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Antioxidant and Anti Aging Assays of Oryza sativa Extracts, Vanillin and Coumaric Acid


Affiliations
1 Medical Research Center, Maranatha Christian University, Jl Prof Drg Surya Sumantri No 65 Bandung 40164, West Java, Indonesia
2 Aretha Medika Utama, Biomolecular and Biomedical Research Center, Jl Babakan Jeruk 2, No 9, Bandung 40163, West Java, Indonesia
 

Aging is a natural process in humans as accumulation of oxygen-derived free radicals which leads to the activation of hyaluronidase, collagenase and elastase, that can further contribute to cellular and tissue damage. Bioactive compounds from plants have been used as antioxidant that might inhibit aging processes as well. This study aimed to determine antioxidant and anti aging properties of Oryza sativa Extract (OSE), and its compounds, vanillin and coumaric acid. The phytochemical analysis of OSE was performed with Farnsworth modified method. Antioxidant activities were performed by measurement of 2,2-diphenyl 1-pichylhydazyl (DPPH) free radical scavenger, Ferric Reducing Antioxidant Power (FRAP), and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) reducing activity, while anti aging assay were observed through inhibitory of elastase, collagenase, and hyaluronidase activities. Phytochemical analysis showed the presence of terpenoids and saponins in high level. OSE showed lowest DPPH activity (IC50 = 314.51 μg/mL) compared to vanillin (IC50 = 283 μg/mL) and coumaric acid (IC50 = 255.69 μg/mL). In ABTS assay, OSE resulted lowest activity(IC50 = 145.67 μg/mL), compared to vanillin (IC50 = 4.96 μg/mL) and coumaric acid (IC50 = 1.67 μg/mL). OSE also showed the lowest FRAP-reducing activity (21.26 μM Fe(II)/μg), compared to vanillin (35.05 μM Fe(II)/μg) and coumaric acid (48.52μM Fe(II)/μg). OSE showed the lowest collagenase, elastase, and hyaluronidase inhibitory activity (IC50 = 816.78,107.51, and 203.13 μg/mL), compared to vanillin (IC50 = 16.27, 14.46, 45.23 μg/mL respectively) and coumaric acid (IC50 = 146.89, 25.38, 8.21 μg/mL respectively). In summary, OSE possess the lowest antioxidant and anti aging activities compared to vanillin and coumaric acid.

Keywords

Antioxidant, Anti Aging, Coumaric Acid, Oryza sativa, Vanillin.
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  • Antioxidant and Anti Aging Assays of Oryza sativa Extracts, Vanillin and Coumaric Acid

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Authors

Wahyu Widowati
Medical Research Center, Maranatha Christian University, Jl Prof Drg Surya Sumantri No 65 Bandung 40164, West Java, Indonesia
Nurul Fauziah
Medical Research Center, Maranatha Christian University, Jl Prof Drg Surya Sumantri No 65 Bandung 40164, West Java, Indonesia
Heddy Herdiman
Medical Research Center, Maranatha Christian University, Jl Prof Drg Surya Sumantri No 65 Bandung 40164, West Java, Indonesia
Merry Afni
Aretha Medika Utama, Biomolecular and Biomedical Research Center, Jl Babakan Jeruk 2, No 9, Bandung 40163, West Java, Indonesia
Ervi Afifah
Aretha Medika Utama, Biomolecular and Biomedical Research Center, Jl Babakan Jeruk 2, No 9, Bandung 40163, West Java, Indonesia
Hanna Sari W. Kusuma
Aretha Medika Utama, Biomolecular and Biomedical Research Center, Jl Babakan Jeruk 2, No 9, Bandung 40163, West Java, Indonesia
Hayatun Nufus
Aretha Medika Utama, Biomolecular and Biomedical Research Center, Jl Babakan Jeruk 2, No 9, Bandung 40163, West Java, Indonesia
Seila Arumwardana
Aretha Medika Utama, Biomolecular and Biomedical Research Center, Jl Babakan Jeruk 2, No 9, Bandung 40163, West Java, Indonesia
Dwi Davidson Rihibiha
Aretha Medika Utama, Biomolecular and Biomedical Research Center, Jl Babakan Jeruk 2, No 9, Bandung 40163, West Java, Indonesia

Abstract


Aging is a natural process in humans as accumulation of oxygen-derived free radicals which leads to the activation of hyaluronidase, collagenase and elastase, that can further contribute to cellular and tissue damage. Bioactive compounds from plants have been used as antioxidant that might inhibit aging processes as well. This study aimed to determine antioxidant and anti aging properties of Oryza sativa Extract (OSE), and its compounds, vanillin and coumaric acid. The phytochemical analysis of OSE was performed with Farnsworth modified method. Antioxidant activities were performed by measurement of 2,2-diphenyl 1-pichylhydazyl (DPPH) free radical scavenger, Ferric Reducing Antioxidant Power (FRAP), and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) reducing activity, while anti aging assay were observed through inhibitory of elastase, collagenase, and hyaluronidase activities. Phytochemical analysis showed the presence of terpenoids and saponins in high level. OSE showed lowest DPPH activity (IC50 = 314.51 μg/mL) compared to vanillin (IC50 = 283 μg/mL) and coumaric acid (IC50 = 255.69 μg/mL). In ABTS assay, OSE resulted lowest activity(IC50 = 145.67 μg/mL), compared to vanillin (IC50 = 4.96 μg/mL) and coumaric acid (IC50 = 1.67 μg/mL). OSE also showed the lowest FRAP-reducing activity (21.26 μM Fe(II)/μg), compared to vanillin (35.05 μM Fe(II)/μg) and coumaric acid (48.52μM Fe(II)/μg). OSE showed the lowest collagenase, elastase, and hyaluronidase inhibitory activity (IC50 = 816.78,107.51, and 203.13 μg/mL), compared to vanillin (IC50 = 16.27, 14.46, 45.23 μg/mL respectively) and coumaric acid (IC50 = 146.89, 25.38, 8.21 μg/mL respectively). In summary, OSE possess the lowest antioxidant and anti aging activities compared to vanillin and coumaric acid.

Keywords


Antioxidant, Anti Aging, Coumaric Acid, Oryza sativa, Vanillin.

References





DOI: https://doi.org/10.18311/jnr%2F2016%2F7220