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Efficacy of Bee Honey on Wound Healing:Split Skin Graft with Hyper-Granulation Tissue


Affiliations
1 Medical Microbiology, Princess Nourah Bint Abdulrahman University, College of Nursing, Department of Medical Surgical, Riyadh, Saudi Arabia
2 Clinical Surgery, MD Plastic Surgery and Reconstructive Surgery, Khartoum North Teaching Hospital, Surgery and Plastic Surgery Unit, Khartoum, Sudan
 

The present prospective study was aimed to evaluate the effectiveness of topical application of bee honey on wound healing with hyper granulation tissue of infected split skin graft. Methods: A 27 years old female was admitted to Khartoum North Teaching Hospital plastic surgery unit with painful swelling of the right buttock with localized heat, which was proved as a pyogenic abscess. Swabs were taken from the wound for isolation, identification and viable bacterial count. The abscess was drained under general anesthesia and dressed with saline and MEBO ointment (combination of honey and herbs). Skin graft was performed. After 2 weeks of MEBO dressing, the wound was infected and hyper granulation tissue was observed. Daily application of honey was used instead of MEBO ointment. Results: Isolated organism was identified as Staphylococcus aureus. In vitro antibacterial test of honey against the isolated organism exhibited strong antibacterial activity. After one week of honey dressing, bacterial growth was inhibited and finally the wound was free from S. aureus. After 2 weeks of honey dressing, wound healing with reduced, hyper-granulation tissue was found. Conclusion: Topical application of honey dressing on wound with hyper granulation tissue for 3 weeks resulted in clean sterile wound with healthy granulation tissue and prompt graft was observed. Complete wound healing was achieved and hyper granulation tissue was reduced without surgical interference.

Keywords

Honey Dressing, Split Skin Graft with Hyper-Granulation Tissue, Wound Healing.
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  • Dunford C. Hypergranulation tissue. Journal of Wound Care. 1999; 8:506–7 https://doi.org/10.12968/jowc.1999.8.10.26222. PMid:10827655
  • Moody MN, Landau JM, Goldberg LH, Marquez D, Vergilis-K. 2011 IJ. 595 nm long pulsed dye laser with a hydrocolloid dressing for the treatment of hypergranulation tissue on the scalp in postsurgical defects. Dermatol Online Journal. 2011; 17:2.
  • Pokharel RP, Maeda K, Yamamoto T, Noguchi K, Iwai Y, Nakamura H, et al. Expression of vascular endothelial growth factor in exuberant tracheal granulation tissue in children. The Journal of pathology. 1999; 188:82–6. https://doi.org/10.1002/(SICI)1096-9896(199905)188:1<82::AIDPATH324>3.0.CO;2-4
  • Vuolo J. Hypergranulation: Exploring possible management options. British Journal of Nursing. 2010; 19:S4,S6–S8. https://doi.org/10.12968/bjon.2010.19.Sup2.47244. PMid:20335928
  • Young T. Common problems in wound care: Overgranulation. British Journal of Nursing. 1995; 4:169–70. https://doi.org/10.12968/bjon.1995.4.3.169
  • Falanga V, Zitelli JA, Eaglstein WH. Wound healing. The Journal of the American Academy of Dermatology. 1988; 19:559–63. https://doi.org/10.1016/S0190-9622(88)80317-7
  • Gethin G. The significance of surface pH in chronic wounds. Wounds UK. 2007; 3:52–6.
  • Singer AJ, Clark RA. Cutaneous wound healing. The New England Journal of Medicine. 1999; 341:738–46. https://doi.org/10.1056/NEJM199909023411006. PMid:10471461
  • Kaufman T, Berger J. Topical pH and burn wound healing: A review. Beyond occlusion: Wound care proceedings. Royal Society of Medicine Services Limited Israel. 1988:55–9.
  • Tsukada K, Tokunaga K, Iwama T, Mishima Y. The pH changes of pressure ulcers related to the healing process of wounds. Wounds. 1992; 4:16–20
  • Pokharel RP, Maeda K, Yamamoto T, Noguchi K, Iwai Y, Nakamura H. Expression of vascular endothelial growth factor in exuberant tracheal granulation tissue in children. The Journal of Pathology. 1999; 188:82–6. https://doi.org/10.1002/(SICI)1096-9896(199905)188:1<82::AIDPATH324> 3.0.CO;2-4
  • Gethin G, Cowman TS, Conroy RM. The impact of Manuka honey dressings on the surface pH of chronic wounds. International Wound Journal. 2008; 5:185–94. https://doi.org/10.1111/j.1742-481X.2007.00424.x. PMid:18494624
  • Wilson IA, Henry M, Quill RD, Byrne PJ. The pH of varicose ulcer surfaces and its relationship to healing. VASA. 1979; 8:339–42.
  • Romanelli M, Schipani E, Piaggesi A, Barachini P. Evaluation of surface ph on venous leg ulcers under Allevyn Dressings. London: Royal Society of Medicine Press; 1997.
  • Weller R, Price R, Ormerod A, Benjamin N, Leifert C. Antimicrobial effect of acidified nitrite on dermatophyte fungi, Candida and bacterial skin pathogens. Journal of Applied Microbiology. 2001; 90:648–52. https://doi.org/10.1046/j.1365-2672.2001.01291.x. PMid:11309079
  • Weller R, Finnen MJ. The effects of topical treatment with acidified nitrite on wound healing in normal and diabetic mice. Nitric Oxide - Biology and Chemistry. 2006; 15:395–9. https://doi.org/10.1016/j.niox.2006.04.002. PMid:16731016
  • Ahmed AK, Hoekstra MJ, Hage J, Karim RB. Honey-medicated dressing: Transformation of an ancient remedy into modern therapy. Annals of Plastic Surgery. 2003; 50:143–8. https://doi.org/10.1097/01.SAP.0000032306.44107.C1. PMid:12567050
  • AL-Hasani H, Hussein M. Study antibacterial activity of honey against some common species of pathogenic bacteria. Iraqi Journal of Science. 2018; 59(1A):30–7. https://doi.org/10.24996/ijs.2018.59.1A.5
  • Gill R, Poojar B, Bairy LK, Praveen KSE. Comparative evaluation of wound healing potential of manuka and acacia honey in diabetic and nondiabetic rats. Journal of Pharmacy and Bioallied Sciences. 2019; 11(2):116–26. https://doi.org/10.4103/JPBS.JPBS_257_18 PMid:31148887 PMCid:PMC6537647
  • Lusby PE, Coombes A, Wilkinson JM. Honey: A potent agent for wound healing? Journal of Wound, Ostomy and Continence Nursing. 2002; 29:295-300. https://doi.org/10.1097/00152192200211000-00008
  • Auguskani JPL. study to assess the chemical composition and antibacterial properties of honey. Biomedical Research. 2018; 29(19):3584–9. https://doi.org/10.4066/biomedicalresearch.29-18-919
  • Mama M, Teshome T, Detamo J. Antibacterial activity of honey against methicillin-resistant staphylococcus aureus: A laboratory-based experimental study. International Journal of Microbiology. 2019:1–9. https://doi.org/10.1155/2019/7686130. PMid:31073310 PMCid:PMC6470442
  • Gill R, Poojar B, Bairy LK, Kumar SEP. Comparative evaluation of wound healing potential of manuka and acacia honey in diabetic and nondiabetic rats. Journal of Pharmacy and Bioallied Sciences. 2019; 11(2):116–26. https://doi.org/10.4103/ JPBS.JPBS_257_18. PMid:31148887 PMCid:PMC6537647
  • Febriyenti F, Lucida H, Almahdy A, Alfikriyah I, Hanif M. Wound-healing effect of honey gel and film. Journal of Pharmacy and Bioallied Sciences. 2019 Apr-Jun; 11(2):176–80. https://doi.org/10.4103/JPBS.JPBS_184_18. PMid:31148895 PMCid:PMC6537643
  • Nasrin T, Abbas H, Gholamreza H, Rouini MR, Manayi A. Synergistic effect of honey and propolis on cutaneous wound healing in rats. Azadeh. Acta Medica Iranica. 2016; 54(4):233–9.
  • Yang KL. The use of honey in the treatment of chilblains, nonspecific ulcers, and small wounds. Chinese Medical Journal. 1944; 62:55–60.
  • Subrahmanyam M. Honey dressing versus boiled potato peel in the treatment of burns: A prospective randomized study. Burns. 1996; 22(6):491–3. https://doi.org/10.1016/0305-4179(96)00007-1
  • Hananeh WM, Ismail ZB, Alshehabat MA, Mahmoud AA, Ali JH. Effects of Sidr honey on second-intention healing of contaminated full-thickness skin wounds in healthy dogs. Bulletin of the Veterinary Institute in Pulawy. 2015; 59(3):433–9. https://doi.org/10.1515/bvip-2015-0063
  • Shaikh, Abdul Razaque; Ali, Syed Asad; Tahir, Syed Mohammad; Memon, Riaz; Memon, Abdul Salam. Role of Honey in Wounds Infected by MRSA in a Teaching Hospital. Annals of Pakistan Institute of Medical Sciences. 2016; 12(4):267–70.
  • Suryaprakash A, Tejaswini V, Girish K, Vikram S. Efficacy of honey dressing versus mechanical debridement in healing of ulcers with biofilms a comparative study. Journal of Krishna Institute of Medical Sciences University (JKIMSU). 2018 AprJun; 7(2):49–55.
  • Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology. 1966 Apr; 45(4):493–6. https://doi.org/10.1093/ajcp/45.4_ts.493. PMid:5325707
  • Garrod LP, Lambert HP, O’Grady F. Antibiotics and Chemotherapy. 5th ed. Churchill Livingstone, New York; 1988.
  • Liguori PA, Peters KL, Bowers JM. Combination of negative pressure wound therapy and acoustic pressure wound therapy for treatment of infected surgical wounds: A case series. Ostomy Wound Manage. 2008; 54(5):50–3.
  • Fukuda M, Kobayashi K, Hirono Y, Miyagawa M, Ishida T, Ejiogu EC. Jungle honey enhances immune function and antitumor activity. Evidence-Based Complementary and Alternative Medicine; 2011. https://doi.org/10.1093/ecam/nen086. PMid:19141489. PMCid:PMC3137786
  • Molan PC. The evidence supporting the use of honey as a wound dressing. The International Journal of Lower Extremity Wounds. 2006; 5(1):40–54. https://doi.org/10.1177/1534734605286014. PMid:16543212
  • Majtan J, Kumar P, Majtan T, Walls AF, Klaudiny J. Effect of honey and its major royal jelly protein 1 on cytokine and MMPmRNA transcripts in human keratinocytes. Experimental Dermatology. 2010; 19:e73–e9. https://doi.org/10.1111/j.1600-0625.2009.00994.x. PMid:19845754
  • Molan PC. The evidence and the rationale for the use of honey as a wound dressing. Wound Practice & Research. 2011; 19:204–20.
  • Molan PC. The role of honey in the management of wounds. Journal of Wound Care. 1999; 8:415–18. https://doi.org/10.12968/jowc.1999.8.8.25904. PMid:10808853
  • Cavanagh D, Beazley J, Ostapowicz F. Radical operation for carcinoma of the vulva. A new approach to wound healing. Journal of Obstetrics and Gynaecology of the British Commonwealth. 1970; 77:1037–1040. https://doi.org/10.1111/j.1471-0528.1970.tb03455.x. PMid:5483415
  • Nagi AA, Amghalia E, Shamsudin MN, Abdullah R, Mohammed R, Sekawi Z. Antibacterial activity of honey against methicillin resistant Staphylococcus aureus. Research Journal of Biological Sciences. 2009; 4(8):943–47.
  • Molan P, Rhodes T. Honey: A biological wound dressing. Wounds, 2015; 27(6):141–5.
  • Armstong S, Otis GW. The antibacterial properties of honey. Bee Culture. 1995; 123(9):500–50.
  • Vyhlídalová D, Kozáková R, Zeleníková R. Management of non-healing wounds with honey dressings. Central European Journal of Nursing and Midwifery. 2018; 9(3):880–8. https://doi.org/10.15452/CEJNM.2018.09.0019

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  • Efficacy of Bee Honey on Wound Healing:Split Skin Graft with Hyper-Granulation Tissue

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Authors

Mahasin Wadi
Medical Microbiology, Princess Nourah Bint Abdulrahman University, College of Nursing, Department of Medical Surgical, Riyadh, Saudi Arabia
Talal Geregandi
Clinical Surgery, MD Plastic Surgery and Reconstructive Surgery, Khartoum North Teaching Hospital, Surgery and Plastic Surgery Unit, Khartoum, Sudan

Abstract


The present prospective study was aimed to evaluate the effectiveness of topical application of bee honey on wound healing with hyper granulation tissue of infected split skin graft. Methods: A 27 years old female was admitted to Khartoum North Teaching Hospital plastic surgery unit with painful swelling of the right buttock with localized heat, which was proved as a pyogenic abscess. Swabs were taken from the wound for isolation, identification and viable bacterial count. The abscess was drained under general anesthesia and dressed with saline and MEBO ointment (combination of honey and herbs). Skin graft was performed. After 2 weeks of MEBO dressing, the wound was infected and hyper granulation tissue was observed. Daily application of honey was used instead of MEBO ointment. Results: Isolated organism was identified as Staphylococcus aureus. In vitro antibacterial test of honey against the isolated organism exhibited strong antibacterial activity. After one week of honey dressing, bacterial growth was inhibited and finally the wound was free from S. aureus. After 2 weeks of honey dressing, wound healing with reduced, hyper-granulation tissue was found. Conclusion: Topical application of honey dressing on wound with hyper granulation tissue for 3 weeks resulted in clean sterile wound with healthy granulation tissue and prompt graft was observed. Complete wound healing was achieved and hyper granulation tissue was reduced without surgical interference.

Keywords


Honey Dressing, Split Skin Graft with Hyper-Granulation Tissue, Wound Healing.

References





DOI: https://doi.org/10.18311/jnr%2F2020%2F24172