Journal of Natural Remedies

Nayana S. Kapadia , Niyati S. Acharya , B. N. Suhagia , Mamta B. Shah

Abstract

Objective: Development of physico-chemical parameters and estimation of scopoletin, a chemical marker in herb of Convolvulus prostratus Forssk. (Family: Convolvulaceae). Materials and methods: Fresh flowering plants of Convolvulus prostratus, commonly known as Shankhpushpi, were studied for developing quality parameters and HPTLC method for quantification of scopoletin and fingerprinting of C. prostratus using a mobile phase toluene and ether (1:1, saturated with 10% glacial acetic acid) and instrument Camag Linomat IV with Camag TLC scanner. Results: Morphologically, the herb can be distinguished on the basis of hairy nature, sessile leaves, white-pale pink flowers and woody ischolar_mainstalk. Leaves show striated cuticle, rubiaceous and cruciferous stomata, calcium oxalate prisms, and bicollateral vascular bundle. Stem shows unicellular covering trichomes, a discontinuous ring of pericyclic fibres and pitted cells in pith. Root is easily characterized by intra-xylary phloem. Spherical pollen grains and large number of unicellular trichomes are seen in powder of C. prostratus. HPTLC method was developed for determination of scopoletin content by scanning the plates at 366 nm. Conclusions: The histological characters and HPTLC method developed for quantification of scopoletin and fingerprinting of C. prostratus would serve as a useful tool in standardization of C. prostratus.

Keywords

Convolvulus Prostratus, Scopoletin, HPTLC

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Patel Mayuree ¹, Macwan Carol ², B. N. Suhagia ³, Sanjeev R. Acharya ⁴

Affiliations
1 Institute of Pharmacy, Nirma University, S. G. Highway, Ahmedabad - 382481, Gujarat, IN
2 Department of Pharmacognosy, Dharmsinh Desai University, College Road, Nadiad - 387001, Gujarat, IN
3 Department of Pharmaceutical Chemistry, Dharmsinh Desai University, College Road, Nadiad - 387001, Gujarat, IN
4 S. S. R. College of Pharmacy (Affiliated to Savitribai Phule Pune University, Pune), Sayli, Silvassa - 396230, Maharashtra, IN

Abstract

Objective: Guggul is the oleo-gum-resin obtained from deep incisions at the basal part of stem bark of Commiphora wightii belonging to Burseraceae family. It is very popular ancient Ayurvedic medicine used to cure various diseases. In Ayurveda guggul is always purified prior to use in different formulations. This process is known as sodhana. Guggulsterone E and Z are the prime constituents of Commiphora wightii. The main objective of this study was to identify the impact of guggul sodhana process mentioned in Ayurvedic Formulary of India (AFI) on the content of guggulsterone E and Z. The methanol extract of raw guggul and seven different sodhit guggul were qualitatively and quantitatively analysed by using LC-MS and HPLC methods. The LC-MS specrum indicated the presence of guggulsterone E&Z at m/z-313.2 in all samples with a retention time 4.8 min and 5.7 min respectively. The HPLC chromatogram at 245 nm showed guggulsterone E & Z with same retention time in all samples. The study reports that all the sodhit guggul samples contain guggulsterone E&Z. The water shodhit guggul showed 95.95% of total guggulsterone while triphala shodhit guggul showed 74.21%. It is suggested that sodhana process affects the quantity of guggulsterone E&Z but do not modify its chemical properties.

Keywords

Commiphora wightii, Shodhan Dravya, Sodhana Process.

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