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Assessment of Testosterone Biogenesis Enhancement by a Lupeol Derivative Separated from Seeds of Hygrophila spinosa T. Ander in vitro


Affiliations
1 Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, India
2 Department of Pharmaceutical Chemistry and Analysis, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, India
 

The present study aimed towards assessing the isolated lupeol derivative in vitro for testosterone biogenesis enhancement by the action on isolated rat leydig cells. Studies were carried out to separate a novel bioactive molecule from an Indian medicinal plant, Hygrophila spinosa, used as an aphrodisiac and spermatogenic for years. Unsaponifiable bioactive fraction, prepared from seeds of the plant, was subjected to isolation of phytoconstituents by column and flash chromatography. The isolated compound was characterized by spectral studies as lupeol methyl ether. Later, it was subjected to in vitro studies using isolated leydig cells from rat for its action. Different amounts of isolated compound were incubated with leydig cells for 3 hours and concentration of testosterone after incubation was considered as a parameter. Dehydroepiandrosterone was used as positive control in the studies. The results indicated that the isolated compound was active and enhanced the testosterone biogenesis, as evident from increased amount of testosterone found in a medium treated with compound. This was the first report included isolation, chemical characterization and biological activity of Lupeol methyl ether from Hygrophila spinosa T. Ander.

Keywords

Hygrophila spinosa, Lupeol, Leydig Cells, Steroids, Tri-terpenoids, Testosterone.
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  • Assessment of Testosterone Biogenesis Enhancement by a Lupeol Derivative Separated from Seeds of Hygrophila spinosa T. Ander in vitro

Abstract Views: 55  |  PDF Views: 38

Authors

Niraj Vyas
Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, India
Kanan Gamit
Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, India
Manan Raval
Department of Pharmacognosy and Phytochemistry, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, India
Samir Patel
Department of Pharmaceutical Chemistry and Analysis, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, Charusat Campus, Changa, Anand – 388421, Gujarat, India

Abstract


The present study aimed towards assessing the isolated lupeol derivative in vitro for testosterone biogenesis enhancement by the action on isolated rat leydig cells. Studies were carried out to separate a novel bioactive molecule from an Indian medicinal plant, Hygrophila spinosa, used as an aphrodisiac and spermatogenic for years. Unsaponifiable bioactive fraction, prepared from seeds of the plant, was subjected to isolation of phytoconstituents by column and flash chromatography. The isolated compound was characterized by spectral studies as lupeol methyl ether. Later, it was subjected to in vitro studies using isolated leydig cells from rat for its action. Different amounts of isolated compound were incubated with leydig cells for 3 hours and concentration of testosterone after incubation was considered as a parameter. Dehydroepiandrosterone was used as positive control in the studies. The results indicated that the isolated compound was active and enhanced the testosterone biogenesis, as evident from increased amount of testosterone found in a medium treated with compound. This was the first report included isolation, chemical characterization and biological activity of Lupeol methyl ether from Hygrophila spinosa T. Ander.

Keywords


Hygrophila spinosa, Lupeol, Leydig Cells, Steroids, Tri-terpenoids, Testosterone.

References





DOI: https://doi.org/10.18311/jnr%2F2021%2F26314