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Biocompatible Formulation of Probiotic Bacteria Bacillus subtilis with Agriculture Based Products for the Effective Acid Tolerance Properties and Synergistic Activity with Antibiotics


Affiliations
1 Department of Biotechnology, Sathyabama University, Jeppiaar Nagar, Old Mamallapuram Road, Sholinganallur, Chennai 600 119, Tamil Nadu, India
2 Department of Chemical Engineering, Sathyabama University, Chennai 600119, India
3 Centre for Bioresource and Development (C-BIRD), Department of Biotechnology, Sathyabama University, Chennai 600119, Tamil Nadu, India
     

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The present study was aimed to evaluate the acid tolerance properties and compatibility with antibacterial antibiotics of probiotic bacterial strain Bacillus subtilis formulated with various agriculture based products like potato peel and biogel. Bacterial strain was isolated from butter milk and the isolated probiotic strain was identified based on morphological, cultural and molecular characteristics which reveals the isolated strain belong to B.subtilis and the pure culture was formulated with the respective agriculture based products under aseptic condition. Acid tolerance study was carried out by determination of growth of respective formulation under different pH levels (6.5,4.5,2.5) which shows all the tested formulation retained the maximum viability in all the tested pH under all the incubation time. Compatibility of respective formulation with antibiotics was also investigated by measuring the zone of inhibition. Growth inhibition was not recorded in the majority of the antibiotics which reveals the best compatibility of formulation with the antibiotics which would suggests the possible utilization of formulated B.subtilis as an effective probiotic strain.

Keywords

Bacillus subtilis, Probiotics, Formulation, Acid Tolerance, Compatibility, Antibiotics.
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  • Biocompatible Formulation of Probiotic Bacteria Bacillus subtilis with Agriculture Based Products for the Effective Acid Tolerance Properties and Synergistic Activity with Antibiotics

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Authors

S. Karthick Raja Namasivayam
Department of Biotechnology, Sathyabama University, Jeppiaar Nagar, Old Mamallapuram Road, Sholinganallur, Chennai 600 119, Tamil Nadu, India
S. Nivash Kumar
Department of Chemical Engineering, Sathyabama University, Chennai 600119, India
S. Chandra Mohan
Centre for Bioresource and Development (C-BIRD), Department of Biotechnology, Sathyabama University, Chennai 600119, Tamil Nadu, India
V. Gayathri
Centre for Bioresource and Development (C-BIRD), Department of Biotechnology, Sathyabama University, Chennai 600119, Tamil Nadu, India

Abstract


The present study was aimed to evaluate the acid tolerance properties and compatibility with antibacterial antibiotics of probiotic bacterial strain Bacillus subtilis formulated with various agriculture based products like potato peel and biogel. Bacterial strain was isolated from butter milk and the isolated probiotic strain was identified based on morphological, cultural and molecular characteristics which reveals the isolated strain belong to B.subtilis and the pure culture was formulated with the respective agriculture based products under aseptic condition. Acid tolerance study was carried out by determination of growth of respective formulation under different pH levels (6.5,4.5,2.5) which shows all the tested formulation retained the maximum viability in all the tested pH under all the incubation time. Compatibility of respective formulation with antibiotics was also investigated by measuring the zone of inhibition. Growth inhibition was not recorded in the majority of the antibiotics which reveals the best compatibility of formulation with the antibiotics which would suggests the possible utilization of formulated B.subtilis as an effective probiotic strain.

Keywords


Bacillus subtilis, Probiotics, Formulation, Acid Tolerance, Compatibility, Antibiotics.

References