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Aziz, Mohammed
- Antithrombotic, Antiaggregant and Anticoagulant Effect of Methanolic Fraction of Calamintha officinalis: In vitro and ex vivo Experiments
Abstract Views :291 |
PDF Views:168
Authors
Kaoutar Zoheir
1,
Mohamed Bnouham
1,
Abdelkhaleq Legssyer
1,
Abderrahim Ziyyat
1,
Mohammed Berrabah
2,
Mohammed Aziz
1,
Mohammed Bensaid
3,
Hassane Mekhfi
1
Affiliations
1 Laboratory of Physiology, Genetic and Ethnopharmacology, University Mohammed the First, Oujda, 60000, MA
2 Laboratory of Mineral Solid and Analytical Chemistry, Department of Chemistry, University Mohammed the First, Oujda 60000, MA
3 Laboratory Bensaid of Pathological Cytology and Anatomy, 100, Allal Ben Abdellah Street, Apartment number 9, Oujda, 60000, MA
1 Laboratory of Physiology, Genetic and Ethnopharmacology, University Mohammed the First, Oujda, 60000, MA
2 Laboratory of Mineral Solid and Analytical Chemistry, Department of Chemistry, University Mohammed the First, Oujda 60000, MA
3 Laboratory Bensaid of Pathological Cytology and Anatomy, 100, Allal Ben Abdellah Street, Apartment number 9, Oujda, 60000, MA
Source
Journal of Natural Remedies, Vol 18, No 4 (2018), Pagination: 131-142Abstract
The objective was to study the effect of Calamintha officinalis methanolic fraction in the prevention of thrombosis and to explore its effect on primary and secondary hemostasis. The antithrombotic activity was evaluated by using the pulmonary thromboembolism experimental model. Platelet aggregation, tail bleeding time and coagulation assays were explored to elucidate the antithrombotic mechanism. For aggregation, washed platelets were incubated in presence of Calamintha officinalis methanolic fraction and then activated by different agonists (thrombin, ADP, collagen). The bleeding time was determined to measure the time between the tail incision and the moment the bleeding stops. The coagulation was explored by measuring prothrombin time, activated partial thromboplastin time, thrombin time and plasmatic fibrinogen concentration. For ex vivo study, the animals were treated by the Calamintha officinalis methanolic fraction (250 mg/kg/day) for 4 weeks. Then, platelet aggregation, tail bleeding time and coagulation parameters was studied. The results indicate that C. officinalis methanol fraction seems to exert in vivo an antithrombotic action (40% of protection). The in vitro investigations also show that this fraction significantly inhibits aggregation, increases bleeding time, coagulation times and reduces fibrinogen concentration. The chronic treatment of animals confirms the precedent data. This study demonstrates an antithrombotic effect of C. officinalis. The observed antiaggregant and anticoagulant effects could explain this activity.Keywords
Bleeding, Calamintha officinalis, Coagulation, Platelet Aggregation, Thrombosis.Full Text
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- Relaxant and Antispasmodic Activities of Aqueous Extract from Thymus algeriensis Boiss. and Reut.
Abstract Views :200 |
PDF Views:131
Authors
Leila Beyi
1,
Hassan Zrouri
1,
Hanane Makrane
1,
Hassane Mekhfi
1,
Abderrahim Ziyyat
1,
Mohamed Bnouham
1,
Mohammed Aziz
1
Affiliations
1 Laboratory of Bioressources, Biotechnologies, Ethnopharmacologie et Santé, Faculty of Sciences, Mohamed the First University, PB 717, 60000, Oujda, MA
1 Laboratory of Bioressources, Biotechnologies, Ethnopharmacologie et Santé, Faculty of Sciences, Mohamed the First University, PB 717, 60000, Oujda, MA
Source
Journal of Natural Remedies, Vol 21, No 2 (2021), Pagination: 165-171Abstract
Thymus algeriensis have been vastly utilized for intestinal disorders. The purpose of this investigation was to scrutinize the probable mechanism for its utilization in the spasm disorder. Aqueous extract of this medicinal plant (AqTA) was tested in vitro on rat and rabbit jejunum. The extract produced relaxation of rabbit jejunum. This relaxation does not depend on the adrenergic pathway, the AqTA induces inhibition irrespective of the presence or absence of adrenergic inhibitors. AqTA engendered a concentration-dependent (0.1-5 mg/ml) relaxation of carbamylcholine chloride (CCh) and K+ provoked tones in rat intestine with IC50 values of 2.06 ± 0.26 and 3.55 ± 0.48 (mg/ml) respectively. This extract likewise induced a dosedependent (0.1-3 mg/ml) rightward shift in the CCh and Ca++ dose-response curves. The AqTA alone has decreased more significantly the percentage of contraction of rat jejunum than the AqTA pre-incubated with atropine or hexamethonium then contracted with KCl; but there is no significant difference by those pre-incubated with methylene blue or L-NAME. When the intestine was pretreated with nifedipine and contracted by CCh, the antispasmodic effect provoked by AqTA with and without pre-incubation with nifedipine is statistically not significant. In conclusion AqTA acts possibly on the voltage dependent Ca++ channel and cholinergic receptors but did not act on adrenergic receptors, NO and guanylatecyclase pathway. This investigation may explicate some of its traditional utilization in gut illnesses.Keywords
Antispasmodic, Jejunum, Relaxant, Smooth muscle, Thymus algeriensisFull Text