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Isolation and Screening the Pharmacological Activities of Vegetative and Spore-Crystal Proteins from Bacillus thuringiensis
Bacillus thuringiensis, the most successful and most widely used microbial insecticide, produces crystal proteins. The physiological significance of the crystal proteins poorly understood except for the potent insecticidal activity. In the current study, an attempt made to isolate vegetative and spore-crystal proteins from Bacillus thuringiensis NCIM2514 and to perform their hemolytic and antioxidant activities. The proteins were separated by salting out methods then by using SDS-PAGE. The molecular protein weight of the vegetative and spore-crystal protein identified as the presence of low molecular weight protein. Pharmacological activities as antioxidant and hemolytic studies were performed for the crude proteins and found as Spore-crystal proteins contain more anti-oxidant when compared to vegetative proteins.
Bacillus thuringiensis (Bt), Spore-Crystal Proteins, Vegetative Proteins. SDS-PAGE, Antioxidant.
- Akiba T, Abe Y, Ktada S, Kusaka Y, Ito A, Ichimatsu T, Katayaman H, Akao T, Higuchi K, Mizuki E, Ohba M, Kanai R and Harata K. Crystal structure of the parasporin-2 Bacillus thuringiensis toxin that recognizes cancer cells. Journal of Molecular Biology. 2009; 386 (1): 121-33.
- Aronson AI and Shai Y. Why Bacillus thuringiensis insecticidal toxins are so effective: unique features of their mode of action. FEMS Microbiology Letters. 2001; 195: 1-8.
- Baum JA, Johnson TB and Carlton BC. Bacillus thuringiensis: Natural and recombinant bioinsecticide products. In: Biopesticides: Use and Delivery, Hall FR and Menn JJ, Eds, Humana Press 1999, Totowa, NJ, 189-210.
- Becker N. Bacterial control of vector mosquitoes and black flies In: Entomopathogenic Bacteria: From Laboratory to Field Application. Charles JF, Delecluse A, Neilson-LeRoux (Eds). Dordrecht, Kluwer Academic Publishers 2000; 383-98.
- Capello M, Bungiro RD, Harrison LM, Bischof LT, Griffitts JS, Barrows BD and Aroian RV. A purified Bacillus thuringiensis crystal protein with therapeutic activity against the hookworm parasite Ancylostomaceylanicus. Proc Natl AcadSci USA 2006; 103: 15154-9.
- Crickmore N, Zeigler DR, Feitelson J, Schnepf E, Van Rie J, Lereclus D, Baum J and Dean DH. Revision of the literature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiology Molecular Biology Review 1998; 62: 807-13.
- Jung YC, Mizuki E, Akao T and Cote JC. Isolation and characterization of a novel Bacillus thuringiensis strain expressing a novel crystal protein with cytocidal activity against human cancer cells. Journal of Applied Microbiology. 2007; 103: 65-79.
- Katayama H, Kusaka Y, Yokota H, Akao T, Kojima M, Nakamura O, Mekada E and Mizuki E. Parasporin-1, a novel cytotoxic protein from Bacillus thuringiensis, induces Ca2+ influx and a sustained elevation of the cytoplasmic Ca2+ concentration in toxin-sensitive cells. Journal of Biological Chemistry. 2007; 282: 7742-52.
- Kitada S, Abe Y, Shimada H, Kusaka Y, Matsuo Y, Katayama H, Okumura S, Akao T, Mizuki E, Kuge O, Sasaguri Y, Ohba M and Ito A. Cytocidal actions of parasporin2, an anti-tumour crystal toxin from Bacillus thuringiensis. Journal of Biological Chemistry 2006; 281(36): 26350-60.
- Mizuki E, Ohba M, Akao T, Yamashita S, Saitoh H and Park YS. Unique activity associated with non-insecticidal Bacillus thuringiensis parasporal inclusions: in vitro cell-killing action on human cancer cells. Journal of Applied Microbiology. 1999; 86: 477-86.
- Mizuki E, Park YS, Saitoh H, Yamashita S, Akao T, Higuchi K and Ohba M. Parasporin, a human leukaemic cell-recognizing parasporal protein of Bacillus thuringiensis. Clinical and Diagnostic Laboratory Immunology. 2000; 7: 625-34.
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