Journal of Natural Remedies

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In vitro Evaluation of Crude Extracts and Isolated Compounds from Goniothalamus rongklanus and Goniothalamus latestigma for Bioactive Properties


Affiliations
1 Laboratory of Natural Products, Center of Excellence for Innovation in Chemistry, Lampang Rajabhat University, 52100 Lampang, Thailand
2 The Forest Herbarium, Department of National Park, Wildlife and Plant Conservation, Ministry of Natural Resources and Environment, 10900 Bangkok, Thailand
 

The genus Goniothalamus belongs to the Annonaceae family. The pure compounds from Goniothalamus rongklanus and Goniothalamus latestigma were isolated by repeated column chromatography. The structures were recognized by NMR spectral methods. The chemical compounds of ethyl acetate extract (EtOAc) from the stems of G. rongklanus were identified as goniotriol (1) and stigmasterol glucoside (2). The EtOAc leaves extract of G. latestigma yielded a compound, which was identified as pinocembrin (3). The ethyl acetate (EtOAc) and methanol (MeOH) extract were tested for anti-HIV-1 RT and cytotoxic activities against P-388, KB, HT 29, MCF-7, A 549, ASK and CL cell lines. The EtOAc and MeOH extract of G. rongklanus showed evidence of anti-HIV-1 RT inhibition at 76.44 and 88.48 %, respectively. The EtOAc extract of G. rongklanus and G. latestigma showed cytotoxic activities on KB and HT 29 with an ED50 at <4 μg/mL. In addition, antibacterial study on extracts and isolated compounds was also performed. Antibacterial study was evaluated using nine strains (Staphylococcus aureus, Enterobacter aerogenes, Escherichia coli 0157: H7, Escherichia coli (ETEC), Escherichia coli (EPEC), Proteus mirabilis, Salmonella typhimuriam, Shigella flexneri and Vibrio cholera) by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) method. Goniotriol was the most effective antibacterial with a MIC in the range <0.16 - 0.6 mg/mL and MBC in the range 0.3 - >5 mg/mL. The antibacterial activity of goniotriol has been reported for the first time.

Keywords

Antibacterial Activity, Anti-HIV-1 RT, Cytotoxicity, Goniothalamus rongklanus, Goniothalamus lagestima.
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  • In vitro Evaluation of Crude Extracts and Isolated Compounds from Goniothalamus rongklanus and Goniothalamus latestigma for Bioactive Properties

Abstract Views: 379  |  PDF Views: 140

Authors

Nutthapol Funnimid
Laboratory of Natural Products, Center of Excellence for Innovation in Chemistry, Lampang Rajabhat University, 52100 Lampang, Thailand
Wilart Pompimon
Laboratory of Natural Products, Center of Excellence for Innovation in Chemistry, Lampang Rajabhat University, 52100 Lampang, Thailand
Narong Nuntasaen
The Forest Herbarium, Department of National Park, Wildlife and Plant Conservation, Ministry of Natural Resources and Environment, 10900 Bangkok, Thailand

Abstract


The genus Goniothalamus belongs to the Annonaceae family. The pure compounds from Goniothalamus rongklanus and Goniothalamus latestigma were isolated by repeated column chromatography. The structures were recognized by NMR spectral methods. The chemical compounds of ethyl acetate extract (EtOAc) from the stems of G. rongklanus were identified as goniotriol (1) and stigmasterol glucoside (2). The EtOAc leaves extract of G. latestigma yielded a compound, which was identified as pinocembrin (3). The ethyl acetate (EtOAc) and methanol (MeOH) extract were tested for anti-HIV-1 RT and cytotoxic activities against P-388, KB, HT 29, MCF-7, A 549, ASK and CL cell lines. The EtOAc and MeOH extract of G. rongklanus showed evidence of anti-HIV-1 RT inhibition at 76.44 and 88.48 %, respectively. The EtOAc extract of G. rongklanus and G. latestigma showed cytotoxic activities on KB and HT 29 with an ED50 at <4 μg/mL. In addition, antibacterial study on extracts and isolated compounds was also performed. Antibacterial study was evaluated using nine strains (Staphylococcus aureus, Enterobacter aerogenes, Escherichia coli 0157: H7, Escherichia coli (ETEC), Escherichia coli (EPEC), Proteus mirabilis, Salmonella typhimuriam, Shigella flexneri and Vibrio cholera) by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) method. Goniotriol was the most effective antibacterial with a MIC in the range <0.16 - 0.6 mg/mL and MBC in the range 0.3 - >5 mg/mL. The antibacterial activity of goniotriol has been reported for the first time.

Keywords


Antibacterial Activity, Anti-HIV-1 RT, Cytotoxicity, Goniothalamus rongklanus, Goniothalamus lagestima.

References





DOI: https://doi.org/10.18311/jnr%2F2019%2F23766