Journal of Natural Remedies

Eni Widayati ¹, Chodidjah ², Taufiqurrachman Nasihun ³

Affiliations
1 Department of Chemistry, Sultan Agung Islamic University, Jawa Tengah - 50112, ID
2 Department of Anatomy, Sultan Agung Islamic University, Jawa Tengah - 50112, ID
3 Department of Biochemistry, Sultan Agung Islamic University, Semarang, Jawa Tengah - 50112, ID

Abstract

Introduction: Incidence of breast cancer is high and constitutes a significant cause of female mortality. One of breast cancer type is Estrogen and Progesteron Receptor (ER and PR) positive, therefore affected by estrogen and progesteron concentration. Treatment of Thyponium flageliforme in combination with Curcuma zedoaria, and Phyllanthus niruri (TCP) have been proven capable of inhibiting tumor cells proliferation. Objective: To evaluate the expression of protein estrogen receptor and caspase-9 in C3H mice were inoculated with adenocarcinoma cells. Methods: 18 mice were inoculated with one million cells of breast adenocarcinoma per mouse and assigned into three groups. TCP with dosage 85 and 125 mg were treated for 21 days to evaluate the expression of protein estrogen receptor and caspase-9 measured by immuno-histochemical staining. Results: Mann Whitney analysis demonstrated that expression of protein estrogen receptor and caspase-9 in TCP 125 was significant (p < 0.05). Conclusion: Expression of protein estrogen receptor was decreased and caspase-9 expression was increased by TCP administration.

Keywords

Breast Cancer, Caspase9, Combination of Herbal Extracts, Estrogen Receptors.

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Titiek Sumarawati ¹, Taufiqurrachman Nasihun ², Chodidjah ³

Affiliations
1 Department of Chemistry, Sultan Agung Islamic University, Central Java Tengah – 50112, ID
2 Department of Biochemistry, Sultan Agung Islamic University, Semarang, Central Java – 50112, ID
3 Department of Anatomy, Sultan Agung Islamic University, Central Java – 50112, ID

Abstract

Introduction: People worldwide drink Zamzam water either medicinally or religiously. However, the effect of zamzam water extract of CZ in breast cancer cells remains unclear. Objective: To evaluate the zamzam water extract of CZ effect on cell apoptosis, p53 and Bcl2 protein expression in T47 D cells line culture. Methods: In the control group design, 16 Wells which was filled with T47 D cell line culture in the density of 5 × 10⁴/100 μl, were assigned into 4 groups. Control group (C-G), were only filled with T47D cell line culture; Zamzam 50 (ZZ-50); Zamzam 100 (ZZ-100), and Zamzam 500 (ZZ-500) groups, were filled with T47D cell line cultures and 50, 100, and 500 μg/ml zamzam water extract of CZ respectively, and then incubated for 48 hours. The number of apoptotic cell was counted by flow cytometry, while p53 and Bcl2 measured by immunohistochemistry staining method. Results: Mann Whitney analysis indicated that the percentage of apoptotic cell and p53 protein expression in ZZ-100 was significant higher compared to that of C-G (p < 0.05). In contrary the percentage number of Bcl2 protein expression in ZZ100-G was significantly lower when compared to that of C-G (p < 0.05). Conclusion: Treatment of zamzam water extract of CZ on T47D cell line cultured was capable of increasing the number of apoptotic cells, p53 protein expression, but decreased Bcl2 protein expression in T47D cell line culture. However the numbers of apoptotic cells were less than 10%.

Keywords

Apoptosis, Breast Cancer, Chemoprevention, Flavonoids, P53 Mutan.

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