Journal of Biological Control

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Deciphering the network of interconnected pathways of Chaetomium globosum antagonistic related genes against Bipolaris sorokiniana using RNA seq approach


Affiliations
1 Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR - Indian Agricultural Research Institute, New Delhi – 110 012, India
 

Chaetomium species are known as potential biocontrol agents against phytopathogens due to their multiple antagonistic mechanisms. Plant disease is controlled by Chaetomium exhibit complex interactions against plant pathogen under varied conditions. Previously, mycoparasitism and antibiosis have been reported as most effective mechanism exhibited by the C. globosum against Bipolaris sorokiniana. In the present study, the examination of major biosynthetic pathways underlying Chaetomium globosum biocontrol activity was elucidated. It was shown that the pathways related to biosynthesis of secondary metabolites, hydrolytic enzymes and other key regulator genes were involved in production of hydrolytic enzymes and antifungal metabolites. We identified various genes of biological function with significant log2 fold change such as phosphoribosyl aminoimidazole carboxylase (9.693), protease (8.18), cyanate hydratase (Cyanase) (6.7), Fe2OG dioxygenase domain-containing protein (5.9), superoxide dismutase (5.55), glycosidase (5.34), carboxylic ester hydrolase (5.27), alpha-1,2-Mannosidase (4.44), alpha-1,4 glucan phosphorylase (3.99), endochitinase (3.87), P53-like transcription factor (Fragment) (3.55), metalloprotease (3.4), polyketide synthase (3.35), Catalase-peroxidase (CP) (3.14), protein kinase domain-containing protein (3.18) and glutamate decarboxylase (2.1) which are involved in biosynthesis of secondary metabolites, polyketide synthase, antibiotic, hydrolytic enzymes and putative fungistatic metabolites. This data provides a good foundation for continued researches into C. globosum Cg2 biocontrol activity for facilitating widespread application under the field conditions.


Keywords

Bio-control, Bipolaris sorokiniana, Chaetomium globosum, CNV, interconnected pathways, secondary metabolites
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  • Deciphering the network of interconnected pathways of Chaetomium globosum antagonistic related genes against Bipolaris sorokiniana using RNA seq approach

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Authors

Darshan K.
Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR - Indian Agricultural Research Institute, New Delhi – 110 012, India
Rashmi Aggarwal
Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR - Indian Agricultural Research Institute, New Delhi – 110 012, India
Bishnu Maya Bashyal
Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR - Indian Agricultural Research Institute, New Delhi – 110 012, India
M. Haritha Mohan
Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR - Indian Agricultural Research Institute, New Delhi – 110 012, India

Abstract


Chaetomium species are known as potential biocontrol agents against phytopathogens due to their multiple antagonistic mechanisms. Plant disease is controlled by Chaetomium exhibit complex interactions against plant pathogen under varied conditions. Previously, mycoparasitism and antibiosis have been reported as most effective mechanism exhibited by the C. globosum against Bipolaris sorokiniana. In the present study, the examination of major biosynthetic pathways underlying Chaetomium globosum biocontrol activity was elucidated. It was shown that the pathways related to biosynthesis of secondary metabolites, hydrolytic enzymes and other key regulator genes were involved in production of hydrolytic enzymes and antifungal metabolites. We identified various genes of biological function with significant log2 fold change such as phosphoribosyl aminoimidazole carboxylase (9.693), protease (8.18), cyanate hydratase (Cyanase) (6.7), Fe2OG dioxygenase domain-containing protein (5.9), superoxide dismutase (5.55), glycosidase (5.34), carboxylic ester hydrolase (5.27), alpha-1,2-Mannosidase (4.44), alpha-1,4 glucan phosphorylase (3.99), endochitinase (3.87), P53-like transcription factor (Fragment) (3.55), metalloprotease (3.4), polyketide synthase (3.35), Catalase-peroxidase (CP) (3.14), protein kinase domain-containing protein (3.18) and glutamate decarboxylase (2.1) which are involved in biosynthesis of secondary metabolites, polyketide synthase, antibiotic, hydrolytic enzymes and putative fungistatic metabolites. This data provides a good foundation for continued researches into C. globosum Cg2 biocontrol activity for facilitating widespread application under the field conditions.


Keywords


Bio-control, Bipolaris sorokiniana, Chaetomium globosum, CNV, interconnected pathways, secondary metabolites

References





DOI: https://doi.org/10.18311/jbc%2F2020%2F26736