Determination Enterococcus faecalis in Asymptomatic Urinary Tract Infection Associated with Diabetes Type 2 Patients in Suwayrah General Hospital-Iraq

Background: Asymptomatic Urinary Tract Infection is common in diabetes type-2 patient and predisposes these patients to more severe urinary tract infections (UTIs). Enterococci have been documented to cause infection of the urinary tract and other sites. Although several species of Enterococcus have been recognized, Enterococcus faecalis has been considered one of the major agents of urinary tract infections. Objectives: To determine the percentage Enterococcus faecalis of ASB , antimicrobial resistance in diabetics and non-diabetics in urine sample and detection of enterococcal surface protein (esp) gene . Method: The study involved a total of 611 participants including 197 diabetes mellitus patients and 414 non-diabetics in Suwayrah-Wasit-Iraq. Mid-stream urine was collected from consented subjects and dipstick , general urine examination and bacterial culture were conducted for each sample. Isolates were identified using standard biochemical tests and VITEK-2 system . Results: All collected samples are asymptomatic bacteriuria. There was a significant difference in the percentage of asymptomatic bacteriuria (ASB) between diabetics and non-diabetics (P = 0.010). There was no significant difference in resistance between diabetics and non-diabetics (P > 0.05). Enterococcus faecalis isolates showed (50%) resistance to Erythromycin and Levofloxacin. The esp gene was observed in all Enterococcus faecalis isolates . Conclusion: The females of age more than 60 years old have the higher percentage of asymptomatic UTI. Erythromycin and Levofloxacin were more resistance in Enterococci isolated