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The Effect of 70% Ethanolic Extract of Dayak Onion Bulbs and Cowpea on Cardiovascular Parameters of Hypoestrogen Model Rats


Affiliations
1 Department Pharmacology and Toxicology, Universitas Indonesia, Depok, 16424, West Java, Indonesia
 

Introduction: Eleutherinol has strong affinity to bind with Estrogen Receptor alpha (ER-α) and can be found in Dayak onion bulbs (Eleutherine bulbosa (Mill.) Urb.). The cowpea (Vigna unguiculata (L.) Walp) contain Daidzein that acts on estrogen receptors beta (ER-β). This study was aimed to investigate the effect of both extracts on ER-α and ER-β by estimating the blood pressure and serum lipid profile of hypoestrogen model rats. Material and Methods: Thirty-six of female Sprague-Dawley were randomly assigned to eight groups as followed, SHAM, OVX, RAL (Raloxifene 1 mg/200 g BW), Cowpea 100 mg/200 g BW with Dayak onion bulbs: 36 mg/200 g BW (D1); 18 mg/200 g BW (D2); 9 mg/200 g BW (D3); 4.5 mg/200 g BW (D4) and single dose of cowpea 100 mg/200 g BW (D5). All groups, except the SHAM,were ovariectomized to obtain the conditions of hypoestrogen. The extracts were given orally for 28 days. Blood pressure and lipid profile were measured after 28 days of treatment. Results: Combination of extracts at D2 (18 mg/200 g BW Dayak onion bulbs and 100 mg/200 g BW cowpea) significantly decreased blood pressure and serum lipid profile of hypoestrogen model rats. The combination extracts was also better than a single dose of 100 mg/200 g BW cowpea. Conclusions: The results indicated that a combination extract of Dayak onion bulbs and cowpea could be used as a drug for menopause woman in the future.

Keywords

Blood Pressure, Cowpea, Dayak Onion, Hypoestrogen, Lipid Profile.
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  • Reece JB, Urry LA, Cain ML, Wasserman SA, Minorsky PV, Jackson RB. Campbell Biology (9th ed.). San Fransisco: Pearson Benjamin Cummings; 2011.
  • Pfaffl MW, Lange IG, Daxenberger A, Meyer HH. Tissue-specific expression pattern of Estrogen Receptors (ER): Quantification of ER alpha and ER beta mRNA with real-time RT-PCR. APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica. 2001; 109(5): 345–55. PMid: 11478682. https://doi.org/10.1034/j.1600-0463.2001.090503.x
  • Paterni I, Granchi C, Katzenellenbogen JA, Minutolo F. Estrogen Receptors alpha (ERα) and beta (ERβ): Subtypeselective ligands and clinical potential. Steroids. 2014; 90: 13–29. PMid: 24971815 PMCid: PMC4192010 https://doi.org/10.1016/j.steroids.2014.06.012
  • Foryst-Ludwig A, Clemenz M, Hohmann S, Hartge M, Sprang C, Frost N, … Kintscher U. Metabolic actions of Estrogen Receptor beta (ERβ) are mediated by a negative cross-talk with PPARγ. PLoS Genetics. 2008; 4(6). PMid: 18584035 PMCid: PMC2432036. https://doi.org/10.1371/journal.pgen.1000108
  • da Silva CC, Lazzaretti C, Fontanive T, Dartora DR, Bauereis B, Gamaro GD. Estrogen-dependent effects on behavior, lipid-profile and glycemic index of ovariectomized rats subjected to chronic restraint stress. Behavioural Processes.2014; 103: 327–33. PMid: 24496020 https://doi.org/10.1016/j.beproc.2014.01.022
  • Hargrove GM, Junco A, Wong NC. Hormonal regulation of apolipoprotein AI. Journal of Molecular Endocrinology. 1999; 22(2): 103–11. PMid: 10194513. https://doi.org/10.1677/jme.0.0220103
  • Shen M, Shi H. Sex hormones and their receptors regulate liver energy homeostasis. International Journal of Endocrinology. 2015; 2015: 1–12. PMid: 26491440 PMCid: PMC4600502. https://doi.org/10.1155/2015/294278
  • Xu B, Lovre D, Mauvais-Jarvis F. Effect of selective Estrogen Receptor modulators on metabolic homeostasis. Biochimie; 2015. p. 1–6. http://www.ncbi.nlm.nih.gov/pubmed/26133657
  • An KC. Selective Estrogen Receptor modulators. Asian Spine Journal. 2016; 10(4): 787–91. PMid: 27559463 PMCid: PMC4995266. https://doi.org/10.4184/asj.2016.10.4.787
  • Johnson I, Williamson G. Phytochemical functional foods. New York. Cambridge: Woodhead Publishing Limited; 2003. https://doi.org/10.1201/9780203506318
  • Bahtiar A, Vichitphan K, Han J. Leguminous plants in the Indonesian Archipelago: Traditional Uses and Secondary Metabolites. Natural Product Communications. 2017; 12(3): 461–472. PMid: 30549910. https://doi.org/10.1177/1934578X1701200338
  • Bahtiar A, Chumala DY. Dayak onions (Eleutherine bulbosa (Mill.) Urb.) Bulbs extracts reduce the blood pressure of ovariectomized rats. Journal of Natural Remedies. 2018; 36(3): 1016–21. https://doi.org/10.4067/S0717-95022018000301016
  • Sun MY, Ye Y, Xiao L, Rahman,K, Xia W, Zhang H. Daidzein: A review of pharmacological effects. African Journal of Traditional, Complementary and Alternative Medicines. 2016; 13(3): 117. https://doi.org/10.21010/ajtcam.v13i3.1
  • Jia M, Dahlman-Wright K, Gustafsson JA. EstrogenR alpha and beta in health and disease. Best Practice and Research: Clinical Endocrinology and Metabolism. 2015; 29(4): 557–68. PMid: 26303083. https://doi.org/10.1016/j.beem.2015.04.008
  • Pallottini V, Bulzomi P, Galluzzo P, Martini C, Marino M. Estrogen regulation of adipose tissue functions: Involvement of Estrogen Receptor isoforms. Infectious Disorders-Drug Targets (Formerly Current Drug Targets-Infectious Disorders). 2008; 8(1): 52–60. https://doi.org/10.2174/187152608784139631
  • Rodwell VW, Bender DA, Botham KM, Kennelly PJ, Weil PA. Harper’s illustrated biochemistry. Harper’s Illustrated Biochemistry (30th ed.). New York: McGraw-Hill Education LLC; 2015.

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  • The Effect of 70% Ethanolic Extract of Dayak Onion Bulbs and Cowpea on Cardiovascular Parameters of Hypoestrogen Model Rats

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Authors

Anton Bahtiar
Department Pharmacology and Toxicology, Universitas Indonesia, Depok, 16424, West Java, Indonesia
Farah Amandasari
Department Pharmacology and Toxicology, Universitas Indonesia, Depok, 16424, West Java, Indonesia

Abstract


Introduction: Eleutherinol has strong affinity to bind with Estrogen Receptor alpha (ER-α) and can be found in Dayak onion bulbs (Eleutherine bulbosa (Mill.) Urb.). The cowpea (Vigna unguiculata (L.) Walp) contain Daidzein that acts on estrogen receptors beta (ER-β). This study was aimed to investigate the effect of both extracts on ER-α and ER-β by estimating the blood pressure and serum lipid profile of hypoestrogen model rats. Material and Methods: Thirty-six of female Sprague-Dawley were randomly assigned to eight groups as followed, SHAM, OVX, RAL (Raloxifene 1 mg/200 g BW), Cowpea 100 mg/200 g BW with Dayak onion bulbs: 36 mg/200 g BW (D1); 18 mg/200 g BW (D2); 9 mg/200 g BW (D3); 4.5 mg/200 g BW (D4) and single dose of cowpea 100 mg/200 g BW (D5). All groups, except the SHAM,were ovariectomized to obtain the conditions of hypoestrogen. The extracts were given orally for 28 days. Blood pressure and lipid profile were measured after 28 days of treatment. Results: Combination of extracts at D2 (18 mg/200 g BW Dayak onion bulbs and 100 mg/200 g BW cowpea) significantly decreased blood pressure and serum lipid profile of hypoestrogen model rats. The combination extracts was also better than a single dose of 100 mg/200 g BW cowpea. Conclusions: The results indicated that a combination extract of Dayak onion bulbs and cowpea could be used as a drug for menopause woman in the future.

Keywords


Blood Pressure, Cowpea, Dayak Onion, Hypoestrogen, Lipid Profile.

References





DOI: https://doi.org/10.18311/jnr%2F2019%2F23372