Journal of Biological Control

Open Access Open Access  Restricted Access Subscription Access

Assessment of Nematicidal Properties of Fluorescent Pseudomonads using Peanut Root-Knot Nematode, Meloidogyne arenaria


Affiliations
1 ICAR-Directorate of Groundnut Research, Junagadh - 362001, Gujarat, India
 

The primary objective of this study was to identify natural isolates of fluorescent pseudomonads with superior antagonistic activity towards plant parasitic nematodes. Nematicidal potential of eighteen isolates of fluorescent pseudomonads were compared against peanut ischolar_main-knot nematode, Meloidogyne arenaria. Cell-free culture filtrate of DAPG-producing isolates of Pseudomonas putida caused significantly higher mortality of M. arenaria (J2) with highest in isolate DAPG3 (87.36%), followed by DAPG1 (84.16%) compared to other isolates of fluorescent pseudomonads, i.e., P. gessardii BHU1 and P. aeruginosa BM6 after exposure period of 72h at 100% concentration. The selected DAPG-producing isolates of P. putida caused significant inhibition in egg hatching. The lowest cumulative per cent hatch of M. arenaria was observed in the isolate DAPG3 (17.84%) followed by DAPG1 (18.10%). The isolates DAPG1 and DAPG3 also inhibited the nematode invasion in the ischolar_mains of peanut by 41.30% and 36.34%, respectively. Significant reduction in number of galls/plant in peanut ischolar_mains was recorded. The maximum reduction (51.30%) in ischolar_main galling was recorded with combination of seed treatment and soil application of P. putida DAPG1 followed by 41.73% in combined treatment of seed treatment and soil application of P. putida DAPG3. The levels of Peroxidase (POD), Catalase (CAT) and Polyphenol Oxidase (PPO) were non-significant in the leaves of peanut in the treatment that received P. putida DAPG1 and DAPG3, either as seed treatment and/or soil application, compared to inoculated and un-inoculated control. However, significantly enhanced phenol content was recorded in the leaves of peanut in the treatment that received combination of seed treatment and soil application of P. putida DAPG1 and seed treatment alone.

Keywords

Fluorescent Pseudomonads, Meloidogyne arenaria, Nematicidal Activity, Peanut.
User
Notifications

  • Aalten PM, Vitour D, Blanvillain D, Gowen SR, Sutra L. 1998. Effect of rhizosphere fluorescent Pseudomonas isolates on plant parasitic nematodes Radopholus similis and Meloidogyne spp. Lett Appl Microbiol. 27: 357–361. https://doi.org/10.1046/j.1472-765X.1998.00440.x

  • Aebi H. 1984. Catalase in vitro. Methods Enzymol. 105: 121– 126. https://doi.org/10.1016/S00766879(84)05016-3

  • Ahmadzadeh M, Afsharmanesh HJ, Nikkhah M, SharifiTehrani A. 2006. Identification of some molecular traits in fluorescent pseudomonads with antifungal activity. Iranian J Biotechnol. 4: 245-253.

  • Becker JO, Zavaleta-Mejia E, Colbert, SF, Schroth MN, Weinhold AR, Hancock GJ, Van Gundy SD. 1988.

  • Effects of rhizobacteria on ischolar_main-knot nematodes and gall formation. Phytopathology 78: 1466–1469. https://doi.org/10.1094/Phyto-78-1466

  • Bhayani S, Shah A, Pal KK, Dey R, Dash P, Chauhan SM, Parmar V, Chudasama A. 2013. DAPG-producing fluorescent pseudomonads for enhancing growth and yield of groundnut. National Conference of Plant Physiology on “Current Trends in Plant Biology Research”, 13-16 December, Junagadh. pp. 818-819.

  • Brazelton JN, Pfeufer EE, Sweat TA, McSpadden Gardener BB, Coenen C. 2008. 2,4-Diacetylphloroglucinol alters plant ischolar_main development. Mol Plant Microbe Interact. 21: 1349–1358.

  • https://doi.org/10.1094/MPMI-21-10-1349 PMid:18785830

  • Byrd DW, Kirkpatrick Jr. T, Barker KR. 1983. An improved technique for clearing and staining plant tissue for detection of nematodes. J Nematol. 14: 142–143.

  • Castillo FI, Penel I, Greppin H. 1984. Peroxidase release induced by ozone in Sedum album leaves. Plant Physiol. 74: 846–851. https://doi.org/10.1104/pp.74.4.846 PMid:16663520 PMCid:PMC1066779

  • Chakraborty K, Singh AL, Kalariya KA, Goswami N, Zala PV. 2015. Physiological responses of peanut (Arachis hypogaea L.) cultivars to water deficit stress: status of oxidative stress and antioxidant enzyme activities. Acta Bot Croat. 74. DOI: https://doi.org/10.1515/botcro-2015-0011.

  • Chen C, Bélanger RR, Benhamou N, Paulitz T. 2000. Defense enzymes induced in cucumber ischolar_mains by treatment with plant growth promoting rhizobacteria (PGPR) and Pythium aphanidermatum. Physiol Mol Plant Pathol. 56: 13–23. https://doi.org/10.1006/pmpp.1999.0243

  • Cronin D, Moönne-loccoz Y, Fenton A, Dunne C, Dowling DN, O’Gara F. 1997. Role of 2,4-diacetylphloroglucinol in the interactions of the biocontrol pseudomonad isolate F113 with the potato cyst nematode Globodera rostochinensis. Appl Environ Microbiol. 63: 1357–1361. PMid:16535571 PMCid:PMC1389549

  • Dey R, Pal KK, Singh SP, Dash P, Parmar V, Chudasma A, Chauhan SM. 2013. Application of DAPG-producing fluorescent pseudomonads for suppressing stem rot pathogen and enhancing yield of groundnut. National Conference of Plant Physiology on “Current Trends in Plant Biology Research”, 13-16 December, Junagadh. pp. 329-330.

  • Gomez KA, Gomez AA. 1984. Statistical Procedure for Agricultural Research. 2nd ed. New York, USA. John Wiley and Sons. 407 pp.

  • Howell CR, Stipanovic RD. 1980. Suppression of Pythium ultimum damping-off of cotton seedlings by Pseudomonas fluorescens and its antibiotic, pyoluteorin. Phytopathology 70: 712–715 https://doi.org/10.1094/ Phyto-70-712

  • Kurabachew H, Wydra K. 2014. Induction of systemic resistance and defense-related enzymes after elicitation of resistance by rhizobacteria and silicon application against Ralstonia solanacearum in tomato (Solanum lycopersicum). Crop Prot. 57: 1–7. https://doi.org/10.1016/j.cropro.2013.10.021

  • Kwak YS, Weller DM. 2013. Take-all of wheat and natural disease suppression: a review. Plant Pathol J. 29: 125–135. https://doi.org/10.5423/PPJ.SI.07.2012.0112 PMid:25288939 PMCid:PMC4174779

  • Liu L, Kloepper JW, Tuzun S. 1995. Induction of systemic resistance in cucumber against Fusarium wilt by plant growth-promoting rhizobacteria. Phytopathology 85: 695–698. https://doi.org/10.1094/Phyto-85-843

  • Malik CP, Singh MB. 1980. Plant Enzymology and Histoenzymology: A text manual. Kalyani Publications, New Delhi. pp. 5. PMid:6784316

  • Okubara PA, Bonsall RF. 2008. Accumulation of Pseudomonas-derived 2,4-diacetylphloroglucinol on wheat seedling ischolar_mains is influenced by host cultivar. Biol Control 46: 322–331. https://doi.org/10.1016/j.biocontrol.2008.03.013

  • Onofri A. 2007. Routine statistical analyses of field experiments by using an Excel extension. Proceedings of 6th National Conference Italian Biometric Society: “La statisticanelle scienze della vita e dell’ambiente”, Pisa. pp. 93-96.

  • Raaijmakers JM, Weller DM, Thomashow LS. 1997. Frequency of antibiotic producing Pseudomonas spp.

  • in natural environments. Appl Environ Microbiol. 63: 881–887. PMid:16535555 PMCid:PMC1389120

  • Raaijmakers JM, Weller DM. 2001. Exploiting genotypic diversity of 2,4-diacetylphloroglucinol-producing Pseudomonas spp.: characterization of superior ischolar_main-colonizing Pseudomonas fluorescens isolate Q8r1-96. Appl Environ Microbiol. 67: 2545–2554. https://doi.org/10.1128/AEM.67.6.2545-2554.2001 PMid:11375162 PMCid: PMC92906

  • Rezzonico F, Moënne-LoccozY, Défago G. 2003. Effect of stress on the ability of a phlA-based quantitative competitive PCR assay to monitor biocontrol strain Pseudomonas fluorescens CHA0. Appl Environ Microbiol. 69: 686–690. https://doi.org/10.1128/AEM.69.1.686690.2003 PMid:12514062 PMCid:PMC152391

  • Sherathia D, Dey R, Thomas M, Dalsania T, Savsani K, Pal KK. 2016. Biochemical and molecular characterization of DAPG-producing Plant Growth-Promoting Rhizobacteria (PGPR) of groundnut (Arachis hypogaea L.). Legume Res. 39: 614–622. https://doi.org/10.18805/lr.v0iOF.9389

  • Siddiqui IA, Shaukat SS. 2002. Rhizobacteria mediated induction of systemic resistance (ISR) in tomato against Meloidogyne javanica. J Phytopathol. 150: 469–473. https://doi.org/10.1046/j.1439-0434.2002.00784.x

  • Siddiqui IA, Shaukat SS. 2003. Suppression of ischolar_mainknot disease by Pseudomonas fluorescens CHAO in tomato: Importance of bacterial secondary metabolic 2,4-diacetylphloroglucinol. Soil Biol Biochem. 35: 1615– 1623. https://doi.org/10.1016/j.soilbio.2003.08.006

  • Timper P, Kone D, Yin J, Pingsheng JI, Brain B. 2009. Evaluation of an antibiotic-producing isolate of Pseudomonas fluorescens for suppression of plantparasitic nematodes. J Nematol. 41: 234–240. PMid: 22736820 PMCid:PMC3380499

  • Wang CX, Ramette A, Punjasamarnwong P, Zala M, Natsch A, Moenne-Loccoz Y, Defago G. 2001. Cosmopolitan distribution of phlD-containing dicotyledonous cropassociated biocontrol pseudomonads of worldwide origin. FEMS Microbiol Ecol. 37: 105–116. https://doi.org/10.1111/j.1574-6941.2001.tb00858.x


Abstract Views: 261

PDF Views: 111




  • Assessment of Nematicidal Properties of Fluorescent Pseudomonads using Peanut Root-Knot Nematode, Meloidogyne arenaria

Abstract Views: 261  |  PDF Views: 111

Authors

Prasanna Holajjer
ICAR-Directorate of Groundnut Research, Junagadh - 362001, Gujarat, India
R. Dey
ICAR-Directorate of Groundnut Research, Junagadh - 362001, Gujarat, India
K. K. Pal
ICAR-Directorate of Groundnut Research, Junagadh - 362001, Gujarat, India
K. Chakraborty
ICAR-Directorate of Groundnut Research, Junagadh - 362001, Gujarat, India
G. Harish
ICAR-Directorate of Groundnut Research, Junagadh - 362001, Gujarat, India
M. V. Nataraja
ICAR-Directorate of Groundnut Research, Junagadh - 362001, Gujarat, India
E. Deepak
ICAR-Directorate of Groundnut Research, Junagadh - 362001, Gujarat, India

Abstract


The primary objective of this study was to identify natural isolates of fluorescent pseudomonads with superior antagonistic activity towards plant parasitic nematodes. Nematicidal potential of eighteen isolates of fluorescent pseudomonads were compared against peanut ischolar_main-knot nematode, Meloidogyne arenaria. Cell-free culture filtrate of DAPG-producing isolates of Pseudomonas putida caused significantly higher mortality of M. arenaria (J2) with highest in isolate DAPG3 (87.36%), followed by DAPG1 (84.16%) compared to other isolates of fluorescent pseudomonads, i.e., P. gessardii BHU1 and P. aeruginosa BM6 after exposure period of 72h at 100% concentration. The selected DAPG-producing isolates of P. putida caused significant inhibition in egg hatching. The lowest cumulative per cent hatch of M. arenaria was observed in the isolate DAPG3 (17.84%) followed by DAPG1 (18.10%). The isolates DAPG1 and DAPG3 also inhibited the nematode invasion in the ischolar_mains of peanut by 41.30% and 36.34%, respectively. Significant reduction in number of galls/plant in peanut ischolar_mains was recorded. The maximum reduction (51.30%) in ischolar_main galling was recorded with combination of seed treatment and soil application of P. putida DAPG1 followed by 41.73% in combined treatment of seed treatment and soil application of P. putida DAPG3. The levels of Peroxidase (POD), Catalase (CAT) and Polyphenol Oxidase (PPO) were non-significant in the leaves of peanut in the treatment that received P. putida DAPG1 and DAPG3, either as seed treatment and/or soil application, compared to inoculated and un-inoculated control. However, significantly enhanced phenol content was recorded in the leaves of peanut in the treatment that received combination of seed treatment and soil application of P. putida DAPG1 and seed treatment alone.

Keywords


Fluorescent Pseudomonads, Meloidogyne arenaria, Nematicidal Activity, Peanut.

References





DOI: https://doi.org/10.18311/jbc%2F2018%2F21359