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Phytochemical Characterization, In-Vitro Cytotoxic and Antibacterial Activity of Cotula cinerea (Delile) Vis Essential Oil


Affiliations
1 University of Hamma Lakhdar El-Oued, B.P.789, 39000, Algeria
2 Department of Ecophysiology, Desert Research Center, Mathaf El-Mataria, 15753, Egypt
 

Cotula cinerea is a traditional Algerian medicinal plant that has many biological properties, such as analgesic, antiseptic and antibacterial activities. Essential oil of Cotula cinerea was subjected to chromatographical and spectroscopical studies to determine the chemical composition. The analysis highlighted that the oil contains trans thujone (51.86%), santalina triéne (10.6%), α-pinéne (2.02%), sabinene (6.17%), cineole <1.8> (5.34%), δ-terpinene (1.57%), camphor (2.63%), β-terpineol (1.39 %) and terpin-4-ol (1.73%) as the major constituents. The essential oil was tested for antibacterial activity. The oil exhibited substantial antibacterial activity against both the tested Gram negative and Gram positive bacteria. In search of better anticancer agent the essential oil of Cotula cinerea was also subjected for in-vitro cytotoxic activity on two cancerous human cell lines; colon (HCT116) and hepatic (HePG2) cancer cell lines. The results indicates that the essential oil of Cotula cinerea has a significant cytotoxic activity on the tested colon cancer cell lines with a 66.9% inhibition and a minimal inhibition (33.9%) on liver cancer cell line.

Keywords

Essential Oil Composition, GC/MS, HCT116, HePG2.
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  • Phytochemical Characterization, In-Vitro Cytotoxic and Antibacterial Activity of Cotula cinerea (Delile) Vis Essential Oil

Abstract Views: 314  |  PDF Views: 135

Authors

Ben Amor M. Larbi
University of Hamma Lakhdar El-Oued, B.P.789, 39000, Algeria
Benchikha Naima
University of Hamma Lakhdar El-Oued, B.P.789, 39000, Algeria
Eman R. Elsharkawy
Department of Ecophysiology, Desert Research Center, Mathaf El-Mataria, 15753, Egypt
N. Salah Neghmouche
University of Hamma Lakhdar El-Oued, B.P.789, 39000, Algeria

Abstract


Cotula cinerea is a traditional Algerian medicinal plant that has many biological properties, such as analgesic, antiseptic and antibacterial activities. Essential oil of Cotula cinerea was subjected to chromatographical and spectroscopical studies to determine the chemical composition. The analysis highlighted that the oil contains trans thujone (51.86%), santalina triéne (10.6%), α-pinéne (2.02%), sabinene (6.17%), cineole <1.8> (5.34%), δ-terpinene (1.57%), camphor (2.63%), β-terpineol (1.39 %) and terpin-4-ol (1.73%) as the major constituents. The essential oil was tested for antibacterial activity. The oil exhibited substantial antibacterial activity against both the tested Gram negative and Gram positive bacteria. In search of better anticancer agent the essential oil of Cotula cinerea was also subjected for in-vitro cytotoxic activity on two cancerous human cell lines; colon (HCT116) and hepatic (HePG2) cancer cell lines. The results indicates that the essential oil of Cotula cinerea has a significant cytotoxic activity on the tested colon cancer cell lines with a 66.9% inhibition and a minimal inhibition (33.9%) on liver cancer cell line.

Keywords


Essential Oil Composition, GC/MS, HCT116, HePG2.

References





DOI: https://doi.org/10.18311/jnr%2F2018%2F22380