Journal of Natural Remedies

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Effect of Thyponium flageliforme in Combination with Curcuma zedoaria and Phyllanthus niruri in Expression of Protein Estrogen Receptor and Caspase-9 on Breast Cancer Mice


Affiliations
1 Department of Chemistry, Sultan Agung Islamic University, Jawa Tengah - 50112, Indonesia
2 Department of Anatomy, Sultan Agung Islamic University, Jawa Tengah - 50112, Indonesia
3 Department of Biochemistry, Sultan Agung Islamic University, Semarang, Jawa Tengah - 50112, Indonesia
 

Introduction: Incidence of breast cancer is high and constitutes a significant cause of female mortality. One of breast cancer type is Estrogen and Progesteron Receptor (ER and PR) positive, therefore affected by estrogen and progesteron concentration. Treatment of Thyponium flageliforme in combination with Curcuma zedoaria, and Phyllanthus niruri (TCP) have been proven capable of inhibiting tumor cells proliferation. Objective: To evaluate the expression of protein estrogen receptor and caspase-9 in C3H mice were inoculated with adenocarcinoma cells. Methods: 18 mice were inoculated with one million cells of breast adenocarcinoma per mouse and assigned into three groups. TCP with dosage 85 and 125 mg were treated for 21 days to evaluate the expression of protein estrogen receptor and caspase-9 measured by immuno-histochemical staining. Results: Mann Whitney analysis demonstrated that expression of protein estrogen receptor and caspase-9 in TCP 125 was significant (p < 0.05). Conclusion: Expression of protein estrogen receptor was decreased and caspase-9 expression was increased by TCP administration.

Keywords

Breast Cancer, Caspase9, Combination of Herbal Extracts, Estrogen Receptors.
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  • Effect of Thyponium flageliforme in Combination with Curcuma zedoaria and Phyllanthus niruri in Expression of Protein Estrogen Receptor and Caspase-9 on Breast Cancer Mice

Abstract Views: 303  |  PDF Views: 150

Authors

Eni Widayati
Department of Chemistry, Sultan Agung Islamic University, Jawa Tengah - 50112, Indonesia
Chodidjah
Department of Anatomy, Sultan Agung Islamic University, Jawa Tengah - 50112, Indonesia
Taufiqurrachman Nasihun
Department of Biochemistry, Sultan Agung Islamic University, Semarang, Jawa Tengah - 50112, Indonesia

Abstract


Introduction: Incidence of breast cancer is high and constitutes a significant cause of female mortality. One of breast cancer type is Estrogen and Progesteron Receptor (ER and PR) positive, therefore affected by estrogen and progesteron concentration. Treatment of Thyponium flageliforme in combination with Curcuma zedoaria, and Phyllanthus niruri (TCP) have been proven capable of inhibiting tumor cells proliferation. Objective: To evaluate the expression of protein estrogen receptor and caspase-9 in C3H mice were inoculated with adenocarcinoma cells. Methods: 18 mice were inoculated with one million cells of breast adenocarcinoma per mouse and assigned into three groups. TCP with dosage 85 and 125 mg were treated for 21 days to evaluate the expression of protein estrogen receptor and caspase-9 measured by immuno-histochemical staining. Results: Mann Whitney analysis demonstrated that expression of protein estrogen receptor and caspase-9 in TCP 125 was significant (p < 0.05). Conclusion: Expression of protein estrogen receptor was decreased and caspase-9 expression was increased by TCP administration.

Keywords


Breast Cancer, Caspase9, Combination of Herbal Extracts, Estrogen Receptors.

References





DOI: https://doi.org/10.18311/jnr%2F2018%2F8539