Journal of Natural Remedies

V. P. Veerapur , M. B. Palkar , H. Srinivasa , M. S. Kumar , S. Patra , P. G. M. Rao , K. K. Srinivasan.

Abstract

Objectives: To screen the wound healing activity of ethanol extract of Wrightia tinctoria bark using incision, excision and dead space wound models and evaluate histopathological and biochemical changes of granuloma tissue. Material and method: The bark powder of W. tinctoria was extracted with 95% ethanol by continuous heat extraction and was subjected for phytochemical investigation and screened for wound healing activity in the incision, excision and dead space wound models in rats. A supportive study made on granuloma tissue to estimate the hydroxyproline content and histopathological examination to determine the pattern of lay-down for collagen using Masson Trichrome stain. Results: Triterpenoids, steroids and saponins were found to be present in ethanol extract of the barks of W. tinctoria. In resutured incision wound model, ethanol extract showed significant breaking strength (P<0.01) compared to control. The ethanol extract promotes better wound healing by increasing the percentage wound closure and decreasing epithelization time (P<0.001) compared to control. Statistically significant increase (P<0.001) was observed in breaking strength and hydroxyproline content of ten day old granuloma of drug treated animals compared to control animals in dead space wound model. Conclusion: The results of the present study reveal that ethanol extract of bark of W. tinctoria have significant wound healing activity. The pro-healing action seems to be due to the increased synthesis of collagen, it's cross-linking as well as better alignment and maturation. This may be attributed to the presence of triterpinoids in the title plant.

Keywords

Wrightia tinctoria, Wound Healing Activity, Hydroxyproline, Histopathology

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Sheetal Anandjiwala , Milind S. Bagul , H. Srinivasa , Jyoti Kalola , M. Rajani

Abstract

Objective: To evaluate the antioxidant activity of stem bark of Thespesia populnea. Materials and Method: Antioxidant activity of T. populnea stem bark was studied in four in vitro / ex vivo models, viz., DPPH radical scavenging activity, superoxide scavenging activity, reducing power assay and lipid peroxidation. Results: Preliminary phytochemical screening showed the presence of high amount of phenolics, tannins and flavonoids. Subsequent quantification by Folin Ciocalteau method showed the presence of high amount of total phenolics (10.11 % w/w, calculated as gallic acid). Methanolic extract of stem bark of T. populnea showed a very good DPPH radical scavenging activity (EC₅₀ of 12.08 μg) and superoxide scavenging activity (EC₅₀ of 73.53 μg) in a dose dependent manner. The reduction ability of the extract, (Fe³⁺ to Fe2+ transformation) was found to increase with increasing concentrations where a maximum absorbance of 1.012 was obtained at a concentration of 300 μg. It also inhibited lipid peroxidation in a dose dependent manner with an EC₅₀ of 20.76 μg, which was comparable to α-tocopherol (EC₅₀ of 27.35 μg). Conclusion: The methanolic extract of the stem bark of T. populnea showed very good free radical scavenging activity, including inhibition of lipid peroxidation and reducing power.

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