Journal of Biological Control

Open Access Open Access  Restricted Access Subscription Access

Establishment of Metarhizium anisopliae (Metchnikoff) Sorokin as Endophyte in Maize and Sorghum


Affiliations
1 Division of Insect Ecology, ICAR-National Bureau of Agricultural Insect Resources, H. A. Farm post, Bellary Road, Hebbal, Bengaluru - 560024, Karnataka, India
 

Glasshouse experiments were conducted to establish an indigenous soil-derived strain of Metarhizium anisopliae (ICAR-NBAIR Ma-35) as endophyte in maize and sorghum by artificial inoculation through foliar spray of conidial suspension. Colonization of M. anisopliae were studied at 15, 30, 45, 60, 75 and 90 Days After Treatment (DAT) in stem and leaf tissues of maize and sorghum by plating technique and PCR method. Metarhizium anisopliae showed variation with regard to the extent of colonization in stem and leaf tissues of maize and sorghum. In maize, colonization was observed at 30DAT in stem and 30-45DAT in leaf. In sorghum, colonization of M. anisopliae was observed during 15-60DAT in stem and 15-75DAT in leaf indicating longer persistence compared to maize. Colonization of M. anisopliae was observed in young growing stem tips and leaves (unsprayed) indicating the internal spread of M. anisopliae in maize and sorghum plants. M. anisopliae was not detected in the untreated stem and leaf tissues of maize and sorghum. The positive results of colonization of M. anisopliae in maize and sorghum stem/leaf tissues observed in plating technique were confirmed by the PCR amplification.

Keywords

Endophyte, Entomopathogenic Fungi, Maize, Metarhizium anisopliae, Sorghum.
User
Notifications

  • Akello JT. 2012. Biodiversity of fungal endophytes associated with maize, sorghum and Napier grass and their influence of biopriming on the resistance of leaf mining stem boring and sap sucking insect pests. PhD thesis, University of Bonn, Ecology and Development Series No. 86, ZEF Bonn, pp. 137.

  • Akutse KS, Maniania NK, Fiaboe KKM, Van Den Berg J, Ekesi S. 2013. Endophytic colonization of Vicia faba and Phaseolus vulgaris (Fabaceae) by fungal pathogens and their effects on the life-history parameters of Liriomyza huidobrensis (Diptera: Agromyzidae). Fungal Ecol. 6: 293–301. https://doi.org/10.1016/j.funeco.2013.01.003

  • Anonymous. 2011. Agricultural statistics at a glance. Published by centre for monitoring Indian economy. pp 56-59.

  • Batta YA. 2013. Efficacy of endophytic and applied Metarhizium anisopliae (Metch.) Sorokin (Ascomycota: Hypocreales) against larvae of Plutella xylostella L. (Yponomeutidae: Lepidoptera) infesting Brassica napus plants. Crop Prot. 44: 128–134. https://doi.org/10.1016/j.cropro.2012.11.001

  • Bechara IJ, Destéfano RHR, Bresil C, Messias CL. 2011. Histopathological events and detection of Metarhizium anisopliae using specific primers in infected immature stages of the fruit fly Anastrepha fraterculus (Wiedemann, 1830) (Diptera: Tephritidae). Braz J Biol. 71: 91–98.

  • https://doi.org/10.1590/S1519-69842011000100014 PMid:21437404

  • Bing LA, Lewis LC. 1991. Suppression of Ostrinia nubilalis (Hubner) (Lepidoptera: Pyralidae) by endophytic Beauveria bassiana (Balsamo) Vuillemin. Environ Entomol. 20: 1207–1211. https://doi.org/10.1093/ee/20.4.1207

  • Biswas C, Dey P, Satpathy S, Satya P, Mahapatra BS. 2013. Endophytic colonization of white jute (Corchorus capsularis) plants by different Beauveria bassiana strains for managing stem weevil (Apioncorchori). Phytoparasitica 41: 17–21. https://doi.org/10.1007/ s12600-012-0257-x

  • Brownbridge M, Reay SD, Nelson TL, Glare TR. 2012. Persistence of Beauveria bassiana (Ascomycota: Hypocreales) as an endophyte following inoculation of radiate pine seed and seedlings. Biol Control. 61: 194–200. https://doi.org/10.1016/j.biocontrol.2012.01.002

  • Destéfano RHR, Destéfano SAL, Messias CL. 2004. Detection of Metarhizium anisopliae var. anisopliae within infected sugarcane borer Diatraea saccharalis (Lepidoptera- Pyralidae) using specific primers. Genet Mol Biol. 27: 245–252. https://doi.org/10.1590/S141547572004000200020

  • Dutta P, Kaushik H, Bhowmick P, Puzari KC, Hazarika GN. 2015. Metarhizium anisopliae as endophyte has the ability of plant growth enhancement. Int J Curr Res. 7: 14300–304.

  • Elena GJ, Beatriz PJ, Alejandro P, Roberto L. 2011. Metarhizium anisopliae (Metschnikoff) Sorokin promotes growth and has endophytic activity in tomato plants. Adv Biol Res. 5: 2–27.

  • Gethi M, Mutind AC, Diallo A. 2001. Stem borers in maize: a natural stress and progress towards host plant resistance. Proceedings of the 7th Eastern and Southern Africa Regional Maize Conference; Feb 11–15; Nairobi. Kenya, pp. 45–48.

  • García JE, Posadas JB, Perticari A, Lecuona RE. 2011. Metarhizium anisopliae (Metschnikoff) Sorokin promotes growth and has endophytic activity in tomato plants. Adv Biol Res. 5: 22–27.

  • Greenfield M, Pareja R, Ortiz V, Gómez-Jiménez MI, Vega FE, Parsa S (2015) A novel method to scale up fungal endophyte isolations. Biocontrol Sci Tech. 25: 1208–1212. https://doi.org/10.1080/09583157.2015.1033382

  • Kaushik H, Dutta P. 2016. Establishment of Metarhizium anisopliae, an entomopathogen as endophyte for biological control in tea. Res Crops 17: 375–387. https://doi.org/10.5958/2348-7542.2016.00063.2

  • Kabaluk JT, Ericsson JD. 2007. Metarhizium anisopliae seed treatment increases yield of field corn when applied for wireworm control. Agronomy J. 99: 1377–1381. https://doi.org/10.2134/agronj2007.0017N

  • Khan AL, Hamayun M, Khan SA, Kang S-M, Shinwari ZK, Kamran M, Ur Rehman S, Kim J-G, Lee I-J. 2012. Pure culture of Metarhizium anisopliae LHL07 reprograms soybean to higher growth and mitigates salt stress. World J Microbiol Biotechnol. 28: 1483–1494. https://doi.org/10.1007/s11274-011-0950-9 PMid:22805930

  • Landa BB, López-Díaz C, Jiménez-Fernández D, MontesBorrego M, Mu-oz-Ledesma FJ, Ortiz-Urquiza A,Quesada Moraga E. 2013. In-planta detection and monitorization of endophytic colonization by a Beauveria bassiana strain using a new-developed nested and quantitative PCRbased assay and confocal laser scanning microscopy. J Invertebr Pathol. 114: 128–138. https://doi.org/10.1016/j.jip.2013.06.007 PMid:23851123

  • Ling J, Ling KLE, Chan KW, French GL. 1987. Computer programs for accurate determination of size of DNA fragments in agarose gels. J Clin Pathol. 40: 692–695. https://doi.org/10.1136/jcp.40.6.692 PMid:3611397 PMCid:PMC1141066

  • Mantzoukas S, Chondrogiannis C, Grammatikopoulos G. 2015. Effects of three endophytic entomopathogens on sweet sorghum and on the larvae of the stalk borer Sesamia nonagrioides. Entomol Exp Appl. 154: 78–87. https://doi.org/10.1111/eea.12262

  • Mutune B, Ekesi S, Niassy S, Matiru V, Bii C, Maniania NK. 2016. Fungal endophytes as promising tools for the management of bean stem maggot Ophiomyia phaseoli on beans Phaseolus vulgaris. J Pest Sci. 89: 993–1001. https://doi.org/10.1007/s10340-015-0725-4

  • Parsa S, García-Lemos AM, Castillo K, Ortiz V, Becerra López- Lavalle LA, Braun J, Vega FE. 2016. Fungal endophytes in germinated seeds of the common bean, Phaseolus vulgaris. Fungal Biol. 120: 783–790. https://doi.org/10.1016/j.funbio.2016.01.017 PMid:27109374 PMCid:PMC4857701

  • Parsa S, Ortiz V, Gómez-JiménezMI, KramerM, Vega FE. 2018. Root environment is a key determinant of fungal entomopathogen endophytism following seed treatment in the common bean, Phaseolus vulgaris.

  • Biol Control. 116: 74–81. https://doi.org/10.1016/j.biocontrol.2016.09.001

  • Ramanujam B, Poornesha B, Yathish KR, Renuka S. 2015. Evaluation of pathogenicity of different isolates of Metarhizium anisopliae (Metchnikoff) Sorokin on maize stem borer Chilo partellus (Swinhoe) using laboratory bioassay method. Biopestic Int. 11: 89–95.

  • Razinger J, Lutz M, Schroers H-J, Palmisano M, Wohler C, Urek G, Grunder J. 2014. Direct plantlet inoculation with soil or insect-associated fungi may control cabbage ischolar_main fly maggots. J Invertebr Pathol. 120: 58–66. https://doi.org/10.1016/j.jip.2014.05.006 PMid:24907449

  • Sarup P, Siddiqui KH, Marwaha KK. 1987. Trends in maize pest management research in India together with bibliography. J Entomol Res. 11: 19–68.

  • Sekhar JC, Rakshit S, Kumar P, Anuradha MM, Dass S. 2008. Differential reaction of CIMMYT Maize lines and their hybrid combinations to pink borer, Sesamia inferens Walker. Ann Plant Prot Sci. 16: 404–406.

  • Tefera T, Vidal S. 2009. Effect of inoculation method and plant growth medium on endophytic colonization of sorghum by the entomopathogenic fungus Beauveria bassiana. BioControl 54: 663–669. https://doi.org/10.1007/s10526-009-9216-y


Abstract Views: 279

PDF Views: 141




  • Establishment of Metarhizium anisopliae (Metchnikoff) Sorokin as Endophyte in Maize and Sorghum

Abstract Views: 279  |  PDF Views: 141

Authors

B. Ramanujam
Division of Insect Ecology, ICAR-National Bureau of Agricultural Insect Resources, H. A. Farm post, Bellary Road, Hebbal, Bengaluru - 560024, Karnataka, India
B. Poornesha
Division of Insect Ecology, ICAR-National Bureau of Agricultural Insect Resources, H. A. Farm post, Bellary Road, Hebbal, Bengaluru - 560024, Karnataka, India

Abstract


Glasshouse experiments were conducted to establish an indigenous soil-derived strain of Metarhizium anisopliae (ICAR-NBAIR Ma-35) as endophyte in maize and sorghum by artificial inoculation through foliar spray of conidial suspension. Colonization of M. anisopliae were studied at 15, 30, 45, 60, 75 and 90 Days After Treatment (DAT) in stem and leaf tissues of maize and sorghum by plating technique and PCR method. Metarhizium anisopliae showed variation with regard to the extent of colonization in stem and leaf tissues of maize and sorghum. In maize, colonization was observed at 30DAT in stem and 30-45DAT in leaf. In sorghum, colonization of M. anisopliae was observed during 15-60DAT in stem and 15-75DAT in leaf indicating longer persistence compared to maize. Colonization of M. anisopliae was observed in young growing stem tips and leaves (unsprayed) indicating the internal spread of M. anisopliae in maize and sorghum plants. M. anisopliae was not detected in the untreated stem and leaf tissues of maize and sorghum. The positive results of colonization of M. anisopliae in maize and sorghum stem/leaf tissues observed in plating technique were confirmed by the PCR amplification.

Keywords


Endophyte, Entomopathogenic Fungi, Maize, Metarhizium anisopliae, Sorghum.

References





DOI: https://doi.org/10.18311/jbc%2F2018%2F21580