Journal of Biological Control

Open Access Open Access  Restricted Access Subscription Access

Bioefficacy and mass culturing of Heterorhabditis bacteriophora (Nematoda:Heterorhabditidae) on cabbage butterfly, Pieris brassicae (Lepidoptera:Pieridae) in temperate region of Kashmir, Indi


Affiliations
1 Division of Entomology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar Campus, Srinagar – 190 025, Jammu and Kashmir, India
2 Department of Zoology, University of Kashmir, Srinagar – 190 006, Jammu and Kashmir, India
 

A laboratory investigation was carried out to assess the bioefficacy and mass culturing of Kashmir isolate of entomopathogenic nematode (EPN), Heterorhabditis bacteriophora on cabbage butterfly, Pieris brassicae in Srinagar. The pathogenicity of H. bacteriophora against 3rd, 4th and 5th larval instars of P. brassicae showed cent per cent mortality of the pest in all the three larval instars at the dosages of 20 IJs/larva and above. The LC50 values of H. bacteriophora against 3rd, 4th and 5th larval instars of P. brassicae ranged from 46.61 to 9.32, 76.33 to 9.82 and 91.36 to 10.08 IJs/larva at 24 to 96 h and that the LT50 values from 65.15 to 23.12, 84.54 to 29.20 and 105.36 to 33.52 h at 10 to 60 IJs/larva, respectively. The average production of nematode infective juveniles per larvae was found to be 7.02 x 103, 25.74 x 103 and 58.45 x 103 and that from per gram of host body weight was 91.49 x 103, 151.30 x 103 and 233.78 x 103 in 3rd, 4th and 5th larval instars of P. brassicae, respectively. The total time period between the larval mortality and the initiation of emergence of the infective juveniles from the cadavers ranged from 7-8, 9-10 and 10-11 days and the peak period of emergence of infective juveniles ranged from 10-13, 14-19, 16-21 days after mortality from the cadavers of 3rd, 4th and 5th larval instars of P. brassicae, respectively. The total duration of nematode emergence recorded during the period between larval mortality and cessation of emergence of infective juveniles from the cadavers ranged 17-19, 23-25 and 26-29 days after mortality in case of 3rd, 4th and 5th larval instars of P. brassicae, respectively.

Keywords

Bioefficacy, Entomopathogenic Nematode, Heterorhabditis bacteriophora, Pieris brassicae
User
Notifications

  • Divya K, Sankar M. 2009. Entomopathogenic nematodes in pest management. Indian J Sci Technol. 2:53–60. https://doi.org/10.17485/ijst/2009/v2i7.12

  • Flanders KL, Miller JM, Shields EJ. 1996. In vivo production of Heterorhabditis bacteriophora 'Oswego' (Rhabditida: Heterorhabditidae), a potential biological control agent for soil-inhabiting insects in temperate regions. J Econ Entom. 89:373–380. https://doi.org/10.1093/jee/89.2.373

  • Karunakar G, Easwaramoorthy S, David H. 2000. Host parasite interaction between two species of white grubs infesting sugarcane and two species of entomopathogenic nematodes. Sug Technol. 2(4):12–16. https://doi.org/10.1007/BF02995579

  • Kaya HK. 1985. Entomogenous nematodes for insect control in IPM systems. pp. 283–302. In: Hoy MA. and Herzog DC. (Eds.). Biological Control in Agriculture IPM Systems. New York: Academic Press. https://doi.org/10.1016/B978-0-12-357030-7.50022-3

  • Kaya HK. 1990. Soil Ecology. Entomopathogenic nematodes. Ann Rev Entomol. 38:181–206. https://doi.org/10.1146/annurev.en.38.010193.001145

  • Khan MR, Khan U, Askary TH, Mohiddin FA, Khan MM. 2007. Pathogenicity and host suitability for in vivo mass production of Steinernema masoodi AMU EPN-1. Int J Nematol. 17:151–157.

  • Rajkumar M, Parihar A, Siddiqui AU. 2002. Mass multiplication of indigenous entomopathogenic nematodes of Udaipur. Proceedings of National Symposium on biodiversity and management of nematodes in cropping systems for sustainable agriculture, November 11-13, 2002. ARS, Durgapura, Jaipur, Rajasthan, India; p. 77.


Abstract Views: 236

PDF Views: 235




  • Bioefficacy and mass culturing of Heterorhabditis bacteriophora (Nematoda:Heterorhabditidae) on cabbage butterfly, Pieris brassicae (Lepidoptera:Pieridae) in temperate region of Kashmir, Indi

Abstract Views: 236  |  PDF Views: 235

Authors

M. A. Mantoo
Division of Entomology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar Campus, Srinagar – 190 025, Jammu and Kashmir, India
F. A. Zaki
Division of Entomology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar Campus, Srinagar – 190 025, Jammu and Kashmir, India
Rasi Jan
Department of Zoology, University of Kashmir, Srinagar – 190 006, Jammu and Kashmir, India

Abstract


A laboratory investigation was carried out to assess the bioefficacy and mass culturing of Kashmir isolate of entomopathogenic nematode (EPN), Heterorhabditis bacteriophora on cabbage butterfly, Pieris brassicae in Srinagar. The pathogenicity of H. bacteriophora against 3rd, 4th and 5th larval instars of P. brassicae showed cent per cent mortality of the pest in all the three larval instars at the dosages of 20 IJs/larva and above. The LC50 values of H. bacteriophora against 3rd, 4th and 5th larval instars of P. brassicae ranged from 46.61 to 9.32, 76.33 to 9.82 and 91.36 to 10.08 IJs/larva at 24 to 96 h and that the LT50 values from 65.15 to 23.12, 84.54 to 29.20 and 105.36 to 33.52 h at 10 to 60 IJs/larva, respectively. The average production of nematode infective juveniles per larvae was found to be 7.02 x 103, 25.74 x 103 and 58.45 x 103 and that from per gram of host body weight was 91.49 x 103, 151.30 x 103 and 233.78 x 103 in 3rd, 4th and 5th larval instars of P. brassicae, respectively. The total time period between the larval mortality and the initiation of emergence of the infective juveniles from the cadavers ranged from 7-8, 9-10 and 10-11 days and the peak period of emergence of infective juveniles ranged from 10-13, 14-19, 16-21 days after mortality from the cadavers of 3rd, 4th and 5th larval instars of P. brassicae, respectively. The total duration of nematode emergence recorded during the period between larval mortality and cessation of emergence of infective juveniles from the cadavers ranged 17-19, 23-25 and 26-29 days after mortality in case of 3rd, 4th and 5th larval instars of P. brassicae, respectively.

Keywords


Bioefficacy, Entomopathogenic Nematode, Heterorhabditis bacteriophora, Pieris brassicae

References





DOI: https://doi.org/10.18311/jbc%2F2021%2F23101