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Chemical Composition, Larvicidal and Antibacterial Activity of the Essential Oil of Trachyspermum ammi Fruit


Affiliations
1 Department of Laboratory Sciences, College of Sciences and Arts at Al-Rass, Qassim University, Al-Rass, Saudi Arabia
2 Department of Laboratory Sciences, College of Sciences and Arts at Al-Rass, Qassim University, Al-Rass, Egypt
3 Department of Chemistry, College of Science and Arts at Al-Rass, Qassim University, Al-Rass, Saudi Arabia
4 Department of Pharmaceutical Analysis, Siberian state Medical University, Tomsk 634050, Russian Federation
 

Trachyspermum ammi fruits (T. ammi) are widely sold and used in the Arabian herbal markets, which requires scientific investigations about its biological activities. The GC-MS analysis of the essential oil revealed that it contains 20 compounds, of which the main components are aromatic and monocyclic terpenes. Thymol was present in the higher amounts (36.00%) followed by γ-terpinene (26.01%) and m-cymene (21.44%). The essential oil of T. ammi contains about 9.77% of bicyclic terpenes of which β-pinene is about 6.70%. The anti-bacterial activity (in vitro) was performed by disc diffusion, MIC and MBC methods. The essential oil of the fruits of T. ammi exhibited significant dose-dependent antibacterial activity against all tested microorganisms (three Gram-positive and four Gram-negative bacteria) and it was comparable to the positive control (Chloramphenicol), with a remarkable MIC and MBC values. Water extract of T. ammi exhibited promising larvicidal activity against S. littoralis and the lethal concentration, LC50 was found to be 6.21%. The current study provides some scientific support to the application of T. ammi fruits in traditional medicine and suggests it as a source of antibacterial drug and natural larvicidal agents for crops as biocontrols.

Keywords

GC-MS, MBC, MIC, Spodoptera Littoralis.
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  • Chemical Composition, Larvicidal and Antibacterial Activity of the Essential Oil of Trachyspermum ammi Fruit

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Authors

Ahmed M. H. Ali
Department of Laboratory Sciences, College of Sciences and Arts at Al-Rass, Qassim University, Al-Rass, Saudi Arabia
Emad M. Abdallah
Department of Laboratory Sciences, College of Sciences and Arts at Al-Rass, Qassim University, Al-Rass, Egypt
Adil A. H. Mujawah
Department of Chemistry, College of Science and Arts at Al-Rass, Qassim University, Al-Rass, Saudi Arabia
Elena Yu. Avdeeva
Department of Pharmaceutical Analysis, Siberian state Medical University, Tomsk 634050, Russian Federation

Abstract


Trachyspermum ammi fruits (T. ammi) are widely sold and used in the Arabian herbal markets, which requires scientific investigations about its biological activities. The GC-MS analysis of the essential oil revealed that it contains 20 compounds, of which the main components are aromatic and monocyclic terpenes. Thymol was present in the higher amounts (36.00%) followed by γ-terpinene (26.01%) and m-cymene (21.44%). The essential oil of T. ammi contains about 9.77% of bicyclic terpenes of which β-pinene is about 6.70%. The anti-bacterial activity (in vitro) was performed by disc diffusion, MIC and MBC methods. The essential oil of the fruits of T. ammi exhibited significant dose-dependent antibacterial activity against all tested microorganisms (three Gram-positive and four Gram-negative bacteria) and it was comparable to the positive control (Chloramphenicol), with a remarkable MIC and MBC values. Water extract of T. ammi exhibited promising larvicidal activity against S. littoralis and the lethal concentration, LC50 was found to be 6.21%. The current study provides some scientific support to the application of T. ammi fruits in traditional medicine and suggests it as a source of antibacterial drug and natural larvicidal agents for crops as biocontrols.

Keywords


GC-MS, MBC, MIC, Spodoptera Littoralis.

References





DOI: https://doi.org/10.18311/jnr%2F2019%2F23310