ 0.05).The most common bacteria isolated in this study were P. Porphyromonasgingivalis, P .Prevotellaintermedia, Tannerellaforsythensis, Peptostreptococcus spp., Aggregatibacter actinomycetemcomitans, Streptococcus spp. And Staphylococcus aureus. SSCP technique showed that there weren’t any sequence variation between patients with periodontitis and healthy control subjects in the sequence of IL-4 and IL-6 genes in Babylonian population.

Conclusion: Polymorphism of IL-4 and IL-6 is not associated with chronic periodontitis in Babylonian population.


Keywords

Musculoskeletal Symptoms, Ambulance Workers, Helmets, Fatigue, Stress of Neck and Back.
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  • Polymorphism of IL-4 (-590) and Il-6 (-174) is not Associated with Chronic Periodontitis in Babylonian Population

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Authors

Frial Gemeel Abd
University of Babylon, Department of Biology, Iraq

Abstract


Aim: Study was aimed to find the relationship between chronic periodontitis with polymorphism of IL-4 and IL-6 genes and their concentration in saliva and serum.

Material and method: the study was included patients with chronic periodontitis (60 subjects) and healthy control (40 subjects), bacteria associated with both subjects have been isolated. The genetic variation in the sequence of the IL-4 and IL-6 has been detected by single strand conformation polymorphism (SSCP) technique. the level of IL-4 and IL-6 in saliva and serum was measured by enzyme linked immunosorbent assay (ELISA).

Results: The total viable count of bacteria that collect from patients with chronic periodontitis and healthy control showed that There was significant differences (increase in the number of bacteria) between study groups in the aerobic bacteria , the number of bacteria in healthy groups was 3.1×103±39.15 CFU/ml, and in patient was 2.4×105 ±65.93 CFU/ml P<0.001 and the anaerobic bacteria in healthy and patient was 2.3×102±22.61 CFU/ml and 1.2×104±45.95 CFU/ml respectively (P≤ 0.05).The most common bacteria isolated in this study were P. Porphyromonasgingivalis, P .Prevotellaintermedia, Tannerellaforsythensis, Peptostreptococcus spp., Aggregatibacter actinomycetemcomitans, Streptococcus spp. And Staphylococcus aureus. SSCP technique showed that there weren’t any sequence variation between patients with periodontitis and healthy control subjects in the sequence of IL-4 and IL-6 genes in Babylonian population.

Conclusion: Polymorphism of IL-4 and IL-6 is not associated with chronic periodontitis in Babylonian population.


Keywords


Musculoskeletal Symptoms, Ambulance Workers, Helmets, Fatigue, Stress of Neck and Back.

References