Refine your search
Collections
Co-Authors
Journals
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Iliger, Sudhir R.
- Isolation, Characterization and Evaluation of Endophytic Fractions of Centella asiatica Linn. (Leaves) for Invitro Antioxidant Activity
Abstract Views :308 |
PDF Views:219
Authors
Affiliations
1 Department of Pharmacognosy, Post Graduate Studies and Research Center, S.E.T’s College of Pharmacy, S. R. Nagar, Dharwad - 580002, Karnataka, IN
2 Department of Pharmaceutics, S.E.T's College of Pharmacy, S.R. Nagar, Dharwad - 580002, Karnataka, IN
1 Department of Pharmacognosy, Post Graduate Studies and Research Center, S.E.T’s College of Pharmacy, S. R. Nagar, Dharwad - 580002, Karnataka, IN
2 Department of Pharmaceutics, S.E.T's College of Pharmacy, S.R. Nagar, Dharwad - 580002, Karnataka, IN
Source
Journal of Natural Remedies, Vol 20, No 1 (2020), Pagination: 29-41Abstract
In the present investigation, we have carried out the isolation of fungal endophytes from Centella asiatica Linn leaves followed by fermentation and extraction of fungal endophytes with non-polar solvents such as chloroform, ethyl acetate and n-butanol. Preliminary phytochemical investigation of endophytic crude fractions of leaves were also determined to detect the presence of primary and secondary metabolites followed by invitro free radical scavenging activity by reducing power, DPPH and hydroxyl radical assay. The chloroform fungal endophytic fractions were subjected to column chromatography by gradient elution technique for isolation of possible secondary metabolite. Reducing power of endophytic extracts of C. asiatica Leaf (CAL-1) (50-450 μg/ml) increased with increase in concentration. Reaction with DPPH radicals of CAL-1 showed good scavenging activity. The IC50 values for Ascorbic acid, chloroform extract, ethyl acetate extract and n-butanol extract were found to be 30.33 μg/ml, 66.58 μg/ml, 79.33 μg/ml and 96.39 μg/ml respectively. In hydroxyl radical assay, The IC50 values for mannitol, chloroform extract, ethyl acetate extract and n-butanol extract were found to be 121.06 μg/ml, 141.21 μg/ml, 181.80 μg/ml and 189.90 μg/ml respectively. The endophytic crude fractions of ethyl acetate exhibited potent antioxidant activity as compared to other fractions. Hence, ethyl acetate fungal endophytic fractions of Centella asiatica Linn leaves can be employed as a potential antioxidant in the prevention of oxidative stress caused by the free radicals.Keywords
Antioxidant, Centella asiatica, Column Chromatography, Endophyte.Full Text
References
- Kunkel BA, Grewal PS, Quigley MF. A mechanism of acquired resistance against an entomopathogenic nematode by Agrotis ipsilon feeding on perennial ryegrass harboring a fungal endophyte. Biological Control. 2004; 29(1):100-08. https://doi.org/10.1016/S1049-9644(03)00119-1.
- Shen L, Ye YH, Wang XT, Zhu HL, Xu C, Song YC, Li H, Tan RX. Structure and total = synthesis of aspernigerin: a novel cytotoxic endophyte metabolite. Chemistry-A European Journal. 2006; 12(16):4393-96. https://doi.org/10.1002/chem.200501423. PMid:16555343.
- Strobel GA. Rainforest endophytes and bioactive products. Critical Reviews in Biotechnology. 2002; 22(4):315-33. https://doi.org/10.1080/07388550290789531. PMid:12487423.
- Garai S, Mahato SB, Ohtani K, Yamasaki K. Bacopasaponin D-A Pseudojujubogenin glycoside from Bacopa monniera. Phytochemistry. 1996; 43(2):447. https://doi.org/10.1016/0031-9422(96)00250-6.
- Singh HK. and Dhavan B.N. Preclinical Neuropsycho pharmacological Investigations on Bacosides: A Nootropic Memory Enhancer (Abs. T-3: 3),Central Drug Reasearch Institute, Luknow, 1996, P-65.
- Zheng CJ, Qin LP. Chemical components of Centella asiatica and their bioactivities. Phytochemistry. 2007; 5(3):348-51. https://doi.org/10.3736/jcim20070324. PMid:17498500.
- Devi NN, Prabakaran JJ. Bioactive metabolites from an endophytic fungus Penicillium sp. isolated from Centella asiatica. Curr. Res. Environ Appl. Mycol. 2014; 4(1):34-43. https://doi.org/10.5943/cream/4/1/3.
- Gawas P, Shenoy BD, Hyde KD, Bhat DJ. Echinosphaeria macrospora sp. nov. Teleomorph of Vermiculariopsiella Endophytica sp. nov. 2006; 27:11-20.
- Krishnamurthy YL, Naik SB, Jayaram S. Fungal communities in herbaceous medicinal plants from the Malnad region, Southern India. Microbes and Environments. 2008; 23(1):2448. https://doi.org/10.1264/jsme2.23.24. PMid:21558683.
- Satheesan J, Narayanan AK, Sakunthala M. Induction of ischolar_main colonization by Piriformospora indica leads to enhanced asiaticoside production in Centella asiatica. Mycorrhiza. 2012; 22(3):195-202. https://doi.org/10.1007/s00572-011-0394-y. PMid:21688071.
- Rafat A, Philip K, Muniandy S. A novel source of bioactive compounds: endophytic bacteria isolated from Centella asiatica. J. Pure. Appl. Microbiol. 2012; 6(1):11-20.
- Rakotoniriana EF, Rafamantanana M, Randriamampionona D, Rabemanantsoa C, Urveg-Ratsimamanga S, El Jaziri M, Munaut F, Corbisier AM, Quetin-Leclercq J, Declerck S. Study in vitro of the impact of endophytic bacteria isolated from Centella asiatica on the disease incidence caused by the hemibiotrophic fungus Colletotrichum higginsianum. Antonie Van Leeuwenhoek. 2013; 103(1):121-33. https://doi.org/10.1007/s10482-012-9791-2. PMid:22903452.
- Nath A, Pathak J, Joshi SR. Bioactivity assessment of endophytic fungi associated with Centella asiatica and Murraya koengii. Journal of Applied Biology and Biotechnology. 2014; 2(05):006-11.
- Prabakaran NN, Femina W. Antibiogram pattern of enhopityc fungi isolated from medical plant Centella asiatica. Journal of Pharmacy Research. 2012; 5:205-07.
- Veerendra Kumar MH, Gupta YK. Effect of Centella asiatica on cognition and oxidative stress in an intracerebroventricular streptozotocin model of Alzheimer’s disease in rats. Clinical and Experimental Pharmacology and Physiology. 2003; 30(5):336-42. https://doi.org/10.1046/ j.1440-1681.2003.03842.x. PMid:12859423.
- Xu Y, Cao Z, Khan I, Luo Y. Gotu Kola (Centella asiatica) extract enhances phosphorylation of cyclic AMP response element binding protein in neuroblastoma cells expressing amyloid beta peptide. Journal of Alzheimer’s Disease. 2008; 13(3):341-49. https://doi.org/10.3233/JAD-2008-13311. PMid:18431001.
- Jayashree G, Muraleedhara GK, Sudarslal S, Jacob VB. Anti-oxidant activity of Centella asiatica on lymphomabearing mice. Fitoterapia. 2003; 74(5):431-34. https://doi.org/10.1016/S0367-326X(03)00121-7.
- Ernawati M, Solihin DD, Lestari YU. Community structures of endophytic actinobacteria from medicinal plant Centella asiatica L. urban-based on metagenomic approach. Int. J. Pharm Pharm Sci. 2016; 8:292-97.
- Nongkhlaw FM, Joshi SR. Micrographical assessment of antifungal effect of endophytic bacteria. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 2016; 86(1):9-14. https://doi.org/10.1007/s40011014-0321-z.
- Heidari M, Jamshedi AH, Akhondzadeh SH, Ghaffari NM, Sadeghi MR, Khansari GM, et al. Evaluating the effects of Centella asiatica on spermatogenesis in rats. Med. J. Reprod. Infertility. 2007; 7:367-74.
- Wiyakrutta S, Sriubolmas N, Panphut W, Thongon N, Danwisetkanjana K, Ruangrungsi N, Meevootisom V. Endophytic fungi with anti-microbial, anti-cancer and antimalarial activities isolated from Thai medicinal plants. World journal of microbiology and biotechnology. 2004; 20(3):26572. https://doi.org/10.1023/B:WIBI.0000023832.27679.a8.
- Silva GH, Teles HL, Trevisan HC, Bolzani VD, Young M, Pfenning LH, Eberlin MN, Haddad R, Costa-Neto CM, Araújo ÂR. New bioactive metabolites produced by Phomopsis cassiae, an endophytic fungus in Cassia spectabilis. Journal of the Brazilian Chemical Society. 2005; 16(6B):146366. https://doi.org/10.1590/S0103-50532005000800029.
- Tejasvi MV, Kini KR, Prakash HS, Subbiah V, Shetty HS. Antioxidant, antihypertensive and antibacterial properties of endophytic pestalotiopsis species from medicinal plants. Can. J. Microbiol. 2008; 54:769-80. https://doi.org/10.1139/ W08-070. PMid:18772940.
- Shukla ST, Kulkarni VH, Habbu PV, Jagadeesh KS, Patil BS, Smita DM. Hepatoprotective and antioxidant activities of crude fractions of endophytic fungi of Ocimum sanctum Linn. in rats. Oriental Pharmacy and Experimental Medicine. 2012; 12(2):81-91. https://doi.org/10.1007/s13596-012-00617.
- Doty SL, Dosher MR, Singleton GL, Moore AL, Van Aken B, Stettler RF, Strand SE, Gordon MP. Identification of an endophytic Rhizobium in stems of Populus. Symbiosis. 2005; 39(1):27-35.
- Sharma BJ. Thin layer chromatography, 2nd Edn., INC, New York; 1986. p. 119.
- Staul E. Thin layer chromatography, 2nd Edn., Heidelburg, Newyork; 1990. p. 362.
- Wagner H, Bladet S. Plant drug analysis, Heidelburg, 2nd Edn., Newyork; 1994. p. 192.
- Doty SL, Dosher MR, Singleton GL, Moore AL, Van Aken B, Stettler RF, Strand SE, Gordon MP. Identification of an endophytic Rhizobium in stems of Populus. Symbiosis. 2005; 39(1):27-35.
- Wiyakrutta S, Sriubolmas N, Panphut W, Thongon N, Danwisetkanjana K, Ruangrungsi N, Meevootisom V. Endophytic fungi with anti-microbial, anti-cancer and antimalarial activities isolated from Thai medicinal plants. World Journal of Microbiology and Biotechnology. 2004; 20(3):26572. https://doi.org/10.1023/B:WIBI.0000023832.27679.a8.
- Silva GH, Teles HL, Trevisan HC, Bolzani VD, Young M, Pfenning LH, Eberlin MN, Haddad R, Costa-Neto CM, Araújo ÂR. New bioactive metabolites produced by Phomopsis cassiae, an endophytic fungus in Cassia spectabilis. Journal of the Brazilian Chemical Society. 2005; 16(6B):146366. https://doi.org/10.1590/S0103-50532005000800029.
- Tejasvi MV, Kini KR, Prakash HS, Subbiah V, Shetty HS. Antioxidant, antihypertensive and antibacterial properties of endophytic pestalotiopsis species from medicinal plants. Can J. Microbiol. 2008; 54:769-80. https://doi.org/10.1139/W08070. PMid:18772940.
- Shukla ST, Kulkarni VH, Habbu PV, Jagadeesh KS, Patil BS, Smita DM. Hepatoprotective and antioxidant activities of crude fractions of endophytic fungi of Ocimum sanctum Linn. in rats. Oriental Pharmacy and Experimental Medicine. 2012; 12(2):81-91. https://doi.org/10.1007/s13596-012-0061-7.
- Kashmira JG, Jagruti AP and Anuradha KG. Pharmacological review on Centella asiatica: A potential herbal cure. Indian. J. Pharma Sci. 2010; 75(5): 546-56. https://doi.org/10.4103/0250474X.78519. PMid:21694984, PMCid:PMC3116297.
- Choudary N, Bijem KR, Kalia AN. Antiepileptic potential of flavonoids fraction from the leaves of Anisomeles malabarica. Journal of Ethanopharmacology, 2011; 135(2):238-42. https://doi.org/10.1016/j.jep.2011.02.019. PMid:21354295.
- Shastry RA, Smita D. Madagundi, Prasanna V. Habbu et al. Phytochemical investigation and antiepileptic activity of Aspergus racemosus(Wild) ischolar_main extracts in rodents. RGUHS J. Pharm Sci. 2015; 5(3):97-103. https://doi.org/10.5530/rjps.2015.3.3.