Open Access Open Access  Restricted Access Subscription Access

Identification and Quantification of Boeravinone-B in Dry Fruit Extract of Boerhaavia diffusa Linn and in its Polyherbal Formulation


Affiliations
1 Central Council for Research in Ayurvedic Sciences, Janakpuri - 110058, New Delhi, India
 

Objective: To ascertain the authenticity of Boeravinone-B in Boerhaavia diffusa and its poly-herbal dosage form (capsule) by identification and quantification through precise and robust HPLC method. Materials and Methods: The RP-HPLC analysis was accomplished employing phosphate buffer & acetonitrile as mobile phase at a flow rate of 1.5 ml/min and 270 nm as detection wavelength. Results: The content of Boeravinone B in ingredients (hydro-alcoholic extracts of whole plant) and poly-herbal formulation of B. diffusa was estimated as 0.041 % w/w and 0.011 % w/w, respectively with good linearity R2> 0.9997 and retention time 24.413± 0.004 min. Conclusion: The amount of Boeravinone- B present in B.diffusa extracts of raw drug complies with that of reported in literature. Quantification of Boeravinone-B suggested an identification mark for this drug and its different market formulations and may work as quality parameter in standardization. On the basis of percentage of Boeravinone-B the content of B.diffusa in poly-herbal formulation validate the ratio of ingredient in final formulation. This method proved its applicability in quality assessment via quantification of this marker in raw material as well as various phyto-formulations of B.diffusa and widens the acceptability criteria at international level.

Keywords

Boerhaavia diffusa Formulation Analysis, Boerhaavia diffusa Whole Plant Extract, Boeravinone-B, Widely Distributed in India, Sri Lanka, Ph.
Font Size

User

Notifications
JOURNAL COVERS
  

  • Chauhan A, Semwal DK, Mishra SP, Semwal RB. Ayurvedic research and methodology: Present status and future strategies. AYU. 2015 Oct-Dec; 36(4):364–9. https://doi.org/10.4103/0974-8520.190699 PMid:27833362 PMCid : PMC5041382
  • Lad V. The complete book of ayurvedic home remedies. New York: Three Rivers Press; 1999.
  • Chopra GL. Angiosperms. systematic and life cycle. Jalandhar, Punjab, India: S. Nagin and Co; 1969. p. 361–5.
  • Mishra S, Aeri V, Gaur PV, Jachak SM. Phytochemical, therapeutic, and ethnopharmacological overview for a traditionally important herb: Boerhavia diffusa Linn. Hindawi Publishing Corporation BioMed Research International; 2014. Article ID 808302:1-19.
  • Wahi AK, Agrawal VK, Gupta RC. Phytochemical and pharmacological studies on Boerhaavia diffusa Linn (punarnava) alkaloids. National Academy of Science Letters. 1997; 20.
  • Kirtikar KR, Basu BD. Boerhaavia diffusa. Indian Medicinal Plants. Allahabad, India: Lalit Mohan Basu Publications; 1956. p. 2045–8.
  • Chakraborti KK, Handa SS. Antihepatotoxic activity of Boerhaavia diffusa. Indian Drugs. 1989; 27(13):161–6.
  • Aslam M. Asian medicine and its practice in Britain. In: Evans WC, editor. Pharmacognosy. London UK: Saunders Company Ltd; 1996. p. 499–500.
  • Rawat AK, Mehrotra S, Tripathi SC, Shome U. Hepatoprotective activity of Boerhaavia diffusa L.ischolar_mains-a popular Indian ethnomedicin. Journal of Ethnopharmacology. 1997; 56(1):61–6. https://doi.org/10.1016/S0378-8741(96)01507-3
  • Garg S. Proteins and free amino acid composition of certain Indian desert plants. I. Transactions of the Indian Society Desert Technology, University of Desert Studies.1978; 3(1):31–4.
  • Subramani P, Gan ST, Sokkalingam AD. Polyherbal formulation: Concept of ayurveda. Pharmacognosy Review. 2014; 8(16):73–80. https://doi.org/10.4103/09737847.134229 PMid:25125878 PMCid:PMC4127824
  • Lami N, Kadota S, Kikuchi T, Momose Y. Constituents of the ischolar_mains of Boerhaavia diffusa L. III. Identification of a Ca channel antagonistic compound from the methanol extract. Chemical and Pharmacological Bulletin. 1991; 39(6):1551–5. https://doi.org/10.1248/cpb.39.1551
  • Liogier A. Plantas medicinales de puerto rico ydel caribe.Iberoamerican de Ediciones Inc. San Juan. 1990; 123–7.
  • Szepesi GJ. Planar Chromatogr. Mod TLC. 1993; 6:187.
  • USP, Validation of Compendial Methods 1225. The Pharmacopoeia of the United States of America, Rockville, MD: United States Pharmacopoeial Convention Inc; 2002. p. 2256–9.
  • Agrawal B, Das S, Pandey A. Boerhaavia diffusa Linn.: A review on its phytochemical and pharmacological profile. Asian Journal of Applied Sciences. 2011; 4(7):663–84. https://doi.org/10.3923/ajaps.2011.663.684
  • Indian Pharmacopoeia. Indian Pharmacopoeia Committee. New Delhi: Ministry of Health and Family Welfare, Government of India; 2007. p. 1423–4.
  • Ayurvedic Pharmacopoeia of India. Dept of AYUSH. New Delhi: Ministry of Health and Family Welfare, Government of India; 1990. p. 126–8.
  • Miralles J, Noba K, Ba AT, Gaydou EM, Kornprobst JM. Chemotaxonomy in Nyctagynaceae family: sterols and fatty acids from the leaves of three Boerhaavia species.Biochemical Systematics and Ecology. 1988; 16(5):475–8.https://doi.org/10.1016/0305-1978(88)90047-6
  • Bairwa K, Srivastava A, Jachak SM. Quantitative analysis of Boeravinone-B in the ischolar_mains of Boerhaavia diffusa by UPLC/PDA. Phytochemical Analysis. 2014. https://doi.org/10.1002/pca.2509 PMid:24677242
  • Christian M. Flavonoids-chemical constituents of Boerhaavia diffusa l. through TLC and HPTLC. International Research Journal of Chemistry. 27–36.
  • Nayak P, Thirunavoukkarasu M. A review of the plant Boerhaavia diffusa: Its chemistry, pharmacology and therapeutical potential. The Journal of Phytopharmacology. 2016; 5(2):83–92.
  • Aslam M. Asian medicine and its practice in Britain. In: Evans WC, editor. Pharmacognosy. Saunders Company Ltd, London UK.1996; 499–500.
  • Gupta J, Ali M. Chemical constituents of Boerhaavia diffusa Linn. Roots. Ind J Chem. 1998; 37:912–7.
  • Kadota S, Lami N, Tezuka Y, Kikuchi T. Constituents of the ischolar_mains of Boerhaavia diffusa Linn. I. Examination of sterols and structures of new rotenoids (boeravinones A and B).Chemical Pharmaceutical Bullet.1989; 37(12):3214–20. https://doi.org/10.1248/cpb.37.3214
  • Lami N, Kadota S, Tezuka Y, Kikuchi T. Constituents of the ischolar_mains of Boerhaavia diffusa Linn. II. Structure and stereochemistry of a new rotenoid boeravinone C2. Chemical Pharmaceutical Bullet.1990; 38(6):1558–62. https://doi.org/10.1248/cpb.38.1558
  • Lami N, Kadota S, Kikuchi T, Momose Y. Constituents of the ischolar_mains of Boerhaavia diffusa L. III. Identification of a Ca+2 channel antagonistic compound from the methanol extract. Chemical Pharmaceutical Bullet. 1991; 39(6):1551–5. https://doi.org/10.1248/cpb.39.1551
  • Shukla A, Gupta RK. Effect of the aqueous extract of Boerhavia Difusa ischolar_mains and leaves of hypoglycemic as well as immuno support activity. International Research Journal of Biological Sciences. 2012; 1(6):62–5.
  • Aviello G, Canadanovic-Brunet JM, Milic N, Capasso R, Fattorusso E, Taglialatela-Scafati O. Potent antioxidant and genoprotective effects of Boeravinone G, a rotenoid isolated from Boerhaavia diffusa. PLoS ONE.2011; 6(5).https://doi.org/10.1371/journal.pone.0019628 PMid:21625488 PMCid:PMC3098844
  • Lal VKP, Verma A. HPTLC densitogram profile of aqueous distillate (Arka) from ischolar_mains of Boerhaavia diffusa. BJPR. 2016; 9(1):1–6. https://doi.org/10.9734/BJPR/2016/19399
  • Vijayakrishna AC, Shreedhra CS, Latha KP, Belvotagi AV. Thin layer chromatography as a tool for quality control of punarnava. J of Pharmaceutical and Scientific Innovation.2014 Jul-Aug; 3(4):375–8. https://doi.org/10.7897/22774572.034177
  • Baralcat HH, Sahar Hussin AM, Mohamed Marzouk S, Merfort I, Linscheid M, Nawwar Mohamed AM. Polyphenolic metabolities of Epilobium hirsutisam.Phytochemistry. 1997; 46:935–41. https://doi.org/10.1016/ S0031-9422(97)00370-1
  • Bairwa K, Srivastava A, Jachak SM. Phytochem Anal. 2014 Sep-Oct; 25(5):415–20. https://doi.org/10.1002/pca.2509 PMid:24677242
  • Aldon G, Vikas VV, Ravikiran DB, Jaydeep G. Seapration and questification of pharmacologically active markers boeravinone B, eupalitin-3-o-β-sitosterol from Boerhavia diffusa Linn and from marketed formulations. International Journal of Pharmacy and Pharmaceutical Sciences. 2013; 5(3).
  • Vaidya VV, Gomes A, Gadgil J, Patil S. HPLC analysis of Boeravinone B and eupalitin-3-o- β-d-galactopyranoside from plant and formulation of Boerhavia diffusa linn. IJRPC. 2014; 4(4):982–6.
  • Quality Standards of Indian Medicinal Plants. New Delhi: Indian Council of Medical Research; 2011. p. 59–71.

Abstract Views: 275

PDF Views: 153




  • Identification and Quantification of Boeravinone-B in Dry Fruit Extract of Boerhaavia diffusa Linn and in its Polyherbal Formulation

Abstract Views: 275  |  PDF Views: 153

Authors

A. Singh
Central Council for Research in Ayurvedic Sciences, Janakpuri - 110058, New Delhi, India
H. Sharma
Central Council for Research in Ayurvedic Sciences, Janakpuri - 110058, New Delhi, India
R. Singh
Central Council for Research in Ayurvedic Sciences, Janakpuri - 110058, New Delhi, India
P. Pant
Central Council for Research in Ayurvedic Sciences, Janakpuri - 110058, New Delhi, India
N. Srikant
Central Council for Research in Ayurvedic Sciences, Janakpuri - 110058, New Delhi, India
K. S. Dhiman
Central Council for Research in Ayurvedic Sciences, Janakpuri - 110058, New Delhi, India

Abstract


Objective: To ascertain the authenticity of Boeravinone-B in Boerhaavia diffusa and its poly-herbal dosage form (capsule) by identification and quantification through precise and robust HPLC method. Materials and Methods: The RP-HPLC analysis was accomplished employing phosphate buffer & acetonitrile as mobile phase at a flow rate of 1.5 ml/min and 270 nm as detection wavelength. Results: The content of Boeravinone B in ingredients (hydro-alcoholic extracts of whole plant) and poly-herbal formulation of B. diffusa was estimated as 0.041 % w/w and 0.011 % w/w, respectively with good linearity R2> 0.9997 and retention time 24.413± 0.004 min. Conclusion: The amount of Boeravinone- B present in B.diffusa extracts of raw drug complies with that of reported in literature. Quantification of Boeravinone-B suggested an identification mark for this drug and its different market formulations and may work as quality parameter in standardization. On the basis of percentage of Boeravinone-B the content of B.diffusa in poly-herbal formulation validate the ratio of ingredient in final formulation. This method proved its applicability in quality assessment via quantification of this marker in raw material as well as various phyto-formulations of B.diffusa and widens the acceptability criteria at international level.

Keywords


Boerhaavia diffusa Formulation Analysis, Boerhaavia diffusa Whole Plant Extract, Boeravinone-B, Widely Distributed in India, Sri Lanka, Ph.

References