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Fabrication and In-Vitro Evaluation of Liposomal Quercetin and its Optimization


Affiliations
1 Department of Pharmaceutics, Columbia Institute of Pharmacy, Raipur, Chhattisgarh, India
2 Department of Pharmaceutics, Assam Down Town University, Assam, India
     

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This study intended to explore the influence of formulation factors on the physico-chemical properties of quercetin-loaded liposomes and optimize the fabrication environment. Thin film hydration technique was employed to prepare liposome and optimization was done by 32 factorial designs combined with desirability function. Nine preparations were prepared by using altered drug: lipid and soyphosphatidylcholine: cholesterol (SPC: cholesterol) ratios and assessed for entrapment efficacy and vesicle size. The findings were the mean diameter and drug encapsulation efficiency. Results exhibited that SPC concentration and SPC: cholesterol molar ratio had a solid impact on liposome size. Increasing the lipid ratio produced a reduction in size. The degree of quercetin charging depended on the factors evaluated. Increasing SPC concentration and lipid ratio pointed lyboosted quercetin entrapment. However, higher quercetin concentrations had a negative effect on drug entrapment. Based on this, an optimized design was determined, prepared and investigated. The entrapment efficiency and vesicle size were found to be very adjacent with the predicted values. The formulation was found to be globular shape and also shows sustained release pattern. These results backing the fact that 32 full factorial designs with desirability function might be efficiently used in optimization of quercetin loaded liposome. The overall results showed that SPC concentration and lipid ratio were the key features influencing particle size, while entrapment efficiency was affected primarily by quercetin concentration.

Keywords

Fabrication, In-Vitro Evaluation, Liposomal Quercetin.
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  • Chouhan M, Saha S, Roy A. Curcumin: A review. J Appl Pharm Res 2014; 11:18-28.
  • Lakhanpal P, Rai D. Quercetin: A versatile flavonoid. Internet J Med Update 2007;2: 22-37.
  • Mulholland PJ, Ferry DR, Anderson D, Hussain SA, Young AM, Cook JE. Pre-clinical and clinical study of QC12, a water-soluble, pro-drug of quercetin. Ann Oncol 2001; 12:245-8.
  • Saha S, Roy A, Bahadur S, Chandrakar S, Choudhury A, Das S. Phytochemicals as adjuvant in neoplasia. Asian J Res Chem 2014; 7:765-70.
  • Gao Y, Wanga Y, Mab Y, Yua A, Caia F, Shaoa W, et al. Formulation optimization and in situ absorption in rat intestinal tract of quercetin loaded micro emulsion. Coll Surf B 2009; 71:306-14.
  • Ader P, Wessmann A, Wolffram S. Bioavailability and metabolism of the flavonol quercetin in the pig. Free Radic Biol Med 2000; 28:1056-67.
  • Zhao L, Shi Y, Zou S, Sun M, Li L, Zhai G. Formulation and In Vitro evaluation of quercetin loaded polymeric micelles composed of pluronicP123 and D-a-tocopheryl polyethylene glycol succinate. J Biomed Nanotechnol 2011; 7:358-65.
  • Bhalerao SS, Harshal AR. Preparation, Optimization, Characterization, and Stability Studies of Salicylic Acid Liposomes. Drug Develop Indus Pharm 2003; 29:451-67.
  • Roy A, Saha S, Bahadur S, Choudhury A. Bioenhancement of Curcumin by Combined Approaches of Adjuvants and Liposomal Fabrication. Asian J of Pharmaceutics Oct-Dec 2016 (Suppl). 10 (4) | S688
  • Van Dijk C, Driessen AJ, Recourt K. The uncoupling efficiency and affinity of flavonoids for vesicles. Biochem Pharmacol 2000; 60:1593-600.
  • Taira MC, Chiaramoni NS, Pecuch KM, Alonso-Romanowski S. Stability of Liposomal Formulations in Physiological Conditions for Oral Drug Delivery. Drug Deliv 2004; 11:123-8.
  • Saha S, Roy A, Bahadur S, Choudhury A. Bioenhamcement of Curcumin by Dual approach. Research J. Pharm. and Tech 2016; 9(8):1059-1063.

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  • Fabrication and In-Vitro Evaluation of Liposomal Quercetin and its Optimization

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Authors

Suman Saha
Department of Pharmaceutics, Columbia Institute of Pharmacy, Raipur, Chhattisgarh, India
Amit Roy
Department of Pharmaceutics, Columbia Institute of Pharmacy, Raipur, Chhattisgarh, India
Sanjib Bahadur
Department of Pharmaceutics, Columbia Institute of Pharmacy, Raipur, Chhattisgarh, India
Ananta Choudhury
Department of Pharmaceutics, Assam Down Town University, Assam, India

Abstract


This study intended to explore the influence of formulation factors on the physico-chemical properties of quercetin-loaded liposomes and optimize the fabrication environment. Thin film hydration technique was employed to prepare liposome and optimization was done by 32 factorial designs combined with desirability function. Nine preparations were prepared by using altered drug: lipid and soyphosphatidylcholine: cholesterol (SPC: cholesterol) ratios and assessed for entrapment efficacy and vesicle size. The findings were the mean diameter and drug encapsulation efficiency. Results exhibited that SPC concentration and SPC: cholesterol molar ratio had a solid impact on liposome size. Increasing the lipid ratio produced a reduction in size. The degree of quercetin charging depended on the factors evaluated. Increasing SPC concentration and lipid ratio pointed lyboosted quercetin entrapment. However, higher quercetin concentrations had a negative effect on drug entrapment. Based on this, an optimized design was determined, prepared and investigated. The entrapment efficiency and vesicle size were found to be very adjacent with the predicted values. The formulation was found to be globular shape and also shows sustained release pattern. These results backing the fact that 32 full factorial designs with desirability function might be efficiently used in optimization of quercetin loaded liposome. The overall results showed that SPC concentration and lipid ratio were the key features influencing particle size, while entrapment efficiency was affected primarily by quercetin concentration.

Keywords


Fabrication, In-Vitro Evaluation, Liposomal Quercetin.

References