Journal of Natural Remedies

B. Murali , A. Amit , M. S. Anand , D. S. Samiulla

Abstract

Objective: Estimation of wedelolactone (WDL) and demethylwedelolactone (DWDL) in Eclipta alba Hassk. by HPLC. Materials and methods: An Isocratic, reverse phase (RP) HPLC procedure for the standardization of Eclipta alba using a mixture of 0.1% v/v Ortho phosphoric acid and acetonitirile as mobile phase C8 column as stationary phase and photo diode array detector. Results: The above method shows high resolution, accuracy, economical and reproducibility. Conclusions: The method developed is useful in standardization of Eclipta alba material.

Keywords

Eclipta alba, Wedelolactone, Demethylwedelolactone, HPLC

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D. S. Samiulla , M. S. Harish

Abstract

Objective: To study the comparative effect of NR-AG-I and NR-AG-II (polyherbal formulations) against gentamicin- induced nephrotoxicity in rats. Materials and methods: Nephroprotective activity was evaluated in gentamicin induced nephrotoxicity (80mg/kg/day s.c) in male albino rats. NR-AG-I and NR-AG-II were tested at a dose of 150mg/kg/day p.o. All the treatments were given for 12days. Urea clearance and microscopical examinations of kidney performed after the treatment. Results: Gentamicin treatment caused nephrotoxicity as evidenced by significant decrease in urea clearance and was prevented by both formulations. Study of renal microscopy showed necrosis, epithelial loss with granular degeneration and fatty changes in gentamicin treated rats and was reversed by both formulations, but NR-AG-I proven to be better formulation in our experimental study. Conclusion: Our data suggest that treatment with NR-AG-1 and NR-AG-II may be useful in reducing gentamicin nephrotoxicity in rats.

Keywords

NR-AG-I, NR-AG-II, Nephrotoxicity, Gentamicin, Urea Clearance

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B. Murali , A. Amit , M. S. Anand , T. K. Dinesh , D. S. Samiulla

Abstract

Objective: To develop an improved HPLC method for estimation of phyllanthin and hypophyllanthin in Phyllanthus amarus. Materials and methods: An Isocratic, reversed phase (RP) HPLC procedure has been adopted using a mixture of pH 2.8 Phosphate buffer and acetonitrile as mobile phase, CN column as stationary phase and UV detector. Results: The developed method shows high resolution (R = 1.9), accuracy and reproducibility. Conclusion: The method developed is relatively better in terms of separation than the previously reported methods.

Keywords

Phyllanthus Amarus, Phyllanthin, Hypophyllanthin, HPLC

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D. S. Samiulla , D. Prashanth , A. Amit , B. V. Venkataraman

Abstract

Four successive extracts (petroleum ether, chloroform, methanol and water) of P. granatum fruit rind were tested for in-vitro trypsin inhibitory activity using commercially available enzyme (Porcine pancreatic type II trypsin) and substrate (Benzoyl-D,L-arginine-4-nitroanilide) and the activity was compared with standard trypsin inhibitor from hen egg white (ovomucoid) . The methanolic extract was found to be most active and the activity was comparable to that of the reference standard.

Keywords

Punica Granatum, Ovomucoid, Trypsin Inhibitor, Proteases

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