Journal of Natural Remedies

Open Access Open Access  Restricted Access Subscription Access

Comparative Study on Myelotoxic and Antineoplastic Action of Synadenium umbellatum, Vitis vinifera and Resveratrol


Affiliations
1 Laboratorio de Analises Clínicas da, Av Principe de Gales, 821, CEP 09060-650 Santo Andre, Sao Paulo, Brazil
2 Departamento de Ciencias Farmaceuticas da Universidade Federal de Sao Paulo, Diadema, Sao Paulo, Brazil
3 Departamento de Farmacologia da Farmaceuticas da UNICAMP, Campinas, Sao Paulo, Brazil
 

Cancer is one of the pathologies that most challenge medicine, not only because of the complexity of its development, but also because of the difficulty of treatment that is not always effective. Due to the significant toxicity and adverse effects profile presented by the main chemotherapeutic agents used to treat neoplasm, there is a constant interest in the search for new drugs that may be a more effective alternative. Therefore, the search for new compounds of plant origin becomes an interesting tool for the discovery of drugs with antitumor activity. Synadenium umbellatum is a plant native to tropical Africa known as “cola-nota”, “hazel”, “cancerous”, “miraculous”, among others. The plant is used by the Brazilian population as having anti-inflammatory, analgesic, antineoplastic properties, among others. However, the literature is lacking on reports of the toxicity of Synadenium umbellatum macerate. Resveratrol is a polyphenolic compound found in different plant species, mainly in grape species (Vitis) and their seeds. Vitis vinifera is a species of grape easily found in several regions, also containing a high concentrations of phenolic compounds, including resveratrol in its fruits, leaves and seeds. Therefore, this study was aimed to evaluate the myelotoxicity of macerate, extract and resveratrol and their antitumor activity in Balb/c mice with Ehrlich tumor. The hematological evaluation was obtained by flow cytometry and the tumors were measured using a pachymeter and the tumor masses by means of weighing. No statistically significant difference was observed between results from the control group and the treatment groups. It was concluded that macerate, crude extract and resveratrol did not demonstrate a myelotoxic effect and did not cause a decrease in tumor mass.

Keywords

Antineoplastics, Drug Toxicity, Phytotherapy.
Font Size

User

Notifications
JOURNAL COVERS
  

  • Siegel RL, Miller KD, Jemal A. Cancer statistics, 2017. CA: A Cancer Journal for Clinicians. 2017; 67(1):7-30. https://doi.org/10.3322/caac.21387 PMid:28055103

  • Ianchini F, Vainio H. Allium vegetables and organosulfur compounds: do they help prevent cancer? Environmental Health Perspectives. 2001; 109(9):893-902. https://doi.org/10.1289/ ehp.01109893 PMid:11673117 PMCid:PMC1240438

  • Valadares MC, Ramos LA, Rehmann FK, Sweeney NJ, Strohfeldt K, Tacke M, Queiroz LSM. Antitumour activity of [1,2-di(cyclopentadienyl)-1,2-di(p-N,Ndimethylaminophenyl)ethanediyl] titanium dichloride in xenografted Ehrlich’s ascites tumour. European Journal of Pharmacology. 2006; 534:264-70. https://doi.org/10.1016/j.ejphar.2006.01.056 PMid:16513106

  • Batista AC, Benfica PL, Martins FS, Mota MF, Paula JR, Valadares MC. Investigation of Ehrlich ascites tumor cell death mechanisms induced by Synadenium umbellatum Pax. Journal of Ethnopharmacology. 2012 Jan 31; 139(2):319-29. https://doi.org/10.1016/j.jep.2011.04.055 PMid:21549815

  • Luz LE, Paludo KS, Santos VL, Franco CR, Klein T, Silva RZ, Budel JM. Cytotoxicity of latex and pharmacobotanical study of leaves and stem of Euphorbia umbellata (Janauba). Revista Brasileira de Farmacognosia. 2015; 25(4):344-52. https://doi.org/10.1016/j.bjp.2015.07.005

  • Alves N, Azeredo FS, Bara MT, Bozinis MCV, Cunha LC, Garrote CF, De Paula JR, Goloni R, Pucci LL, Tresvenzol L, Vieira MS. Avaliacao da toxidade aguda, em ratos, do extrato etanolico das folhas de Synadenium umbellatum. Revista Brasileira de Farmacognosia. 2009; 19(2a) Joao Pessoa Abr/ Junho. https://doi.org/10.1590/S0102-695X2009000300012

  • Wang Y, Stevens VL, Shah R, Peterson JJ, Dwyer JT, Gapstur SM, Mccullough ML. Dietary Flavonoid and Proanthocyanidin Intakes and Prostate Cancer Risk in a Prospective Cohort of US Men. American Journal of Epidemiology. 2014; 179:974-86. https://doi.org/10.1093/aje/kwu006 PMid:24567173

  • Sovak M. Grape Extract, Resveratrol, and Its Analogs: A Review. Journal of Medicinal Food. 2001; 4(2). https://doi.org/10.1089/109662001300341752 PMid:12639418

  • Botan AG. Citotoxicidade e ação anti-inflamatoria in vitro dos extratos glicólicos de Morus nigra (amora), Ziziphus joazeiro (juá) e Vitis vinifera (uva). 2018.

  • Noh KT, Chae SH, Chun SH, Jung ID, Kang HK, Park YM. Resveratrol suppresses tumor progression via the regulation of indoleamine 2,3- dioxygenase. Biochemical and Biophysical Communications. 2013; 431:348-53. https://doi.org/10.1016/j.bbrc.2012.12.093 PMid:23291179

  • Udenigwe CC, Ramprasath VR, Aluko RE, Jones PJ. Potential of resveratrol in anticancer and anti-inflammatory therapy. Nutrition Reviews. 2008; 66:445-54. https://doi.org/10.1111/j.1753-4887.2008.00076.x PMid:18667005

  • Fernandes F, Ramalhosa E, Pires P, Verdial J, Valentao P, Andrade P, Bento A, Pereira JA. Vitis vinifera leavestowards bioactivity. Industrial Crops and Products. 2013; 43:434-40. https://doi.org/10.1016/j.indcrop.2012.07.031

  • Campos M, Castro NC, Cunha LC. Synadenium umbellatum: citotoxicidade e danos ao DNA de celulas da medula ossea de camundongos. Revista Brasileira de Ciencias Farmaceuticas. 2007; 43:631-8. https://doi.org/10.1590/S1516-93322007000400017

  • Conselho Nacional De Saude. Resolucao Nº 001, DE 1988, outorgada pelo Decreto nº 93.933 de 14 de janeiro de 1987.

  • Lucio EMRA, Rosalen PL, Sharapin N, Souza ARM. Avaliacao toxicologica aguda e creening hipocratico da epilsopilosina, alcaloide secundario de Pilocarpus microphyllus Stapf. Revista Brasileira de Farmacognosia. 2000; 9(10):23-5. https://doi.org/10.1590/S0102-695X2000000100003

  • Melo PR, Bezerra LSA, Vale MAAB, Canhete RFR, ChenChen L. Avaliacao da atividade mutagenica e antimutagenica do latex de Synadenium umbellatum Pax pelo teste do micronucleo em camundongos. Brazilian Journal of Biology. [online]. 2011; 71(1):169-74. https://doi.org/10.1590/S151969-842011000100024

  • Cruz LS, De Oliveira TL, Kanunfre CC, Paludo KS, Minozzo BR, Prestes AP and Khan SI. Pharmacokinetics and cytotoxic study of euphol from Euphorbia umbellata (Bruyns) Pax latex. Phytomedicine. 2018; 47:105-12. https://doi.org/10.1016/j.phymed.2018.04.055 PMid:30166094

  • Fernandes JFDN, Silva BSDS, Fontes RMS, Candido WP and Malavasi NV. Avaliacao do potencial citotoxico e mutagenico/genotoxico do latex de janauba (Synadenium grantii Hook. f., Euphorbiaceae). Revista Pan-Amazonica de Saude. 2018; 9(1):59-65. https://doi.org/10.5123/S2176-62232018000100008

  • De Souza Silva SC, Alves MA, De Sousa SA, De Souza Nogueira JR, Martins DHNL. Perfil fitoquimico, susceptibilidade antibacteriana e capacidade antioxidante das folhas de Croton urucurana Baillon (Euphorbiaceae). InfarmaCiencias Farmaceuticas. 2017; 29(3):264-70. https://doi.org/10.14450/2318-9312.v29.e3.a2017.pp264-270

  • Klaassen CD, Watkins III EJB. Absorção, Distribuição e Excreção dos Tóxicos. In: Toxicologia: A Ciência Básica dos. Tóxicos de Casaret e Doull. McGraw-Hill, 5a Ed. 2001; p. 79-100.

  • Cunha LC, Azevedo FS, Mendonca ACV, Vieira MAMS, Pucci LL, Valadares MC, Freitas HOG, Sena AAS. Avaliação da toxicidade aguda e subaguda, em ratos, do extrato etanólico das folhas e do látex de Synadenium umbelllatum Pax. Revista Brasileira de Farmacognosia. 2009; 19(2A):403-11. https://doi.org/10.1590/S0102-695X2009000300012

  • Da Silva EB et al. Synadenium umbellatum and the Ehrlich’s solid tumor treatment: Assessment of inflammatory regulators (transforming growth factor protein-β1 and tumor necrosis factor-α) gene expression, hepatotoxicity and myelotoxicity. European Journal of Oncology Pharmacy. 2019; 2(1):e9. ISSN 2032-7072. https://doi.org/10.1097/ OP9.0000000000000009 ; https://journals.lww.com/ejop/Fulltext/2019/03000/Synadenium_umbellatum_and_the_Ehrlich_s_solid.1.aspx

  • Castro GMAD. Perfil dos marcadores tumorais e sua correlação com parâmetros hematológicos. 2017.

  • Ning ANY, Yin D, Zhang HJ, Liu Z, Feng F, Hu X. Melanomainduced anemia could be rescued by Sca-1+ mesenchymal stromal cells in mice. Stem Cells and Development. 2017; 26(7):495-502. https://doi.org/10.1089/scd.2016.0139 PMid:28052733

  • Gupta M. Antitumor activity and antioxidante status of Caesalpnia bonducella against. Ehrlich ascistes carcinoma in swiss albino mice. Journal of Pharmacological Sciences. 2004; 94:177-84. https://doi.org/10.1254/jphs.94.177 PMid:14978356

  • Valadares MC. Euphorbia tirucalli L. Modulates myelopoiesis and enhances the resistance of tumor - bearing mice. Internacional Immunopharmacology. 2006; 6(2):294-9. https://doi.org/10.1016/j.intimp.2005.07.013 PMid:16399635

  • El-Azab M, Hishe H, Moustafa Y, El-Awady E-S Antiangiogenic effect of resveratrol or curcumin in Ehrlich ascites carcinoma-bearing mice. European Journal of Pharmacology. 2011; 652:7-14. https://doi.org/10.1016/j.ejphar.2010.11.008 PMid:21114990

  • Rossi M, Caruso F, Opaso C, Salciccioli J. Crystal and molecular structure of piceatannol; scavenging features of resveratrol and piceatannol on hydroxyl and peroxyl radicals and docking with transthyretin. Journal of Agricultural and Food Chemistry. 2008; 56:10557-66. https://doi.org/10.1021/jf801923j PMid:18959413

  • Diaz-Gerevini GT, Repossi G, Dain A, Tarres MC, Das UN, Eynard AR. Beneficial action of resveratrol: how and why? Nutrition. 2016; 32(2):174-8. https://doi.org/10.1016/j.nut.2015.08.017 PMid:26706021

  • Santos OJD, Sauaia Filho EN, Nascimento FRFD, Junior FCS, Fialho EMS, Santos RHP, Serra ICPB. Use of raw Euphorbia tirucalli extract for inhibition of ascitic Ehrlich tumor. Revista do Colégio Brasileiro de Cirurgiões. 2016; 43(1):18-21. https://doi.org/10.1590/0100-69912016001005 PMid:27096852 .


Abstract Views: 320

PDF Views: 197




  • Comparative Study on Myelotoxic and Antineoplastic Action of Synadenium umbellatum, Vitis vinifera and Resveratrol

Abstract Views: 320  |  PDF Views: 197

Authors

Emerson B. da Silva
Laboratorio de Analises Clínicas da, Av Principe de Gales, 821, CEP 09060-650 Santo Andre, Sao Paulo, Brazil
Camila S. Chagas
Departamento de Ciencias Farmaceuticas da Universidade Federal de Sao Paulo, Diadema, Sao Paulo, Brazil
Marina P. A. Oliveira
Departamento de Ciencias Farmaceuticas da Universidade Federal de Sao Paulo, Diadema, Sao Paulo, Brazil
Alexandre L. A. Fonseca
Laboratorio de Analises Clínicas da, Av Principe de Gales, 821, CEP 09060-650 Santo Andre, Sao Paulo, Brazil
Beatriz da C. A. Alves
Laboratorio de Analises Clínicas da, Av Principe de Gales, 821, CEP 09060-650 Santo Andre, Sao Paulo, Brazil
Izilda Bagatin
Departamento de Ciencias Farmaceuticas da Universidade Federal de Sao Paulo, Diadema, Sao Paulo, Brazil
Fabio F. Perazzo
Departamento de Ciencias Farmaceuticas da Universidade Federal de Sao Paulo, Diadema, Sao Paulo, Brazil
Paulo Rosa
Departamento de Farmacologia da Farmaceuticas da UNICAMP, Campinas, Sao Paulo, Brazil
David Feder
Laboratorio de Analises Clínicas da, Av Principe de Gales, 821, CEP 09060-650 Santo Andre, Sao Paulo, Brazil
Giuliana Petri
Laboratorio de Analises Clínicas da, Av Principe de Gales, 821, CEP 09060-650 Santo Andre, Sao Paulo, Brazil
Fernando Luiz A. Fonseca
Laboratorio de Analises Clínicas da, Av Principe de Gales, 821, CEP 09060-650 Santo Andre, Sao Paulo, Brazil

Abstract


Cancer is one of the pathologies that most challenge medicine, not only because of the complexity of its development, but also because of the difficulty of treatment that is not always effective. Due to the significant toxicity and adverse effects profile presented by the main chemotherapeutic agents used to treat neoplasm, there is a constant interest in the search for new drugs that may be a more effective alternative. Therefore, the search for new compounds of plant origin becomes an interesting tool for the discovery of drugs with antitumor activity. Synadenium umbellatum is a plant native to tropical Africa known as “cola-nota”, “hazel”, “cancerous”, “miraculous”, among others. The plant is used by the Brazilian population as having anti-inflammatory, analgesic, antineoplastic properties, among others. However, the literature is lacking on reports of the toxicity of Synadenium umbellatum macerate. Resveratrol is a polyphenolic compound found in different plant species, mainly in grape species (Vitis) and their seeds. Vitis vinifera is a species of grape easily found in several regions, also containing a high concentrations of phenolic compounds, including resveratrol in its fruits, leaves and seeds. Therefore, this study was aimed to evaluate the myelotoxicity of macerate, extract and resveratrol and their antitumor activity in Balb/c mice with Ehrlich tumor. The hematological evaluation was obtained by flow cytometry and the tumors were measured using a pachymeter and the tumor masses by means of weighing. No statistically significant difference was observed between results from the control group and the treatment groups. It was concluded that macerate, crude extract and resveratrol did not demonstrate a myelotoxic effect and did not cause a decrease in tumor mass.

Keywords


Antineoplastics, Drug Toxicity, Phytotherapy.

References





DOI: https://doi.org/10.18311/jnr%2F2019%2F23383