Journal of Natural Remedies

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Isolation and Characterization of Bioactive Moiety from Leaf Extract of Ipomoea mauritiana and Evaluation of Anti-Inflammatory Activity by Carrageenan Induced Rat Paw Edema Test


Affiliations
1 Department of Microbiology, Yuvaraja’s College, University of Mysore, Mysuru - 570005, Karnataka, India
2 Department of Studies in Microbiology, Manasa Gangothri, University of Mysore, Mysuru - 570006, Karnataka, India
3 Drosophila Stock Centre, Department of Studies in Zoology, Manasa Gangothri, University of Mysore, Mysuru - 570006, Karnataka, India
 

Ipomoea mauritiana Jacq. is a member of family Convolvulaceae and commonly called as Ksheervidari is widely used in various traditional medicines for the treatment of many diseases. In the present work the column fractions of leaf extracts of I. mauritiana obtained by Soxhlet exrtaction was subjected to TLC, column chromatography, HPLC and liquid chromatography and mass spectroscopy (LC-MS). The compounds like caffeic acid and β-amyric acetate were tentatively identified to be present in one of the purified fraction. The column fraction was also tested for anti-inflammatory activity by carrageenan induced rat paw edema assay. The column fraction tested showed a very good anti inflammatory activity.

Keywords

Anti Inflammatory Activity, Carrageenan Induced Rat Paw Edema Column, Chromatography, Ipomoea mauritiana.
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  • Sharma AK, Dutta PC. Cytological investigations on the genus Ipomoea and its importance in the study of phylogeny. The Nucleus 1958; 1:89–122.

  • Meira M, da Silva EP, David JM, David JP. Review of the genus Ipomoea: Traditional uses, chemistry and biological activities. Braz J Pharmacog. 2012; 22(3):682–713. https:// doi.org/10.1590/S0102-695X2012005000025

  • Li JWH, Vederas JC. Drug discovery and natural products: End of an era or an endless frontier? Science. 2009; 325:161–5. https://doi.org/10.1126/science.1168243 PMid:19589993

  • Pereda-Miranda R, Bah M. Biodynamic constituents in the mexican morning glories: Purgative remedies transcending boundaries. Curr Top Med Chem. 2003; 3:111–31. https://doi.org/10.2174/1568026033392534 PMid:12570768

  • Chopra RN, Nayar SL, Chopra IC. Glossary of Indian medicinal plants. New Delhi, India: Council for Scientific and Industrial Research, Government of India; 1992.

  • Pandey G. Dravyaguna Vijnana, Part III Reprint, 2004. Chowkhamba Krishnadas Academy, Varanasi, India.

  • Vijender K, Zulfiqar AB, Dinesh K, Khan NA, Chashoo IA: Evaluation of anti inflammatory potential of leaf extracts of Skimmiaan quetilia. Asian Pac J Trop Biomed. 2012; 2(8):627–30. https://doi.org/10.1016/S22211691(12)60109-9

  • Khan MS, Nitin MDN, Kharya, Khanam S. Chromatographic estimation of maturity based phytochemical profiling of Ipomoea mauritiana. Int J Phytomed. 2009; 1:22–30.https://doi.org/10.5138/ijpm.2009.0975.0185.05790

  • The Wealth of India, Raw Materials. New Delhi, India: Council of Scientific and Industrial Research; 1992. p.249–51.

  • Kritikar KR, Basu BD. Indian Medicinal Plants (Illustrated).3rd ed. Delhi, India: Sri Satguru Publication; 2000. p.2368–3269.


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PDF Views: 171




  • Isolation and Characterization of Bioactive Moiety from Leaf Extract of Ipomoea mauritiana and Evaluation of Anti-Inflammatory Activity by Carrageenan Induced Rat Paw Edema Test

Abstract Views: 277  |  PDF Views: 171

Authors

H. V. Pavan
Department of Microbiology, Yuvaraja’s College, University of Mysore, Mysuru - 570005, Karnataka, India
S. Mahadeva Murthy
Department of Microbiology, Yuvaraja’s College, University of Mysore, Mysuru - 570005, Karnataka, India
N. Lakshmi Devi
Department of Studies in Microbiology, Manasa Gangothri, University of Mysore, Mysuru - 570006, Karnataka, India
M. K. Ramakrishna
Drosophila Stock Centre, Department of Studies in Zoology, Manasa Gangothri, University of Mysore, Mysuru - 570006, Karnataka, India

Abstract


Ipomoea mauritiana Jacq. is a member of family Convolvulaceae and commonly called as Ksheervidari is widely used in various traditional medicines for the treatment of many diseases. In the present work the column fractions of leaf extracts of I. mauritiana obtained by Soxhlet exrtaction was subjected to TLC, column chromatography, HPLC and liquid chromatography and mass spectroscopy (LC-MS). The compounds like caffeic acid and β-amyric acetate were tentatively identified to be present in one of the purified fraction. The column fraction was also tested for anti-inflammatory activity by carrageenan induced rat paw edema assay. The column fraction tested showed a very good anti inflammatory activity.

Keywords


Anti Inflammatory Activity, Carrageenan Induced Rat Paw Edema Column, Chromatography, Ipomoea mauritiana.

References