Journal of Natural Remedies

T. Prakash , Dayanand Bhoumik , H. Shivakumar , J. S. Venkatesh , A. S. Dutta

Abstract

Objective: To evaluate the different extracts of Psidium guajava bark for its antidiarrhoeal potential against several experimental models of diarrhoea in rats. Materials and methods: Antidiarrhoeal activity of methanolic and aqueous extracts of Psidium guajava bark was studied at dose of 50 mg/kg and 100 mg/kg body weight against gastrointestinal motility, castor oil-induced diarrhoea and prostaglandin E₂-inducead enteropooling (PGE₂) in rats. Results: The methanolic and aqueous extracts of P. guajava bark showed significant antidiarrhoeal activity in a dose dependent manner against all the experimental diarrhoea models. The potent antidiarrhoeal activity may be attributed to the presence of tannin and quercetin. Conclusion: The results indicated that both the extracts of P. guajava bark at dose of 100 mg/kg were found to be more potent than the reference standard drugs, loperamid and atropine.

Keywords

, Bark

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H. Shivakumar , Talha Javed , T. Prakash , R. Nagendra Rao , B. H. M. Jayakumar Swamy , A. Veerana Goud

Abstract

Objective: To investigate the adaptogenic activity (anti-stress) of the ethanolic extract of Tribulus terrestris at various doses using experimentally induced stress models in Mice and Rats. Methods: Anoxia stress tolerance, Swimming endurance, Immobilization and Cold stress models were used for evaluation of adaptogenic activity. Withania sominifera (100mg/kg, po) was used as reference standard and it showed significant adaptogenic activity in all four models of stress. The parameters like Anoxia stress tolerance time and swimming endurance time were measured for anoxia induced stress tolerance and swimming endurance models respectively. However for other two models organ weight and biochemical marker levels were estimated in negative control, positive control and drug treated groups. Result: Concomitant treatment with ethanolic extract at 100, 300 and 500 mg/kg showed marked increase in anoxia stress tolerance time and swimming endurance time as compared to control group in anoxia stress tolerance and swimming endurance tests. Similarly, concomitant treatment with ethanolic extract at different doses showed marked decrease in blood glucose, cholesterol, triglycerides and BUN level as compared to stress control in both immobilization stress and cold stress models. Weight of the liver and adrenal gland are markedly decreased but no weight changes were observed in spleen and testes in both the stress models. Conclusion: The present study suggests that ethanolic extract of T. terrestris L. possess a significant adaptogenic property and its incorporation in the Siotone an Ayurvedic rasayana is justifiable.

Keywords

Tribulus terrestris, Adaptogenic, Biochemical Estimation

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H. Shivakumar , T. Prakash , R. Nagendra Rao , B. H. M. Jayakumar Swamy , A. N. Nagappa

Abstract

Objective: To screen the wound healing activity of petroleum ether, alcohol and aqueous extracts of leaves of Thespesia populnea on excision and incision wound models in albino rats. Materials and methods: The different extracts of the leaves of Thespesia populnea are obtained by successive soxhlet extraction with petroleum ether, alcohol and aqueous were subjected to acute toxicity studies. The extracts were screened for wound healing properties in the excision and incision wound models in albino rats of either sex under light ether anesthesia. Result: All the three extracts showed significant increase in wound contraction and formation of scar in excision wound model. These extracts showed significant increase in the breaking strength of resutured incision wound as compared to control group (P<0.001). Glycosides, flavonoids, alkaloids, and phytosterols have been reported to be present in leaves of T. populnea. Conclusion: The results of the present study indicate that aqueous extract of T. populnea leaves has more significant wound healing property than the petroleum ether and alcoholic extracts in excision and incision wound models. The presence of flavonoids, alkaloids and phytosterols in the leaves of the plant may be responsible for wound healing activity.

Keywords

Thespesia populnea, Incision and Excision Wound Models

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