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Vyas, Niraj
- Assessment of Testosterone Biogenesis Enhancement by a Lupeol Derivative Separated from Seeds of Hygrophila spinosa T. Ander in vitro
Authors
1 Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, IN
2 Department of Pharmaceutical Chemistry and Analysis, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, IN
Source
Journal of Natural Remedies, Vol 21, No 4 (2021), Pagination: 317-322Abstract
The present study aimed towards assessing the isolated lupeol derivative in vitro for testosterone biogenesis enhancement by the action on isolated rat leydig cells. Studies were carried out to separate a novel bioactive molecule from an Indian medicinal plant, Hygrophila spinosa, used as an aphrodisiac and spermatogenic for years. Unsaponifiable bioactive fraction, prepared from seeds of the plant, was subjected to isolation of phytoconstituents by column and flash chromatography. The isolated compound was characterized by spectral studies as lupeol methyl ether. Later, it was subjected to in vitro studies using isolated leydig cells from rat for its action. Different amounts of isolated compound were incubated with leydig cells for 3 hours and concentration of testosterone after incubation was considered as a parameter. Dehydroepiandrosterone was used as positive control in the studies. The results indicated that the isolated compound was active and enhanced the testosterone biogenesis, as evident from increased amount of testosterone found in a medium treated with compound. This was the first report included isolation, chemical characterization and biological activity of Lupeol methyl ether from Hygrophila spinosa T. Ander.Keywords
Hygrophila spinosa, Lupeol, Leydig Cells, Steroids, Tri-terpenoids, Testosterone.References
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- Urolithiasis: HPTLC Method for Quantitative Detection of Rutin and Quercetin in an Herbal Plant
Authors
1 Department of Pharmacology, Indukaka Ipcowala College of Pharmacy, New Vallabh Vidyanagar, Anand - 388121, Gujarat, IN
2 Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Changa, Anand - 388421, Gujarat, IN
3 Department of Pharmacology, L.M. College of Pharmacy, Ahmedabad - 380058, Gujarat, IN
Source
Journal of Natural Remedies, Vol 22, No 3 (2022), Pagination: 371 - 379Abstract
Anti-inflammatory, Hypoglycemic, Hepatoprotective, Antihyperlipidemic, Anti-Ulcerative, Cardioprotective Stimulant, Sedative, Hypnotic, Anticonvulsant Activity, Memory Retention, Stroke Preventive Activity, Antimicrobial Activity, Antimycobacterial, Antiviral, Larvicidal, Antiparasitic, Chemopreventative, Chemo-modulatory, Anticancer, Cytoprotective activities were found in a variety of herbal plants, including Ocimum basilicum. The purpose of this study was to design and develop a new HPTLC method that was accurate, precise and cost-effective for simultaneous measurement of rutin and quercetin in a hydroalcoholic extract of Ocimum basilicum seeds. The mobile phase was Toluene: Ethyl Acetate: Methanol: formic acid (6:4:3:1, v/v/v/v/v) and densitometric scanning was performed at 254 nm. Merck TLC aluminium sheets of silica gel 60 F254, (10 x 10 cm) with a thickness of 250 mm was used as stationary phase. At a wavelength of 254 nm, rutin and quercetin were detected. The constituents were resolved satisfactorily, with Rf values of 0.25 ± 2.01 for rutin and 0.80 ± 0.64 for quercetin, respectively. Linearity (300-1300 ng/spot for rutin and quercetin) was used to test the method’s accuracy and reproducibility. For both analytes, intra- and inter-day precision, as evaluated by coefficient of variation, was less than 3%. For rutin, the detection and quantification limits were 46.52 and 140.96 ng/spot, respectively, and for quercetin, they were 81.79 and 247.84 ng/spot. The proposed approach was found to be precise, accurate, repeatable, and specific, and it could be used to measure quercetin and rutin in samples at the same time.
Keywords
Densitometry, Ocimum basilicum, Quercetin, High-performance Thin-layer Chromatography (HPTLC), Rutin, Validation.References
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