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Molecular Detection of Pathogenic Entamoeba histolytica based on Amoebapore C Gene in Fecal Samples of Camels from Babylon Province, Iraq


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1 Lecturer, Branch of Parasitology, Collage of Veterinary Medicine, University of Al-Qasim Green, Iraq
     

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The attention in the current study was payed to the identification of pathogenic Entamoeba histolytica (PEH) using molecular techniques targetedtheamoebapore C (AP-C) gene in fecal samples of camels from Babylon province, Iraq. Fifty, 50 camels, fecal samples were collected and analyzed in a laboratory using a real-time polymerase chain reaction (RT-PCR) method to detect the amplification of the AP-C gene. The results demonstrated that 28 (56%) out of 50 samples were positive for the presence of the AP-C gene. The findings showed high efficiency rate (99.7%) of the current used technique in detecting the gene in the fecal samples of camels.The present work provides interesting data about the current presence of the pathogenic Entamoeba histolytica in the feces of the tested camels from Babylon Province, Iraq.

Keywords

Amoebapore C, camels, Feces, Pathogenic Entamoeba histolytica, RT-qPCR.
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  • Molecular Detection of Pathogenic Entamoeba histolytica based on Amoebapore C Gene in Fecal Samples of Camels from Babylon Province, Iraq

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Authors

Qassim Jawad Ameer
Lecturer, Branch of Parasitology, Collage of Veterinary Medicine, University of Al-Qasim Green, Iraq
Nawras Abdul Bari Madlol
Lecturer, Branch of Parasitology, Collage of Veterinary Medicine, University of Al-Qasim Green, Iraq

Abstract


The attention in the current study was payed to the identification of pathogenic Entamoeba histolytica (PEH) using molecular techniques targetedtheamoebapore C (AP-C) gene in fecal samples of camels from Babylon province, Iraq. Fifty, 50 camels, fecal samples were collected and analyzed in a laboratory using a real-time polymerase chain reaction (RT-PCR) method to detect the amplification of the AP-C gene. The results demonstrated that 28 (56%) out of 50 samples were positive for the presence of the AP-C gene. The findings showed high efficiency rate (99.7%) of the current used technique in detecting the gene in the fecal samples of camels.The present work provides interesting data about the current presence of the pathogenic Entamoeba histolytica in the feces of the tested camels from Babylon Province, Iraq.

Keywords


Amoebapore C, camels, Feces, Pathogenic Entamoeba histolytica, RT-qPCR.



DOI: https://doi.org/10.37506/v20%2Fi1%2F2020%2Fmlu%2F194458