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Tamarindus indica (Cesalpiniaceae), and Syzygium cumini (Myrtaceae) Seed Extracts can Kill Multidrug Resistant Streptococcus mutans in Biofilm


Affiliations
1 Institute of Science, Nirma University, S-G Highway, Ahmedabad, 382481, India
 

Extracts of Emblica officinalis seeds prepared by Microwave Assisted Extraction (MAE) method were evaluated for their antimicrobial property against planktonic form of certain human/plant pathogenic microbes. Additionally, seed extracts of E. officinalis, Tamarindus indica, Manilkara zapota, Phoenix sylvestris, Syzygium cumini, and selected phytocompounds were tested against multi-drug resistant Streptococcus mutans (a major pathogen associated with human dental caries) in its planktonic as well as biofilm form. Ability of these extracts to eradicate and kill S. mutans biofilm was investigated. E. officinalis extracts exerted bactericidal action against S. mutans, Pseudomonas aeruginosa, and Vibrio cholerae. Acetone extract of S. cumini, and curcumin were able to inhibit S. mutans at appreciably low concentrations of 50 μg/mL and 20 μg/mL respectively. T. indica and S. cumini seed extracts were able to kill ≥ 80% cells of S. mutans in biofilm, in the concentration range of 500-1000 μg/mL. These extracts were able to achieve ≥ 95% killing of S. mutans biofilm at concentrations ranging from 600-2000 μg/mL. Ability of the potent extracts to kill S. mutans biofilm did not seem to be much dependent on eradication of the biofilm. Extraction efficiency was found to have a good correlation with antibacterial activity.

Keywords

Antibacterial, Biofilm, Drug-resistance, Microwave Assisted Extraction (MAE), Minimum Inhibitory Concentration (MIC), Emblica officinalis
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  • Talaro KP. Foundation in Microbiology: Basic Principles. New York: McGraw-Hill; 2008.

  • Buchanan BB, Gruissen W, Jones RL, editors. Biochemistry and molecular biology of plants. India: I. K. International Pvt. Ltd.; 2000.

  • Yigit D, Yigit N, Mavi A. Antioxidant and antimicrobial activity of bitter and sweet apricot (Prumus armeniaca L.) kernels. Braz J Med Res. 2009 Apr; 42(4):346–52.

  • Mah TFC, O’Toole GA. Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol. 2001 Jan 1; 9(1):34–9.

  • Chen L, Wen Y. The role of bacterial biofilm in persistent infections and control strategies. Int J Oral Sci. 2011; 3:66–73. DOI. 10.4248/IJOS11022

  • Hasan S, Danishuddin M, Adil M, Singh K, Verma PK, Khan AU. Efficacy of E. officinalis on the cariogenic properties of Streptococcus mutans: a novel and alternative approach to suppress quorum-sensing mechanism. PLoS ONE. 2012 Jul 5; 7(7):1–12.

  • Kunze B, Reck M, Dötsch A, Lemme A, Schummer D, Irschik H, Wagner-Döbler I. Damage of Streptococcus mutans biofilms by carolacton, a secondary metabolite from the myxobacterium Sorangium cellulosum. BMC microbiol. 2010 Jul 26; 10(1):199–211.

  • Rukayadi Y, Hwang JK. In vitro activity of xanthorrhizol against Streptococcus mutans biofilms. Lett Appl Microbiol. 2006 Apr; 42(4):400–4.

  • Hosseini F, Adlgostar A, Sharifnia F. Antibacterial Activity of Pistacia atlantica extracts on Streptococcus mutans biofilm. Int Res J Biological Sci. 2013 Feb; 2(2):1–7.

  • Kothari V, Punjabi A, Gupta S. Optimization of microwave assisted extraction of Annona squamosa Seeds. The Icfai Univ J Life Sci. 2009; 3(1):55–60.

  • Kothari V, Seshadri S. In vitro antibacterial activity in seed extracts of Manilkara zapota, Anona squamosa, and Tamarindus indica. Biol Res. 2010 Sep 24; 43(2):165–8.

  • Kothari V. In vitro antibacterial activity in seed extracts of Pheonix sylvestris Roxb (Palmae) and Tricosanthes dioica L (Cucurbitaceae). Curr Trends Biotechnol Pharm. 2011; 5(1): 993–7.

  • Darji B, Ratani J, Doshi M, Kothari V. In vitro antimicrobial activity in certain plant products /seed extracts against selected phytopathogens. Res Pharm. 2012; 2(6):1–10.

  • Ramanuj K, Bachani P, Kothari V. In vitro antimicrobial activity of certain plant products/seed extracts against multidrug resistant Propionibacterium acnes, Malassezia furfur, and aflatoxin producing Aspergillus flavus. Res Pharm. 2012; 2(3):22–31.

  • Jorgensen JH, Turnidge JD. Susceptibility test methods: dilution and disk diffusion methods. In: Murry PR, Washington editors. Manual of clinical microbiology. 7th Ed. New York: ASM International; 2003.

  • Ingroff A, Pfaller MA. Susceptibility test methods: yeasts and filamentous fungi. In: Murry PR, editors. Manual of clinical microbiology. 7th Ed. New York: ASM Press; 2003.

  • Wadhwani T, Desai K, Patel D, Lawani D, Bahaley P, Joshi P, Kothari V. Effect of various solvents on bacterial growth in context of determining MIC of various antimicrobials. Internet J Microbiol. 2009; 7(1). DOI 10.5580/b43

  • Pfaller MA, Sheehan DJ, Rex JH. Determination of fungicidal activities against yeasts and molds: lessons learned from bactericidal testing and the need for standardization. Clin Microbiol Rev. 2004; 17(2): 268–80.

  • Eloff JN. Quantifying the bioactivity of plant extracts during screening and bioassay guided fractionation. Phytomed. 2004; 11(4): 370–1.

  • Borgio JF, Thorat PK, Lonkar AD. Antimycotic and antibacterial activities of Gynandropsis pentaphylla DC extracts and its phytochemical studies. The Int J Microbiol. 2008; 5(2):1–14.

  • Mathur T, Singhal S, Khan S, Upadhyay DJ, Fatma T, Rattan A. Detection of biofilm formation among the clinical isolates of staphylococci: an evaluation of three different screening methods. Indian J Med Microbi. 2006 Jan; 24(1):25–9.

  • Goldman E, Green LH, editors. Practical handbook of microbiology. 2nd ed. Boca Raton: CRC Press; 2009.

  • Ramage G, Walle KV, Wickes B, Piz-ribot JL. Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms. Antimicrob Agents Chemother. 2001 Sep; 45(9):2475–9.

  • Mandal V, Mohan Y, Hemalatha S. Microwave assisted extraction - an innovative and promising extraction tool for medicinal plant research. Phcog Rev. 2007 Jan-May; 1(1):7–18.

  • Kothari V. Screening of various plant products/plant extracts for antimicrobial and antioxidant properties, and to investigate correlation of the latter with phenolic content of the sample. Nirma University: Ph.D thesis; 2011.

  • Choi MJ, Lee E, Lee S, Reza MA, Le S, Gebru E, Rhee M, Park S. The in vitro antibacterial activity of florfenicol in combination with amoxicillin or cefuroxime against pathogenic bacteria of animal origin. Pak Vet J. 2010; 31(2):141–4.

  • Brown NP, Pillar CM, Draghi DC, Grover P, Alluru V, Torres MK, Sahm DF, Sandvang D, Kristensen H-H. Minimum bactericidal concentration (MBC) analysis and time kill kinetic (TK) analysis of NZ2114 against Staphylococci and Streptococci. Poster presented at the 48th Annual Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC) and the Infectious Diseases Society of America (IDSA) 46th Annual Meeting, Washington, DC. 2008. (http://www. eurofins.com/media/694478/ICAAC2008-nz2114%20 timekill%20F1 3963%20v5%20to%20print.pdf).

  • Kognou A, Ngane R, Kuiate J, Mogtomo M, Tiabou A, Mouokeu R, Biyiti L, Zollo P. Antibacterial and antioxidant properties of the methanolic extract of stem bark of Pteleopsis hylodendron (Combretaceae). Chemother Res and Pract. 2011 Mar 3;2011:1–7. DOI. 10.1155/2011/218750

  • Konate K, Kiendrebeogo M., Ouattara M, Souza A, Lamien-Meda M, Nongasida Y, Barro N, Millogo- Rasolodimby J, Nacoulma O. Antibacterial potential of aqueous acetone extracts from five medicinal plants used traditionally to treat infectious diseases in Burkina Faso. Curr Res J Biol Sci. 2011; 3(5): 435–42.

  • Li XZ, Nikaido H, Poole K. Role of mexA-mexB-oprM in antibiotic efflux in Pseudomonas aeruginosa. Antimicrob Agents and Chemother. 1995; 39(9): 1948–53.

  • Gibbons S. Phytochemicals for Bacterial Resistance - strengths, weaknesses and opportunities. Planta Med. 2008; 74(6):594–602.

  • Aneja KR, Joshi R, Sharma C. In vitro antimicrobial activity of Sapindus mukorossi and Emblica officinalis against dental caries pathogens. Ethnobot Leaflets. 2010; 14(4):402–12.

  • Gupta P, Nain P, Sidana J. Antimicrobial and antioxidant activity on Emblica officinalis seed extract. IJRAP. 2012 Jul-Aug; 3(4):591–6.

  • Kothari V, Gupta A, Naraniwal M. Comparative study of various methods for extraction of antioxidant and antibacterial compounds from plant seeds. J Nat Remedies. 2012; 12(2):162–73.

  • Kothari V, Naraniwal M, Gupta A. Effect of certain phytochemicals on Aeromonas hydrophila. Res Biotechnol. 2011; 2(4):20–5.

  • Rais D, Singh JK, Roy N, Panda D. Curcumin inhibits FtsZ assembly: an attractive mechanism for its antibacterial activity. Biochem J. 2008 Feb 15; 410(1):147–155.

  • Kumar S, Narain U, Trapathi S, Misra K. Syntheses of curcumin bioconjugates and study of their antibacterial activities against-lactamase producing microorganisms. Bioconjug chem. 2001 Jul-Aug; 12(4): 464–9.

  • Martins CVB, Silva DL, Neres ATM, Magalhaes TFF, Watanabe GA, Modolo LV, Sabino AA, Fatima A, Resende M.A. Curcumin as a promising antifungal of clinical interest. J. Antimicrob. Chemoth. 2009 Nov 26; 63(2):337–9.

  • Cui L, Miao J, Cui L. Cytotoxic effect of curcumin on malaria parasite Plasmodium falciparum: Inhibition of histone acetylation and generation of reactive oxygen species. Antimicrob Agents Chemother. 2007; 51(2):488–94.

  • Han S, Yang Y. Antimicrobial activity of wool fabric treated with curcumin. Dyes Pigments. 2005 Feb; 64(2): 157–61.

  • Rudrappa T, Bais HP. Curcumin, a known phenolic from Curcuma longa, attenuates the virulence of Pseudomonas aeruginosa PAO1 in whole plant and animal pathogenicity models. J Agr Food Chem. 2008 Mar 26; 56(6):1955–62.

  • Goel A, Kunnumakkara AB, Aggarwal BB. Curcumin as ‘‘Curecumin’’: From kitchen to clinic. Biochem Pharmacol. 2008 Feb 15; 75(4): 787– 809.

  • Kothari V, Seshadri S, Mehta P. Fractionation of antibacterial extracts of Syzygium cumini (Myrtaceae) seeds. Res Biotechnol. 2011; 2(6):53–63.

  • Khan KH. Roles of Emblica officinalis in Medicine - A Review. Bot Res Int. 2009; 2(4): 218–28.

  • Almeida LSB, Murata RM, Yatsuda R, Dos Santos MH, Nagem TJ, Alencar SM, Koo H, Rosalen PL. Antimicrobial activity of Rheedia brasiliensis and 7-epiclusianone against Streptococcus mutans. Phytomed. 2008; 15: 886–91. DOI. 10.1016/j. phymed. 2007.12.003

  • Islam TH, Azad AHB, Akter S, Datta S. Antimicrobial activity of medicinal plants on Streptococcus mutans, A causing agent of dental caries. Int J Eng Res Technol. 2012 Dec; 1(10):1–6.

  • Larsen T, Fiehn NE, Ostergaard E. The susceptibility of dental plaque bacteria to the herbs included in longa vital. Microb Ecol Health D. 1996; 9(3): 91–5.

  • Jebashree HS, Kingsley SJ, Sathish ES, Devapriya D. Antimicrobial activity of few medicinal plants against clinically isolated human cariogenic pathogens-an in vitro study. ISRN Dent. 2011 Jun 8; 2011:1–6. DOI. 10.5402/2011/541421

  • Prabu GR, Gnanamani A, Sadulla S. Guaijaverin–a pl-ant flavonoid as potential antiplaque agent against Stre-ptococcus mutans. J App Microbiol. 2006; 101(2): 487–95.

  • Islam B, Khan SN, Haque I, Alam M, Mushfiq M, Khan AU. Novel anti-adherence activity of mulberry leaves: inhibition of Streptococcus mutans biofilm by 1-deoxynojirimycin isolated from Morus alba. J Antimicrob Chemoth. 2008 Jun 18; 62(4):751–7.

  • Dworkin M, editors. The prokaryotes. 3rd ed. New York: Springer; 2006.

  • Hatch RA, Schiller NL. Alginate lyase promotes diffusion of aminoglycosides through the extracellular polysaccharide of mucoid Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1998 Apr; 42(4):974–7.

  • Donlan RM, Costerton JW. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev. 2002 Apr; 15(2):167–93.

  • Al-Fattani, Douglas LJ. Penetration of Candida biofilms by antifungal agents. Antimicrob Agents Chemother. 2004 Sept; 48(9):3291–7.

  • Al-Sohaibani S, Murugan K. Anti-biofilm activity of Salvadora persica on cariogenic isolates of Streptococcus mutans: in vitro and molecular docking studies. Biofouling. 2012 Jan; 28(1):29–38.

  • Naidoo R, Patel M, Gulube Z, Fenyvesi I. Inhibitory activity of Dodonaea viscose var. angustifolia extract against Streptococcus mutans and its biofilm. J Ethnopharmacol, 2012 Oct 31; 144(1):171–4.

  • Marsh PD, Bradshaw DJ. Microbiological effects of new agents in dentifrices for plaque control. Int Dent J. 1993 Aug; 43(4):399–406.

  • Wilson M., Patel H, Noar JH. Effect of chlorhexidine on multi-species biofilms. Curr Microbiol. 1998 Jan; 36(1):13–18.

  • Madigan MT, Martinko JM, Dunlap PV, Clark DP. Brock biology of microorganisms. U.S.: Pearson Benjamin CummingsTM; 2009.

  • Olson ME, Ceri H, Morck DW, Buret AG, Read RR. Biofilm bacteria: formation and comparative susceptibility to antibiotics. Can J Vet Res. 2002 Apr; 66(2):86–92.

  • Roberts ME, Stewart PS. Modelling protection from antimicrobial agents in biofilms through the formation of persister cells. Microbiology. 2004 Jan; 151(1):75–80.

  • Silva NB, Alexandria AK, Lima A, Claudino LV, Carneiro TF, Costa AC, Valença AM, Cavalcanti AL. In vitro antimicrobial activity of mouth washes and herbal products against dental biofilm-forming bacteria. Contemp Clin Dent. 2012; 3(3):302–5.

  • Aneja KR, Joshi R, Sharma C. The antimicrobial potential of ten often used mouthwashes against four dental caries pathogens. Jundishapur J Microbiol, 2010 Jan; 3(1): 15–27.


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  • Tamarindus indica (Cesalpiniaceae), and Syzygium cumini (Myrtaceae) Seed Extracts can Kill Multidrug Resistant Streptococcus mutans in Biofilm

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Authors

Ina Patel
Institute of Science, Nirma University, S-G Highway, Ahmedabad, 382481, India
Vaibhavi Patel
Institute of Science, Nirma University, S-G Highway, Ahmedabad, 382481, India
Asha Thakkar
Institute of Science, Nirma University, S-G Highway, Ahmedabad, 382481, India
Vijay Kothari
Institute of Science, Nirma University, S-G Highway, Ahmedabad, 382481, India

Abstract


Extracts of Emblica officinalis seeds prepared by Microwave Assisted Extraction (MAE) method were evaluated for their antimicrobial property against planktonic form of certain human/plant pathogenic microbes. Additionally, seed extracts of E. officinalis, Tamarindus indica, Manilkara zapota, Phoenix sylvestris, Syzygium cumini, and selected phytocompounds were tested against multi-drug resistant Streptococcus mutans (a major pathogen associated with human dental caries) in its planktonic as well as biofilm form. Ability of these extracts to eradicate and kill S. mutans biofilm was investigated. E. officinalis extracts exerted bactericidal action against S. mutans, Pseudomonas aeruginosa, and Vibrio cholerae. Acetone extract of S. cumini, and curcumin were able to inhibit S. mutans at appreciably low concentrations of 50 μg/mL and 20 μg/mL respectively. T. indica and S. cumini seed extracts were able to kill ≥ 80% cells of S. mutans in biofilm, in the concentration range of 500-1000 μg/mL. These extracts were able to achieve ≥ 95% killing of S. mutans biofilm at concentrations ranging from 600-2000 μg/mL. Ability of the potent extracts to kill S. mutans biofilm did not seem to be much dependent on eradication of the biofilm. Extraction efficiency was found to have a good correlation with antibacterial activity.

Keywords


Antibacterial, Biofilm, Drug-resistance, Microwave Assisted Extraction (MAE), Minimum Inhibitory Concentration (MIC), Emblica officinalis

References