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Antibacterial and in vitro Anticancer Study of Methanol Extracts of Clitoria ternatea Leaves


Affiliations
1 Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode, India
 

Clitoria ternatea is often known as butterfly pea plant by the natives and is one of the major plants used in Ayurveda and traditional medicine worldwide. It is also used in food and cosmetic industries for various purposes. In this study, methanol leaf extract of Clitoria ternatea was studied for its antibacterial and in vitro anticancer study against human promyelocytic leukemia cells (HL60). Secondary metabolites like alkaloids, phlobatannin, triterpenoids, flavonoids, lipids, steroids, terpenoids, tannins and glycosides are identified in the extract by phytochemical screening. Leaf extract showed the presence of ethers and carboxylic acid groups (GCMS analysis). The leaf extract showed resistance against Salmonella typhi. Thus, from this study it can be concluded Clitoria ternatea leaves methanol extract has bioactive compounds showing anticancer activity against HL60 cells.

Keywords

Antibacterial Activity, Anticancer Activity, Clitoria ternatea, GC-MS Analysis, Phytochemical Screening, MTT Assay.
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  • Antibacterial and in vitro Anticancer Study of Methanol Extracts of Clitoria ternatea Leaves

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Authors

Arunava Das
Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode, India
G. Shanmuga Priya
Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode, India
S. Soundariya
Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode, India
P. Deepesh
Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode, India
Aileen Rose Edwin
Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode, India
E. Vihashinee
Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode, India
A. Rubiga
Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode, India
S. Megavarthini
Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode, India
R. Eswaran
Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode, India
J. Bindhu
Molecular Diagnostics & Biomolecule Characterization Lab, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam – 638401, Erode, India

Abstract


Clitoria ternatea is often known as butterfly pea plant by the natives and is one of the major plants used in Ayurveda and traditional medicine worldwide. It is also used in food and cosmetic industries for various purposes. In this study, methanol leaf extract of Clitoria ternatea was studied for its antibacterial and in vitro anticancer study against human promyelocytic leukemia cells (HL60). Secondary metabolites like alkaloids, phlobatannin, triterpenoids, flavonoids, lipids, steroids, terpenoids, tannins and glycosides are identified in the extract by phytochemical screening. Leaf extract showed the presence of ethers and carboxylic acid groups (GCMS analysis). The leaf extract showed resistance against Salmonella typhi. Thus, from this study it can be concluded Clitoria ternatea leaves methanol extract has bioactive compounds showing anticancer activity against HL60 cells.

Keywords


Antibacterial Activity, Anticancer Activity, Clitoria ternatea, GC-MS Analysis, Phytochemical Screening, MTT Assay.

References





DOI: https://doi.org/10.18311/jnr%2F2020%2F24381