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Extraction and Isolation of Bioactive Molecule Lycopene from Water Melon and Evaluation of Anti Diabetic Activity Against STZ Induced Rats


Affiliations
1 Department of Pharmacy, Meera Bai Institute of Technology, Maharani Bagh, New Delhi-110065., India
2 Department of Pharmaceutical Chemistry, Teja College of Pharmacy, Kodad, Nalgonda-508206, Telangana State, India
3 Department of Pharmaceutical Chemistry, Sana College of Pharmacy, Kodad, Nalgonda-508206, Telangana State, India
4 Department of Pharmacology, Avanthi Institute of Pharmaceutical Sciences, Guthapally, Hyderabad-501512, India
5 Department of Pharmaceutical Analysis and Quality Assurance, Avanthi Institute of Pharmaceutical Sciences, Guthapally, Hyderabad-501512, India
     

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Despite the popular belief that watermelon is just water and sugar, watermelon is actually a nutrient dense food. It provides high levels of vitamins, minerals, and antioxidants and just a small number of calories. Water melons have become synonymous with summer and picnics, and for good reason. Their refreshing quality and sweet taste help to combat the heat and provide a guilt-free, low maintenance dessert. Along with cantaloupe and honeydew, watermelons are a member of the botanical family Cucurbitaceae. There are five common types of watermelon: seeded, seedless, mini (also known as personal), yellow, and orange. The objective of the present research work was to evaluate in vivo anti diabetic activity of ethanolic extract of water melon (EE-WMN).The present experimental data displayed the blood glucose level 283.5±0.4238–284.2±0.02217 mg/dL mg/dL in STZ induced diabetic rats, which was considered as severe diabetes in the glibenclamide (5 mg/kg) and EE-WMN (100, 200 and 300 mg/kg) treated groups, the peak values of blood sugar and cholesterol and triglycerides significantly reduced on the 21st day, respectively. Hence, this experimental data displayed that the EE-WMN significantly decrease the blood glucose level in diabetic rats but values did not return to those of normal controls. Therefore, the EE- WMN executed anti diabetic activity, when compared with diabetic control. From the present experimental data, here we concluded that EE-WMN displayed potential anti diabetic activity against streptozocin induced rats at 200 and 300 mg/kg. b. wt which was proved by assessment of bioanalytes from serum and urine sample and this anti diabetic activity was mainly due to the presence of carotenoids lycopene which was characterized by UV, FT-IR, 1H and 13C-NMR, APCI, HPLC spectrometry.

Keywords

Anti Diabetic, STZ, Triglycerides, FT-IR and APCI etc
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  • Extraction and Isolation of Bioactive Molecule Lycopene from Water Melon and Evaluation of Anti Diabetic Activity Against STZ Induced Rats

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Authors

Monica Chopra
Department of Pharmacy, Meera Bai Institute of Technology, Maharani Bagh, New Delhi-110065., India
Asish Bhaumik
Department of Pharmaceutical Chemistry, Teja College of Pharmacy, Kodad, Nalgonda-508206, Telangana State, India
A. Gopi Reddy
Department of Pharmaceutical Chemistry, Sana College of Pharmacy, Kodad, Nalgonda-508206, Telangana State, India
P. Sravan Kumar
Department of Pharmacology, Avanthi Institute of Pharmaceutical Sciences, Guthapally, Hyderabad-501512, India
B. Srikant
Department of Pharmaceutical Analysis and Quality Assurance, Avanthi Institute of Pharmaceutical Sciences, Guthapally, Hyderabad-501512, India

Abstract


Despite the popular belief that watermelon is just water and sugar, watermelon is actually a nutrient dense food. It provides high levels of vitamins, minerals, and antioxidants and just a small number of calories. Water melons have become synonymous with summer and picnics, and for good reason. Their refreshing quality and sweet taste help to combat the heat and provide a guilt-free, low maintenance dessert. Along with cantaloupe and honeydew, watermelons are a member of the botanical family Cucurbitaceae. There are five common types of watermelon: seeded, seedless, mini (also known as personal), yellow, and orange. The objective of the present research work was to evaluate in vivo anti diabetic activity of ethanolic extract of water melon (EE-WMN).The present experimental data displayed the blood glucose level 283.5±0.4238–284.2±0.02217 mg/dL mg/dL in STZ induced diabetic rats, which was considered as severe diabetes in the glibenclamide (5 mg/kg) and EE-WMN (100, 200 and 300 mg/kg) treated groups, the peak values of blood sugar and cholesterol and triglycerides significantly reduced on the 21st day, respectively. Hence, this experimental data displayed that the EE-WMN significantly decrease the blood glucose level in diabetic rats but values did not return to those of normal controls. Therefore, the EE- WMN executed anti diabetic activity, when compared with diabetic control. From the present experimental data, here we concluded that EE-WMN displayed potential anti diabetic activity against streptozocin induced rats at 200 and 300 mg/kg. b. wt which was proved by assessment of bioanalytes from serum and urine sample and this anti diabetic activity was mainly due to the presence of carotenoids lycopene which was characterized by UV, FT-IR, 1H and 13C-NMR, APCI, HPLC spectrometry.

Keywords


Anti Diabetic, STZ, Triglycerides, FT-IR and APCI etc

References