Journal of Biological Control

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Biointensive management of wilt disease of lentil through biocontrol agents and organic amendments


Affiliations
1 Plant Pathology Department, College of Agriculture, Nagaur (AU), Jodhpur – 341001, Rajasthan, India
2 Plant Pathology Department, Rajasthan Agricultural Research Institute, Durgapura, Jaipur – 302018, Rajasthan, India
 

Lentil (Lens culinaris M.) is an important dietary source of protein and other essential nutrients in South and West Asia, North and East Africa. Lentil crops are susceptible to a number of diseases caused by fungi and other parasites. Among them, the most significant and serious soil-borne disease is Fusarium wilt (Fusarium oxysporum f.sp. lentis: Fol). Systematic studies in laboratory, cage house and field trials were conducted (2016-17 to 2017-18). Laboratory experiments were carried out in completely randomized design with four replications and field trials were carried out consecutively during 2016-17 and 2017-2018 crop season in Randomized Block Design (RBD) with four replications using L9-12, a susceptible cultivar. Under in vitro, bio efficacy of selected bio agents, maximum 81.33 per cent mycelial growth inhibition of pathogen was recorded in Trichoderma harzianum (local isolate) and minimum mycelial growth inhibition 55.00 per cent was recorded in Bacillus subtilis. Under in vivo condition, effect of selected bio agents and organic amendments on disease incidence, percent disease control, pathogen spore in per gram soil and yield ha-1 of lentil was recorded. Application of Trichoderma harzianum (Local isolate) @ 6g/Kg seed was found most effective with maximum seed germination 91.95 and 90.62 per cent, lowest disease incidence of 24.22 and 25.39 per cent and higher pooled mean grain yield 774.31 kg/ha over check during 2016-17 and 2017-18, respectively. Among organic amendments, maximum disease control (42.02 per cent), minimum average disease incidence (36.33 per cent) and minimum pathogen spores (1.3x104 per gram soil) were observed in neem cake amendment followed by poultry manure and vermicompost amendments. This indicates that these treatments can be effectively used for controlling Fusarium wilt disease under organic mode of lentil cultivation in Rajasthan.


Keywords

Biocontrol agents, Fusarium oxysporum f.sp. lentis, lentil wilt, organic amendments
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  • Arora DK, Dwivedi RS. 1980. Mycoparasitism of Fusarium spp. on Rhizoctonia solani Kühn. Plant and Soil. 55: 43–53. https://doi.org/10.1007/BF02149707

  • Biederbeck VO, Zentner RP, Campbell CA. 2005. Soil microbial populations and activities as influenced by legume green fallowing in a semiarid loam. Soil Bio and Biochemistry 37: 1775–1784.

  • https://doi.org/10.1016/j.soilbio.2005.02.011

  • Choudhary S, Mohanka R. 2012. In vitro antagonism of indigenous trichoderma isolates against phytopathogen causing wilt of lentil. Int J of Life Sci and Pharma Res. 2: 195-202.

  • Garkoti A, Kumar S, Lal M, Singh V. 2013. Major diseases of lentil: Epidemiology and disease management-A review. Agriways 1: 62–64.

  • Garkoti A, Kumar V, Tripathi HS. 2014. Control of wilt disease of lentil through bio control agents and organic amendments in Tarai region of Uttarakhand, India. J of Envir Bio., 35: 1067–1070.

  • Kumar V, Garkoti A, Tripathi HS. 2013. Management of vascular wilt of lentil through bio control agents and organic amendments in Tarai area of Uttarakhand state. The Bioscan 8: 575–577.

  • Lemke RL, Zhong Z, Campbell CA, Zentner RP. 2007. Can pulse crops play a role in mitigating greenhouse gases from North American agriculture? Agronomy J. 99. https://doi.org/10.2134/agronj2006.0327s

  • Manthan A, Balabaskar P. 2002. In vitro evaluation of fungitoxicity of selected natural products on heavy inoculum and conidial germination of Fusarium moniliforme. Ind Phytopath. 61: 238–242.

  • Mohammadi NE, Goltapeh A, Babaie A, Puralibaba H. 2011. Pathogenic and genetic characterization of Iranian isolates of Fusarium oxysporum f.sp. lentis by ISSR analysis. Int J of Agri Tech. 7: 63–72.

  • Morton DJ, Stroube WH. 1955. Antagonistic and stimulating effects of soil micro-organism of Sclerotium.

  • Phytopathology 45: 417–420.

  • Rajiv K, Dubey DK. 2003. Incidence of Fusarium wilt of lentil (Fusarium oxysporum f.sp. lentis). J Res Birsa Agri Uni. 16: 301–303.

  • Siddique KHM, Johansen C, Turner NC, Jeuffroy MH, Hashem A, Sakar D, Gan Y, Alghamdi SS. 2012.

  • Innovations in agronomy for food legumes A review. Agro Sust Dev. 32. https://doi.org/10.1007/s13593-0110021-5

  • Singh VK, Naresh P, Biswas SK. 2014. Antagonistic evaluation of some bio agents and their effect as seed treatment on germination growth parameters and wilt incidence in lentil. Agriways 2: 126–129.

  • Tharanathan RN, Mahadevamma S. 2003. Grain legumes: A boon to human nutrition. Trends Food Sci Tech. 14: 07–18. https://doi.org/10.1016/j.tifs.2003.07.002

  • Tiwari N, Ahmed S, Kumar S, Sarker A. 2018. Fusarium wilt: A killer disease of lentil. Open access peer-reviewed chapter. https://doi.org/10.5772/intechopen.72508


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  • Biointensive management of wilt disease of lentil through biocontrol agents and organic amendments

Abstract Views: 264  |  PDF Views: 166

Authors

Manju Kumari
Plant Pathology Department, College of Agriculture, Nagaur (AU), Jodhpur – 341001, Rajasthan, India
Kuldeep Sharma
Plant Pathology Department, Rajasthan Agricultural Research Institute, Durgapura, Jaipur – 302018, Rajasthan, India
OM Prakash Sharma
Plant Pathology Department, Rajasthan Agricultural Research Institute, Durgapura, Jaipur – 302018, Rajasthan, India

Abstract


Lentil (Lens culinaris M.) is an important dietary source of protein and other essential nutrients in South and West Asia, North and East Africa. Lentil crops are susceptible to a number of diseases caused by fungi and other parasites. Among them, the most significant and serious soil-borne disease is Fusarium wilt (Fusarium oxysporum f.sp. lentis: Fol). Systematic studies in laboratory, cage house and field trials were conducted (2016-17 to 2017-18). Laboratory experiments were carried out in completely randomized design with four replications and field trials were carried out consecutively during 2016-17 and 2017-2018 crop season in Randomized Block Design (RBD) with four replications using L9-12, a susceptible cultivar. Under in vitro, bio efficacy of selected bio agents, maximum 81.33 per cent mycelial growth inhibition of pathogen was recorded in Trichoderma harzianum (local isolate) and minimum mycelial growth inhibition 55.00 per cent was recorded in Bacillus subtilis. Under in vivo condition, effect of selected bio agents and organic amendments on disease incidence, percent disease control, pathogen spore in per gram soil and yield ha-1 of lentil was recorded. Application of Trichoderma harzianum (Local isolate) @ 6g/Kg seed was found most effective with maximum seed germination 91.95 and 90.62 per cent, lowest disease incidence of 24.22 and 25.39 per cent and higher pooled mean grain yield 774.31 kg/ha over check during 2016-17 and 2017-18, respectively. Among organic amendments, maximum disease control (42.02 per cent), minimum average disease incidence (36.33 per cent) and minimum pathogen spores (1.3x104 per gram soil) were observed in neem cake amendment followed by poultry manure and vermicompost amendments. This indicates that these treatments can be effectively used for controlling Fusarium wilt disease under organic mode of lentil cultivation in Rajasthan.


Keywords


Biocontrol agents, Fusarium oxysporum f.sp. lentis, lentil wilt, organic amendments

References





DOI: https://doi.org/10.18311/jbc%2F2020%2F25570