Journal of Natural Remedies

S. S. Jalalpure , M. B. Patil , K. R. Alagawadi

Abstract

Objective: To screen the wound healing activity of different extracts of galls of Quercus infectoria on incision, excision and dead space (granulation) wound models in albino rats. Materials and method: Crude aqueous extract of Quercus infectoria galls and its three different crude fractions, petroleum ether (40°-60°C), solvent ether, ethyl acetate were tested for various preliminary phytoconstituents and were screened at dose of 100mg/kg for wound healing properties in incision, excision and dead space (granulation) wound models in albino rats. Results: Tannins, flavonoids, steroids and carbohydrates were found to be present in aqueous extract and its various fractions of galls of Quercus infectoria. In resutured incision wound models aqueous extract and its fractions showed significant (P<0.01) breaking strength compared to control. In excision wound parameters the aqueous extract promotes better wound healing (96.36%) compared to control and organic fractions. Breaking strength of the grass pith induced granuloma studies the results show significant (P<0.01) activity in case of aqueous extract along with solvent ether fraction. Conclusion: From the results obtained, it can be observed that aqueous extract and its fractions of galls of Quercus infectoria have significant wound healing property. Also it can be concluded that flavonoids may be responsible for wound healing activity.

Keywords

Quercus infectoria, Wound-healing, Aqueous Extract, Soxhlet Extraction

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S. S. Jalalpure , M. B. Patil , M. S. Jabshetti

Abstract

Objectives: To screen the hepatoprotective activity of leaves of Lawsonia alba against carbon tetrachloride induced hepatotoxicity in albino rats. Method: Studies on the hepatoprotective activity of the leaves of Lawsonia alba were carried out in albino rats. The Lawsonia alba leaf ethanolic extract and three different fractions obtained from it were screened for hepatoprotective activity using carbon tetrachloride-induced hepatic damage model in rats. Doses for the different fractions and ethanolic extract were selected based on the results of acute toxicity studies in mice. The effect was assessed by serum enzyme profile, viz., glutamic oxaloacetate transaminase (GOT) and glutamic pyruvate transaminase (GPT) levels, and histopathological changes in liver. Results: Lawsonia alba ethanolic extract significantly protected from biochemical and histological changes induced by carbontetrachloride in rats. Conclusion: Ethanolic extract of Lawsonia alba leaves exhibited a significant protection against carbon tetrachloride-induced hepatic damage in rats.

Keywords

Carbon Tetrachloride, Hepatoprotective Activity, Leaves of Lawsonia alba Lam. (Lythraceae), Liv.52 Syrup, Olive Oil, 5% Gum Acacia, SGPOT, SGPT

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S. S. Jalalpure , Shivaji Bamne , M. B. Patil , Biren Shah , Md. Salahuddin

Abstract

Objective: To screen the antidiabetic potential of the bark of Holarrhena antidysenterica Linn. Materials and methods: In the present study, bark of Holarrhena antidysenterica Linn was screened for antidiabetic activity. Bark powder of H.antidysenterica was subjected to hot continuous extraction (soxhlet) with various solvents like petroleum ether (40-60°C), chloroform, butanol, butanone and alcohol. Aqueous extract was prepared by cold maceration. After preliminary phytochemical investigation, all the extracts were evaluated for antidiabetic activity after single dose (acute study) and after prolonged treatment (chronic study) in alloxan induced diabetic albino rats. All the extracts were given orally at a dose of 250 mg/kg b.w., Glibenclamide was used as standard drug (10 mg/kg b.w. p.o.). Results and discussion: Alcohol, butanol, chloroform, aqueous and butanone showed significant antidiabetic activity in acute as well as prolonged treatment compared to control. Petroleum ether extract did not show significant antidiabetic activity on prolonged treatment. Among all the extracts, alcoholic extract had more significantly reduced the blood glucose level after single dose and nearly equal to standard Glibenclamide after prolonged treatment.

Keywords

Holarrhena antidysenterica, Antidiabetic, Glibenclamide, Alloxan

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Maheshwar G. Hogade , S. S. Jalalpure , Somnath D. Bhinge , Sonali Kuthar , S. S. Kosgi

Abstract

The present study was undertaken to evaluate the anthelmintic activity of aqueous and ethanolic extracts of the bark of Azadirachta indica (Family - Meliaceae) and its different extracts (viz. ethanol and aqueous) against Ascardi galli and Eudrilus eugeniae. Various concentrations (50 and 100 mg/ml) of ethanolic and aqueous extracts and its various fractions were evaluated in the bioassay involving determination of time of paralysis (P) and time of death (D) of both types of the worms. Piperazine citrate was used as standard anthelmintic drug and distilled water was used as control. The results of the present study indicated that the alcoholic ethanol and aqueous extracts of the bark significantly exhibited paralysis (P < 0.05) in worms in lower dose 50 mg/ml and also caused death of worms especially at higher concentration of 100 mg/ml, as compared to standard drug. Further studies are in process to isolate the active principle's responsible for the activity.

Keywords

Azadirachta indica Bark, Anthelmintic Activity, Ascardi galli and Eudrilus eugeniae, Piperazine Citrate.

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