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Bindhu, J.

Comparative Analysis of Bioactive Compounds and Anticancerous Activities in Leaf and Stem Extract of Physalis minima

Abstract Views :250 | PDF Views:198

Authors

Arunava Das ¹, Aileen Rose Edwin ¹, R. Krushnakeerthana ¹, J. Bindhu ¹

Affiliations
1 Molecular Diagnostics and Biomolecular Characterization Laboratory, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode District, Tamil Nadu, IN

Source

Journal of Natural Remedies, Vol 20, No 1 (2020), Pagination: 42-52

Abstract

Physalis minima, commonly known as native gooseberry is a perennial herb which grows mostly in the tropical regions and is used in the traditional medicines as a curative for gastric ulcers and also for respiratory tract related ailments. The leaf and stem crude extract was separated by soxhelet using chloroform as solvent followed by the phytochemical screening to identify the presence of secondary metabolites. The Fourier-transform infrared spectroscopy (FTIR) showed the presence of various functional group such as phenol, alkane, aldehyde, alkyls, amino acid, aromatic amino acids and secondary alcohol. The Gas chromatography-mass spectrometry (GC-MS) result showed the presence of total of 24 bioactive compounds in both extracts such as Heptacosane, 1-chloro, Heptacosane, Octadecanoic acid, N-hexadecanoic acid and Hexatriacontane. The extract was subjected to for its anti-cancerous activity using MTT assay which had a good result against HL60 cell line and antibacterial activities.

Keywords

HL60, MTT Assay, Physalis minima, Phytochemical Screening, Soxhlet Extraction.

Full Text

References

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Nathiya M, Dorcus D. Preliminary phytochemical and antibacterial studies on Physalis minima Linn. International Journal of Current Science. 2012:24–30.

Parmar C, Kaushal MK. Physalis minima. In: Wild Fruits. New Delhi, India: Kalyani Publishers; 1982.

Tiger L. The natural guide to the medicinal herbs and plants (1st ed.), Tigerbooks, PLS, Twitchenhanze, UK; 1980. p. 12–15.

Karpagasundari C, Kulothungan S. Analysis of bioactive compounds in Physalis minima leaves using GC MS, HPLC, UV-VIS and FTIR techniques. Journal of Pharmacognosy and Phytochemistry. 2014; 3(4):196–201.

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Agbor AG, Talla L, Ngogang JY. The Antidiarrhoeal activity of Alchornea cordifolia leaf extract. Phytotherapy Research: PTR. 2004; 18(11):873–6.

Chothani DL, Vaghasiya HU. A phyto-pharmacological overview on Physalis minima Linn. Indian Journal of Natural Products and Resources. 2012; 3:477–82.

Ramkumar KM, Rajaguru IP, Ananthan ZR. Antimicrobial properties and phytochemical constituents of an antidiabetic plant Gymnema montanum. IDOSI publications. Advances in Biological Research. 2007; 1(1–2):67–71.

Aqil F, Ahmed I, Mehmood Z. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turkish Journal of Biology. 2006; 30:177–83.

Sumathy V, Lachumy SJ, Zakaria Z, Sasidharan S. In vitro bioactivity and phytochemical screening of Musa acuminata flower. Pharmacologyonline. 2011; 2:118–27.

Antibacterial and in vitro Anticancer Study of Methanol Extracts of Clitoria ternatea Leaves

Abstract Views :309 | PDF Views:401

Authors

Arunava Das ¹, G. Shanmuga Priya ¹, S. Soundariya ¹, P. Deepesh ¹, Aileen Rose Edwin ¹, E. Vihashinee ¹, A. Rubiga ¹, S. Megavarthini ¹, R. Eswaran ¹, J. Bindhu ¹

Affiliations
1 Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode, IN

Source

Journal of Natural Remedies, Vol 20, No 2 (2020), Pagination: 96-102

Abstract

Clitoria ternatea is often known as butterfly pea plant by the natives and is one of the major plants used in Ayurveda and traditional medicine worldwide. It is also used in food and cosmetic industries for various purposes. In this study, methanol leaf extract of Clitoria ternatea was studied for its antibacterial and in vitro anticancer study against human promyelocytic leukemia cells (HL60). Secondary metabolites like alkaloids, phlobatannin, triterpenoids, flavonoids, lipids, steroids, terpenoids, tannins and glycosides are identified in the extract by phytochemical screening. Leaf extract showed the presence of ethers and carboxylic acid groups (GCMS analysis). The leaf extract showed resistance against Salmonella typhi. Thus, from this study it can be concluded Clitoria ternatea leaves methanol extract has bioactive compounds showing anticancer activity against HL60 cells.

Keywords

Antibacterial Activity, Anticancer Activity, Clitoria ternatea, GC-MS Analysis, Phytochemical Screening, MTT Assay.

Full Text

References

Balaji KS, Priyanka S, Preethi SD, Chandrashekara KT, Lokesh S. Angio-suppressive effect of Clitoria ternatea flower extracts is mediated by HIF-1α and down-regulation of VEGF in Murine carcinoma model. Medicinal Chemistry (Los Angeles). 2016; 6:515–20. https://doi.org/10.4172/2161-0444.1000392

Chakraborthy GS, Kumar V, Gupta S, Kumar A, Gautam N, Kumari L. Phytochemical and pharmacological aspects of Clitoria ternatea-a review. Journal of Applied Pharmaceutical Sciences and Research. 2018:3–9. https://doi.org/10.31069/apsr.v1i2.13061

Deepika S, Selvaraj I, Anbalagan M. Phytochemical characterization and cancer cell line cytotoxicity of Clitoria ternatea. Bangladesh Journal of Pharmacology. 2018; 13(4):349–52. https://doi.org/10.3329/bjp.v13i4.38612

Divya A, Anbumalarmathi J, Sharmili SA. Phytochemical analysis, antimicrobial and antioxidant activity of Clitoria ternatea blue and white flowered leaves. Advances in Research. 2018:1–13. https://doi.org/10.9734/AIR/2018/39030

Lakshmi NDM, Mahitha B, Madhavi T, Sushma NJ. Phytochemical screening and ftir analysis of Clitoria ternatea leaves. International Journal of Scientific and Engineering Research. 2015; 6(2):287–90.

Jacob L, Latha MS. Anticancer activity of Clitoria ternatea Linn. against Dalton’s lymphoma. International Journal of Pharmacognosy and Phytochemical Research. 2012; 4(4):207–12.

Jamil N, Zairi MNM, Nasim NAIM, Pa’ee F. Influences of environmental conditions to phytoconstituents in Clitoria ternatea (Butterfly Pea Flower)-A Review. Journal of Science and Technology. 2018; 10(2).

Kamilla L, Mansor SM, Ramanathan S, Sasidharan S. Effects of Clitoria ternatea leaf extract on growth and morphogenesis of Aspergillus niger. Microscopy and Microanalysis. 2009; 15(4):366–72. https://doi.org/10.1017/S1431927609090783. PMid:19575837

Kumar D, Dhobi M. Antianxiety and antioxidant profile of blue and white variety of Clitoria ternatea L. Indian Journal of Research in Pharmacy and Biotechnology. 2016; 4(3):90.

Lijon MB, Meghla NS, Jahedi E, Rahman MA, Hossain I. Phytochemistry and pharmacological activities of Clitoria ternatea. International Journal of Natural and Social Sciences. 2017; 4(1):1–10.

Mathew N, Anitha MG, Bala TSL, Sivakumar SM, Narmadha R, Kalyanasundaram M. Larvicidal activity of Saracaindica, Nyctanthesarbor-tristis, and Clitoria ternatea extracts against three mosquito vector species. Parasitology Research. 2009; 104(5):1017–25. https://doi.org/10.1007/s00436-008-1284-x. PMid:19039604

Nguyen KNT, Nguyen GKT, Nguyen PQT, Ang KH, Dedon PC, Tam JP. Immunostimulating and Gram‐negative‐specific antibacterial cyclotides from the butterfly pea (Clitoria ternatea). The FEBS Journal. 2016; 283(11):2067–90. https://doi.org/10.1111/febs.13720. PMid:27007913

Pasukamonset P, Kwon O, Adisakwattana S. Alginatebased encapsulation of polyphenols from Clitoria ternatea petal flower extract enhances stability and biological activity under simulated gastrointestinal conditions. Food Hydrocolloids. 2016; 61:772–9. https://doi.org/10.1016/j.foodhyd.2016.06.039

Kumar TR, Kumar S, Anju VS. Phytochemical and antibacterial activities of crude leaf and ischolar_main extracts of Clitoria ternatea varieties (Fabaceae). Journal of Pharmacognosy and Phytochemistry. 2017; 6(6):1104–8.

Sarumathy K, Rajan MD, Vijay T, Jayakanthi J. Evaluation of phytoconstituents, nephro-protective and antioxidant activities of Clitoria ternatea. Journal of Applied Pharmaceutical Science. 2011; 1(5):164–72.

Antimicrobial, Antioxidant and Anticancer Activity of the Ethanol Extract of Pleurotus ostreatus

Abstract Views :280 | PDF Views:241

Authors

J. Bindhu ¹, Arunava Das ¹, E. Vihashinee ¹, A. Rubiga ¹, Aswin Edward ¹, R. Eswaran ¹, G. Shanmuga Priya ¹, S. Soundariya ¹, Aileen Rose Edwin ¹

Affiliations
1 Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode District, Tamil Nadu, IN

Source

Journal of Natural Remedies, Vol 20, No 2 (2020), Pagination: 110-115

Abstract

The current study evaluates antimicrobial, antioxidant and anticancer activity of the ethanol extract of Pleurotus ostreatus. Chemical analysis showed the presence of alcohol, amides, alkanes, ether, phenol and sulphides. Most of the chemical constituents were found to be esters and carboxylic acid derivatives. The extract showed highest resistant against Bacillus cereus. P. ostreatus ethanol extract showed comparable antioxidant activity with ascorbic acid in both DPPH and FRAP method and also showed an IC₅₀ of 39.29 μg/ml against HL60 cells.

Keywords

MTT, DPPH, HL-60 Cells, FRAP, GC-MS.

Full Text

References

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Jose N, Ajith TA, Janardhanan KK. Antioxidant, anti-inflammatory and antitumor activities of culinary-medicinal mushroom Pleurotus pufmonanus (Fr.) Quel. (Agaricomycetideae). International Journal of Medicinal Mushrooms. 2002; 4:329–35. https://doi.org/10.1615/IntJMedMushr.v4.i4.60

Jayakumar T, Ramesh E, Geraldine P. Antioxidant activity of the oyster mushroom Pleurotus ostreatus on CCl4-induced liver injury in rats. Food and chemical toxicology. 2016; 44:1989–96. https://doi.org/10.1016/j.fct.2006.06.025.PMid:16914248

Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer, Chemical Biological Interaction. 2006; 160:1–40. https://doi.org/10.1016/j.cbi.2005.12.009. PMid:16430879

Woldegiorgis AZ, Abate D, Haki GD, Ziegler GR. Antioxidant property of edible mushrooms collected from Ethiopia. Food Chemistry. 2014; 157:30–6. https://doi.org/10.1016/j.foodchem.2014.02.014. PMid:24679748

Michalak A. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish Journal of Environmental Studies. 2006; 15:523–30.

Oyetayo VO, Ariyo OO. Antimicrobial and antioxidant properties of Pleurotus ostreatus (Jacq: Fries) cultivated on different tropical woody substrates. Journal of Waste Conversion, Bioproducts and Biotechnology. 2013; 2:28–32.

Oboh G, Ademosun AO. Characterization of the antioxidant properties of phenolic extracts from some citrus peels. Journal of Food Science and Technology. 2012; 49:729–36. https://doi.org/10.1007/s13197-010-0222-y. PMid:24293692 PMCid:PMC3550823

Vishwakarma P, Singh P, Tripathi NN. In-vitro antioxidant activity and nutritional value of four wild oyster mushroom collected from North-Eastern Part of Uttar Pradesh. Mycosphere. 2017; 8:592–602. https://doi.org/10.5943/mycosphere/8/4/8

Singh V, Pandey R, Vyas D. Antioxidant potentiality of Pleurotus ostreatus (MTCC142) cultivated on different agro wastes. Asian Journal of Plant Science and Research. 2015; 5:22-7.

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Authenticating the Anti-cancer Properties of Couroupita guianensis in Western Ghats using HL60 Humanleukemia Cell Line

Abstract Views :247 | PDF Views:122

Authors

J. Bindhu ¹, R. Felicia Roshini ¹, M. Monica Devi ¹, Arunava Das ¹, R. Balakrishna Raja ¹, S. Tamilselvi ¹

Affiliations
1 Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Tamil Nadu, IN

Source

Journal of Natural Remedies, Vol 21, No 2 (2021), Pagination: 125-132

Abstract

The target of this work is to evaluate the in–vitro anticancer activity and cellular toxicity of Couroupita guianensis (CG) towards the Human promyelocytic leukemia HL60 cells contrasted with the normal cell lines. The anti-cancer properties of Couroupita guianensis extracted with Chloroform and DMSO (Dimethyl sulfoxide) were analysed and studied throughout this paper. HL60 cell lines were permitted to develop in DMEM (Dulbecco's Modified Eagle's medium) and incubated with differed concentration of DMSO CG extract. MTT assay drew out the best approach to decide the cell feasibility and assessment was done with the optical absorbance at 570nm and 620nm as reference. The floral extract inhibited 50% growth (IC₅₀) of HL60 cell lines at 10 mg/ml of extract concentration. Inhibitory adequacy of CG showed the cell – plausibility in time and dosage relied route with predictable morphological changes. Flow cytometer assessed the apoptosis insisting the cell toxicity esteem for MTT at IC₅₀ with 70 % cell suitability. Morphological investigation likewise plainly expresses that no apoptosis has been found in charge and comparably in CG treated when contrasted with destructive HL60 cell – line. Assessment of cell toxicity impact of CG demonstrated it can be an inert source of great transformation in human Acute promyelocytic leukemia indicating (revealing) that chemotherapeutic agent.

Keywords

Cellular Toxicity, Growth Inhibition, Leukemia DMSO

Full Text

Evaluation of Antioxidant and Antimicrobial Potential of Green Synthesized Ag Nanoparticles from Ethanolic Leaf Extract of Thespesia populnea

Abstract Views :174 | PDF Views:83

Authors

S. Charulatha ¹, H. V. Dharrunya ¹, R. Dhanamurugan ¹, R. Devadharshini ¹, Debanjoy Paul ¹, M. E. Sinduja ¹, J. Bindhu ²

Affiliations
1 Department of Biotechnology, Bannari Amman Institute of Technology, Sathymangalam, Erode District, Tamil Nadu, IN
2 Department of Biotechnology, Sri Shakthi Institute of Engineering & Technology, Coimbatore, Tamil Nadu, IN

Source

Journal of Natural Remedies, Vol 22, No 2 (2022), Pagination: 137 - 144

Abstract

Thespesia populnea is a variety of small tree found mostly in the tropical regions of the world belonging to the family of Malvaceae. According to ancient and traditional medicine such as Ayurveda this plant is used to treat various diseases such as bloody diarrhea, fracture healing, urinary tract problem, abdomen swelling, pleurisy, skin disorders, high fever and menstrual issues. This study focuses on the evaluation of antioxidant and antimicrobial activities of synthesized silver nanoparticles from aqueous leaf extract of Thespesia populnea plant. Reducing capability of synthesized nanoparticles was examined by carrying out various antioxidant assays such as reduction of DPPH substrate through radical scavenging. It showed the maximum reduction of DPPH as 92.77% at 120 &#956g/mL concentration. At 120 &#956g/mL concentration, phosphomolybdenum showed highest contraction percentage ranging 51.47 % and followed by Fe3+ reduction at 28.78 %. Studies on antimicrobial activity has revealed that the maximum zone of inhibition was exhibited by Proteus vulgaris with distinct zone formation up to 19 mm diameter.

Keywords

Antioxidant, Antimicrobial, DPPH· Radical, Phosphomolybdenum

Full Text

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H, Al-Qudah M, et al. Antimicrobial activity of crude extracts of some plant leaves. Res. J. Microbiol. 2012;7(1):59–67.

https://doi.org/10.3923/jm.2012.59.67 .

Jayasri A, Prasad PE, Padmaja K, Kumar BDPK, Gnanaprakash M, Kavitha K. Antibacterial activity of Thespesia populnea mediated nanoparticles. Int J Curr Microbiol App Sci. 2021; 10(02):913–18. https://doi.org/10.20546/ijcmas.2021.1002.107 .

Elshawy OE, Helmy EA, Rashed LA. Preparation, characterization, and in vitro evaluation of the antitumor activity of the biologically synthesized silver nanoparticles. Advances in Nanoparticles. 2016; 5:149–66. https://dx.doi.org/10.4236/anp.2016.52017 .

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Jayasri A, Prasad PE, Padmaja K, Kumar BDPK, Gnanaprakash M, Sonali M. In vitro and in vivo safety evaluation of Thespesia populnea mediated nanoparticles. Pharma Innovation. 2021; 10(1):09–11.

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