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Isolation and Screening the Pharmacological Activities of Vegetative and Spore-Crystal Proteins from Bacillus thuringiensis


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1 Department of Pharmaceutics, SRM College of Pharmacy, SRM University, Kattankulathur, Tamil Nadu, India
     

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Bacillus thuringiensis, the most successful and most widely used microbial insecticide, produces crystal proteins. The physiological significance of the crystal proteins poorly understood except for the potent insecticidal activity. In the current study, an attempt made to isolate vegetative and spore-crystal proteins from Bacillus thuringiensis NCIM2514 and to perform their hemolytic and antioxidant activities. The proteins were separated by salting out methods then by using SDS-PAGE. The molecular protein weight of the vegetative and spore-crystal protein identified as the presence of low molecular weight protein. Pharmacological activities as antioxidant and hemolytic studies were performed for the crude proteins and found as Spore-crystal proteins contain more anti-oxidant when compared to vegetative proteins.

Keywords

Bacillus thuringiensis (Bt), Spore-Crystal Proteins, Vegetative Proteins. SDS-PAGE, Antioxidant.
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  • Isolation and Screening the Pharmacological Activities of Vegetative and Spore-Crystal Proteins from Bacillus thuringiensis

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Authors

R. Kavitha
Department of Pharmaceutics, SRM College of Pharmacy, SRM University, Kattankulathur, Tamil Nadu, India
N. Damodharan
Department of Pharmaceutics, SRM College of Pharmacy, SRM University, Kattankulathur, Tamil Nadu, India
S. Sangeetha
Department of Pharmaceutics, SRM College of Pharmacy, SRM University, Kattankulathur, Tamil Nadu, India
P. N. Remya
Department of Pharmaceutics, SRM College of Pharmacy, SRM University, Kattankulathur, Tamil Nadu, India
T. S. Saraswathi
Department of Pharmaceutics, SRM College of Pharmacy, SRM University, Kattankulathur, Tamil Nadu, India

Abstract


Bacillus thuringiensis, the most successful and most widely used microbial insecticide, produces crystal proteins. The physiological significance of the crystal proteins poorly understood except for the potent insecticidal activity. In the current study, an attempt made to isolate vegetative and spore-crystal proteins from Bacillus thuringiensis NCIM2514 and to perform their hemolytic and antioxidant activities. The proteins were separated by salting out methods then by using SDS-PAGE. The molecular protein weight of the vegetative and spore-crystal protein identified as the presence of low molecular weight protein. Pharmacological activities as antioxidant and hemolytic studies were performed for the crude proteins and found as Spore-crystal proteins contain more anti-oxidant when compared to vegetative proteins.

Keywords


Bacillus thuringiensis (Bt), Spore-Crystal Proteins, Vegetative Proteins. SDS-PAGE, Antioxidant.

References