Journal of Natural Remedies

Kaoutar Zoheir ¹, Mohamed Bnouham ¹, Abdelkhaleq Legssyer ¹, Abderrahim Ziyyat ¹, Mohammed Berrabah ², Mohammed Aziz ¹, Mohammed Bensaid ³, Hassane Mekhfi ¹

Affiliations
1 Laboratory of Physiology, Genetic and Ethnopharmacology, University Mohammed the First, Oujda, 60000, MA
2 Laboratory of Mineral Solid and Analytical Chemistry, Department of Chemistry, University Mohammed the First, Oujda 60000, MA
3 Laboratory Bensaid of Pathological Cytology and Anatomy, 100, Allal Ben Abdellah Street, Apartment number 9, Oujda, 60000, MA

Abstract

The objective was to study the effect of Calamintha officinalis methanolic fraction in the prevention of thrombosis and to explore its effect on primary and secondary hemostasis. The antithrombotic activity was evaluated by using the pulmonary thromboembolism experimental model. Platelet aggregation, tail bleeding time and coagulation assays were explored to elucidate the antithrombotic mechanism. For aggregation, washed platelets were incubated in presence of Calamintha officinalis methanolic fraction and then activated by different agonists (thrombin, ADP, collagen). The bleeding time was determined to measure the time between the tail incision and the moment the bleeding stops. The coagulation was explored by measuring prothrombin time, activated partial thromboplastin time, thrombin time and plasmatic fibrinogen concentration. For ex vivo study, the animals were treated by the Calamintha officinalis methanolic fraction (250 mg/kg/day) for 4 weeks. Then, platelet aggregation, tail bleeding time and coagulation parameters was studied. The results indicate that C. officinalis methanol fraction seems to exert in vivo an antithrombotic action (40% of protection). The in vitro investigations also show that this fraction significantly inhibits aggregation, increases bleeding time, coagulation times and reduces fibrinogen concentration. The chronic treatment of animals confirms the precedent data. This study demonstrates an antithrombotic effect of C. officinalis. The observed antiaggregant and anticoagulant effects could explain this activity.

Keywords

Bleeding, Calamintha officinalis, Coagulation, Platelet Aggregation, Thrombosis.

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Leila Beyi ¹, Hassan Zrouri ¹, Hanane Makrane ¹, Hassane Mekhfi ¹, Abderrahim Ziyyat ¹, Mohamed Bnouham ¹, Mohammed Aziz ¹

Affiliations
1 Laboratory of Bioressources, Biotechnologies, Ethnopharmacologie et Santé, Faculty of Sciences, Mohamed the First University, PB 717, 60000, Oujda, MA

Abstract

Thymus algeriensis have been vastly utilized for intestinal disorders. The purpose of this investigation was to scrutinize the probable mechanism for its utilization in the spasm disorder. Aqueous extract of this medicinal plant (AqTA) was tested in vitro on rat and rabbit jejunum. The extract produced relaxation of rabbit jejunum. This relaxation does not depend on the adrenergic pathway, the AqTA induces inhibition irrespective of the presence or absence of adrenergic inhibitors. AqTA engendered a concentration-dependent (0.1-5 mg/ml) relaxation of carbamylcholine chloride (CCh) and K⁺ provoked tones in rat intestine with IC₅₀ values of 2.06 ± 0.26 and 3.55 ± 0.48 (mg/ml) respectively. This extract likewise induced a dosedependent (0.1-3 mg/ml) rightward shift in the CCh and Ca⁺⁺ dose-response curves. The AqTA alone has decreased more significantly the percentage of contraction of rat jejunum than the AqTA pre-incubated with atropine or hexamethonium then contracted with KCl; but there is no significant difference by those pre-incubated with methylene blue or L-NAME. When the intestine was pretreated with nifedipine and contracted by CCh, the antispasmodic effect provoked by AqTA with and without pre-incubation with nifedipine is statistically not significant. In conclusion AqTA acts possibly on the voltage dependent Ca⁺⁺ channel and cholinergic receptors but did not act on adrenergic receptors, NO and guanylatecyclase pathway. This investigation may explicate some of its traditional utilization in gut illnesses.

Keywords

Antispasmodic, Jejunum, Relaxant, Smooth muscle, Thymus algeriensis

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Amal Elrherabi ¹, Mohamed Bouhrim ¹, Rhizlan Abdnim ¹, Ali Berraaouan ¹, Abderrahim Ziyyat ¹, Hassane Mekhfi ¹, Abdelkhaleq Legssyer ¹, Mohamed Bnouham ¹

Affiliations
1 Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda - B.P. 717, MA

Abstract

The aqueous extract of Lavandula stoechas (AqLs) is employed as a diabetic cure in Eastern Moroccan traditional medicine. The aim of this study was to confirm and search for the antidiabetic mechanisms of this plant. The goal of this research is to look into the in vitro antioxidant activity of L. stoechas’s aqueous extract which was analyzed by using two different techniques; 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), and β-carotene bleaching assay, with an IC50 = 0.031 ± 0.02 mg/mL and an IC50 = 94.33 ± 12.5 µg/ml respectively. Furthermore, the polyphenolic and flavonoid concentrations were calculated at 146.71 ± 0.53 mg GAE/mg of AqLs, and 721,21 ± 0,21 µg QE/mg of AqLs respectively. Besides, the in vitro research of glucose consumption by Peripheral glucose consumption reveals that the combination of this extract, plus insulin, enhances the activity of insulin and improves glucose utilization by the hemidiaphragm with 166.89 ± 23.56 mg/g/h. Finally, the in vitro hemoglobin glycosylation test validated L. stoechas antidiabetic efficacy with activity equal to 48.94 ± 3.67 mg/mL compared to the gallic acid. Consequently, the aqueous extract of L. stoechas was discovered to have promising antidiabetic and antioxidant properties in this research, which can be considered for more biological exploration.

Keywords

Antidiabetic, Antioxidant, Hemidiaphragm, Glycosylation, Lavandula stoechas.

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