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Evaluation of Ipomoea reniformis for Antimicrobial Activity.


Affiliations
1 School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Kalinga Nagar, Bhubaneswar, Odisha, India
2 GRY Institute of Pharmacy, Borawan, Khargone, India
     

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Scientific validation of much medicinal flora is lacking in India and still many species are used traditionally. From many years Ipomoea reniformis was studied for its traditionally claimed activity and still it is unexplored. The plant can be further explored for the study of traditionally claimed unexplored activities, as well as isolation and identification of active constituents may lead to new findings. In the present study whole plant of I. reniformis was collected, dried and extracted with various solvents according to solubility index, and finally five extracts were studied by well diffusion assay for their antifungal and antimicrobial effects on both gram positive, gram negative bacteria and a fungal species. The study revealed that ethyl acetate extract was having highest activity that is why fractions of the ethyl acetate extract was prepared by various solvents and again the antimicrobial study was done with each fraction. The ethyl acetate extract and fraction exhibited activity against both Gram positive and Gram negative bacteria, while the aqueous extracts elucidated antimicrobial activity against only Staphylococcus aureus. The ethanolic and chloroform extracts showed inhibition of Aspergillus niger. The plant showed antimicrobial activity, evidencing the need to conduct further studies that can identify their active components responsible for the activity.

Keywords

Ipomoea reniformis, Antimicrobial Activity, LCMS, IR and NMR.
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  • Bhattacharyajee SK. Handbook of Medicinal plants, P. No. 146– 148.
  • Al-Bari MAA, Sayeed MA, Rahman MS. Characterization and antimicrobial activities of a phenolic acid derivative produced by Streptomyces bangladeshiensis, a novel species collected in Bangladesh. Res. J. Med. and Med. Sci. 14 (1); 2006: 77-81
  • Ghazghazi H, Chedia A, Safa H, Wissem M, Antibacterial, antifungal and antioxidant activities of Tunisian Olea Eurpaea SSP. Oleaster Fruit pulp and its essential fatty acids. International Journal of Pharmacy and pharmaceutical sciences. 2015; 52-55.
  • Jay JM. Modern Food Microbiology. Maryland. 2000; 679.
  • Bakhiet AO, Adam SE. An estimation of Citrullus colocynthis toxicity for chicks. Vet. Hum. Toxicology. 37; 1995: 356-359.
  • Chowdhury AKA, Ali MS, Khan MO. Fitoterapia. 68; 1997: 379-380.
  • Islam S. Sweet potato (Ipomoea batatas L.) Leaf: Its Potential Effect on Human Health and Nutrition. J. Food Science. 71; 2006: R13-R21.
  • Adsul VB. Antimicrobial activities of Ipomoea carnea leaves. Scholars Research Library journal of natural products and plant resources. 2; 2012: 597-600.
  • Akueshi CO, Kadiri CO, Akueshi EU, Agina SE, Ngurukwem B. Antimicrobial potentials of hyptis sauvedens Poit (Lamiaccae). Nigeria. J. Bot. 15; 2002: 37-41.
  • Farnsworth, N.R. Biological and phytochemical screening of plants. Journal of Pharmaceutical Sciences. 55; 1966:225-276.
  • Bhatt Mehul K., Dholwani Kishore K., Saluja Ajay K. Mini Review Ipomoea reniformis: a scientific review. International Journal of Pharmacy and pharmaceutical sciences. 2010; 22-23.
  • Jorgensen J. H. and Turnidge. Susceptibility Test methods Dilution and Disk diffusion methods. Manual of clinical Microbiology. 2007; 1152 ‐1173.
  • S. Sasidharan, Y. Chen, D. Saravanan, K. M. Sundram, and L. Yoga Latha. Extraction, Isolation and Characterization of Bioactive Compounds from Plant Extracts. Afr J Tradit Complement Altern Med. 8 (1); 2010: 1–10.
  • Cahit A., Gulsen S. Antibacterial activity of crude methanolic extracts and its fractions of aerial parts of Anthemis Tinctoria. Indian journal of Biochemistry and Biophysics. 2005: 395-397.

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  • Evaluation of Ipomoea reniformis for Antimicrobial Activity.

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Authors

Ajay Raghuvanshi
School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Kalinga Nagar, Bhubaneswar, Odisha, India
D. M. Kar
School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Kalinga Nagar, Bhubaneswar, Odisha, India
Prabhat Das
GRY Institute of Pharmacy, Borawan, Khargone, India
Renu Bala
GRY Institute of Pharmacy, Borawan, Khargone, India

Abstract


Scientific validation of much medicinal flora is lacking in India and still many species are used traditionally. From many years Ipomoea reniformis was studied for its traditionally claimed activity and still it is unexplored. The plant can be further explored for the study of traditionally claimed unexplored activities, as well as isolation and identification of active constituents may lead to new findings. In the present study whole plant of I. reniformis was collected, dried and extracted with various solvents according to solubility index, and finally five extracts were studied by well diffusion assay for their antifungal and antimicrobial effects on both gram positive, gram negative bacteria and a fungal species. The study revealed that ethyl acetate extract was having highest activity that is why fractions of the ethyl acetate extract was prepared by various solvents and again the antimicrobial study was done with each fraction. The ethyl acetate extract and fraction exhibited activity against both Gram positive and Gram negative bacteria, while the aqueous extracts elucidated antimicrobial activity against only Staphylococcus aureus. The ethanolic and chloroform extracts showed inhibition of Aspergillus niger. The plant showed antimicrobial activity, evidencing the need to conduct further studies that can identify their active components responsible for the activity.

Keywords


Ipomoea reniformis, Antimicrobial Activity, LCMS, IR and NMR.

References