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Lalchhandama, K.
- Anticancer and DNA-Protecting Potentials of Spilanthes acmella (Toothache Plant) Grown in Mizoram, India
Abstract Views :366 |
PDF Views:175
Authors
Affiliations
1 Department of Life Sciences, Pachhunga University College, Aizawl – 796001, Mizoram, IN
1 Department of Life Sciences, Pachhunga University College, Aizawl – 796001, Mizoram, IN
Source
Journal of Natural Remedies, Vol 19, No 2 (2019), Pagination: 57-63Abstract
The toothache plant, Spilanthes acmella (L.) Murray, is an important culinary and medicinal plant. Here, we study its probable properties as a protective agent for DNA damage and cell proliferation, the two molecular events in oncogenesis. Plant extract was prepared by hot extraction using methanol as solvent. Various chemical tests were performed to collectively understand its free radical-scavenging activity, including 2,2-diphenyl-1-picrylhydrazyl, nitric oxide, potassium ferricyanide, hydroxyl radical, and hydrogen peroxide assays. Free radicals used or produced in these assays represent those that cause DNA damages in cells. The plant extract showed free radical-scavenging activity in all the tests and was as effective as standard drugs. Cancer cell lines such as Dalton’s lymphoma ascites (DLA) and Chinese hamster lung carcinoma (V79) cells were used for anticancer assays. The plant extract showed anticancer activity only on lymphoma cells; but there was no discernible inhibition on carcinoma. The study shows that S. acmella is a promising source of therapeutic agent in the prevention of cancer development and DNA damage.Keywords
Chinese Hamster Lung Carcinoma, Dalton’s Lymphoma Ascites, Free Radical Scavenging, Medicinal Plant, MTT Assay.Full Text
References
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- Unveiling the Unexplored and Critically Endangered Ilex khasiana for its Antioxidant Properties
Abstract Views :282 |
PDF Views:134
Authors
Charles Lalnunfela
1,
H. T. Lalremsanga
1,
T. C Lalhriatpuii
2,
David Lalnunzira
2,
K. Lalchhandama
3
Affiliations
1 Department of Zoology, Mizoram University, Tanhril – 796004, Mizoram, IN
2 Department of Pharmacy, Regional Institute of Paramedical and Nursing Sciences, Zemabawk – 796017, Mizoram, IN
3 Department of Life Sciences, Pachhunga University College, Aizawl – 796001, Mizoram, IN
1 Department of Zoology, Mizoram University, Tanhril – 796004, Mizoram, IN
2 Department of Pharmacy, Regional Institute of Paramedical and Nursing Sciences, Zemabawk – 796017, Mizoram, IN
3 Department of Life Sciences, Pachhunga University College, Aizawl – 796001, Mizoram, IN
Source
Journal of Natural Remedies, Vol 19, No 4 (2019), Pagination: 214-220Abstract
Ilex khasiana Purk. is an evergreen tree of Aquifoliaceae family reportedly endemic to Khasi Hills, Meghalaya, India; but it is also seen in a localised area of Aizawl, Mizoram, India. There is a vast history about the genus Ilex with reference to their medicinal uses in different traditional practices such as, in the treatment of coronary heart diseases, hypertension, hyperlipemia and hepatitis. Interestingly, I. khasiana is relatively unknown and yet it is known to possess multiple medicinal characteristics. The antioxidant potential studies were conducted on leaf extract specimens from Mizoram, using petroleum ether (IKP), chloroform (IKC) and methanol (IKM). IKM had the highest total antioxidant activity with 76.42 ascorbic acid equivalent (AE) mg/g, followed by IKP with 44.27 AE mg/g, and then by IKC with 17.08 AE mg/g respectively. The reducing power assay showed a concentration-dependent activity against potassium ferricyanide. The total phenolic content was found to be 3.46 gallic acid equivalent (GAE) mg/g for IKM and 1.450 GAE mg/g for IKC respectively. The total flavonoid content was 41.9 quercetin equivalent (QE) mg/g for IKC and 30.8 QE mg/g for IKM. IKP did not show any activity in phenolic and total flavonoid assays.Keywords
Antioxidant Activity, Flavonoids, Ilex khasiana, Phenolics, Reducing Power.Full Text
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